Anti sorcs2

Manufactured by R&D Systems

Sourced in United States

Anti-SorCS2 is a polyclonal antibody that recognizes the SorCS2 protein. SorCS2 is a member of the Vps10p-domain receptor family and functions as a sorting receptor for neurotrophin receptors.

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Lab products found in correlation

Anti synaptophysin, by Synaptic Systems (1 mentions) Anti psd95, by Cell Signaling Technology (1 mentions) Anti actin, by Abcam (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti rab11, by Cell Signaling Technology (1 mentions) Anti ho 1, by Cell Signaling Technology (1 mentions) Anti eaat3, by Cell Signaling Technology (1 mentions) Mab2263, by Merck Group (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti gfp, by MBL Life Science (1 mentions) Anti map2, by Abcam (1 mentions) Alexa fluor 546 phalloidin, by Thermo Fisher Scientific (1 mentions) Anti psd95, by Merck Group (1 mentions) Latrunculin a, by Cayman Chemical (1 mentions) Anti bassoon, by Enzo Life Sciences (1 mentions) Alexa fluor antibodies, by Thermo Fisher Scientific (1 mentions) Anti fascin, by Abcam (1 mentions)

2 protocols using anti sorcs2

Comprehensive Western Blot Analysis

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Tissue and cell lysates were analyzed by western blot using the following antibodies: anti-SorCS2 (R&D Systems, AF4237, 1:1000), anti-EAAT3 (Cell Signaling, #14501, 1:1000), anti-p-ERK (Cell Signaling, #4370, 1:2000), anti-tubulin (EMD Millipore, CP06, 1:5000), anti-mGlur2/3 (Novus Biologicals, NB300-124, 1:1000), anti-actin (Abcam, ab8227, 1:2000), anti-JWA (Trans Genic, KR057, 1:250), anti-N-cadherin (Cell Signaling, #14215, 1:1000), anti-GFP (MBL International, MBL598, 1:2000), anti-Rab11 (Cell Signaling, #5589, 1:1000), anti-synaptophysin (Synaptic Systems, 101011, 1:5000), anti-GluA1 (EMD Millipore, MAB2263, 1:1000), anti-GluA2 (EMD Millipore, MABN71, 1:1000), anti-PSD95 (Cell Signaling, #3409, 1:1000), anti-HO-1 (Cell Signaling, #70081, 1:1000). After incubation with secondary antibodies coupled to HRP, chemiluminescent signal was registered with use of digital LI-COR imaging system.

Malik A.R., Szydlowska K., Nizinska K., Asaro A., van Vliet E.A., Popp O., Dittmar G., Fritsche-Guenther R., Kirwan J.A., Nykjaer A., Lukasiuk K., Aronica E, & Willnow T.E. (2019). SorCS2 Controls Functional Expression of Amino Acid Transporter EAAT3 and Protects Neurons from Oxidative Stress and Epilepsy-Induced Pathology. Cell Reports, 26(10), 2792-2804.e6.

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Antibody Characterization of Neuronal Proteins

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The following primary antibodies were used: anti-SorCS2 (R&D systems, Minneapolis, MN, USA), anti-p75 9992 (a gift from Dr. Moses Chao; Huber and Chao, 1995), anti-Trio (Santa Cruz Biotechnology, Inc., Dallas, TX), anti-fascin (Abcam, Cambridge, MA USA), anti-phospho S39 fascin (Abcam), anti-MAP2 (Abcam), anti-PSD-95 (Sigma, St. Louis, MO, USA), and anti-Bassoon (Enzo Life Sciences, Farmingdale, NY, USA). Secondary antibodies were Alexa Fluor antibodies (Life Technologies, Norwalk, CT, USA), except for the 405nm fluorescent DyLight (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). For visualization of actin cytoskeleton, Alexa Fluor 546 phalloidin (Life Technologies, Thermo Fisher Scientific) was used. Latrunculin A was used for actin depolymerization experiments (Cayman Chemicals, Ann Arbor, MI).

Giza J.I., Kim J., Meyer H.C., Anastasia A., Dincheva I., Zheng C.I., Lopez K., Bains H., Yang J., Bracken C., Liston C., Jing D., Hempstead B.L, & Lee F.S. (2018). The BDNF Val66Met prodomain disassembles dendritic spines altering fear extinction circuitry and behavior. Neuron, 99(1), 163-178.e6.

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