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Alanine transaminase activity assay kit

Manufactured by Merck Group
Sourced in United States

The Alanine Transaminase Activity Assay Kit is a laboratory tool designed to quantify the activity of the enzyme alanine transaminase (ALT) in biological samples. ALT is an important marker for liver function, and this kit provides a standardized method to measure its activity.

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2 protocols using alanine transaminase activity assay kit

1

Curcumin-based Enzyme Activity Assays

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DOX (Sigma-Aldrich in St. Louis, MO (USA).
Powdered Curcuma longa (turmeric) was used to make CMX (CAS Number: 458-37-7; Sigma). Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134).
Aspartate Aminotransferase Activity Assay Kit (ab105135).
MDA (Assay Kit) (Sigma-Aldrich in St. Louis, MO (USA)).
Abcam provided the CAT (ab83464 Assay Kit) and SOD (ab65354 Assay Kit). TNF-α: derived from Santa Cruz Biotechnology Inc., Dallas, Texas (sc-28318).
Serum CK-MB (Catalogue No.: MBS2515061) and troponin (Catalogue No.: MBS038739) were determined using specific ELISA kits supplied by MyBioSource (San Diego, USA).
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2

Passion Fruit Peel Bioactive Extraction

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The materials used in this research were purple passion fruit peel, red passion fruit peel, yellow passion fruit peel, ethanol (E-Merck), ethylene diamine tetraacetic acid (E-Merck), alanine transaminase activity assay kit (Sigma Aldrich), aspartate transaminase activity assay kit (Sigma Aldrich), urea assay kit (Sigma Aldrich), creatinine assay kit (Sigma Aldrich), sodium carboxymethyl cellulose (Tokyo Chemical Industry), and demineralized water (Brataco).
The tools used in this research were cutter (Kenko), drying cabinet (Alumex), blender (Miyako), balance (Mettler Toledo), analytical balance (Mettler Toledo), filter paper (Whatman), dropper (Iwaki), evaporating dish (Iwaki), maceration vessel (Iwaki), beaker glass (Iwaki), measuring glass (Iwaki), volumetric flask (Iwaki), test tube (Iwaki), hot plate stirrer (Thermo), water bath (Memmert), incubator (Memmert), rotary evaporator (Buchi), thermometer (Lutron), test sieve 10 (Retsch), stopwatch (Casio), spectrophotometer (Agilent).
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