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Glc 1 p

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Glc-1-P is a chemical compound that serves as a precursor for the synthesis of various biomolecules. It is a glucose-1-phosphate molecule, which is a key intermediate in carbohydrate metabolism. Glc-1-P plays a crucial role in the conversion of glucose to glycogen and other polysaccharides.

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Lab products found in correlation

Adenosine triphosphate (atp), by Merck Group (3 mentions) Gal 1p, by Merck Group (2 mentions) Glc 6 p, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions) Sodium methoxide, by Tokyo Chemical Industry (1 mentions) Kh2po4, by Fujifilm (1 mentions) Fru 1 6 bisp, by Merck Group (1 mentions) Gdp glc, by Merck Group (1 mentions) Inorganic pyrophosphate ppi, by Merck Group (1 mentions) Udp glc, by Merck Group (1 mentions) Fru6p, by Merck Group (1 mentions) Glc 6 p dehydrogenase, by Merck Group (1 mentions) Oligonucleotide, by Merck Group (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (1 mentions) Glcn6p, by Merck Group (1 mentions)

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Glucose-1-phosphate (Glc-1-P) (2 citations)

7 protocols using glc 1 p

1

Enzymatic Amylose Synthesis with Thermostable Phosphorylase

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(Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) in a solution of sodium methoxide with methanol. Thermostable α-glucan phosphorylase from Aquifex aeolicus VF5 was supplied from Ezaki Glico Co., Ltd. (Osaka, Japan) [20, (link)34, (link)35 (link)]. An amylose sample was prepared by the thermostable α-glucan phosphorylase-catalyzed enzymatic polymerization of Glc-1-P. All other commercial available reagents and solvents-i.e., Glc-1-P (disodium salt, Sigma-Aldrich, St. Louis, MO, USA), Glc 3 (Hayashibara CO., LTD., Okayama, Japan), Tris-acetate buffer (Nacalai Tesque, Inc., Kyoto, Japan), KH 2 PO 4 (FUJIFILM Wako Pure Chemical Co., Osaka, Japan), and dimethyl sulfoxide (DMSO, FUJIFILM Wako Pure Chemical Co., Osaka, Japan)-were used as received.
Kadokawa J., Nakamura S., & Yamamoto K. (2020). Thermostable α-Glucan Phosphorylase-Catalyzed Enzymatic Copolymerization to Produce Partially 2-Deoxygenated Amyloses. Processes, 8(9), 1070-1070.
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2

Enzymatic Assays for Carbohydrate Metabolism

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Glc-1P, Gal-1P, GlcN-1P, ATP, ITP, UTP, CTP, GTP, ADP-Glc, UDP-Glc, GDP-Glc, inorganic pyrophosphate (PPi) and Fru-1,6-bisP were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of the highest quality available.
Ebrecht A.C., Solamen L., Hill B.L., Iglesias A.A., Olsen K.W, & Ballicora M.A. (2017). Allosteric Control of Substrate Specificity of the Escherichia coli ADP-Glucose Pyrophosphorylase. Frontiers in Chemistry, 5, 41.
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3

Sourcing and Characterization of Sugar-1-Phosphates

Cited 1 time
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All sugar-1-phosphates, except D-Glc-1-P, fructose-phosphates and α-D-glucosamine-1-P [GlcN-1-P], were kindly provided by Dr. Motomitsu Kitaoka, from Laboratory of Enzyme Research, NARO, Ibaraki, Japan. Separate batches of Gal-1-P were also purchased from Sigma–Aldrich (Stockholm, Sweden) and from Carbosynth (Compton, United Kingdom). Glc-1-P, glucosamine-1-P (GlcN-1-P) as well as β-D-fructofuranosyl-1-P (β-D-Fruf-1-P), β-D-fructofuranosyl-2-P (β-D-Fruf-2-P) and other fructose-phosphates were from Sigma–Aldrich. Compound #41 (ZINC720558), an inhibitor of Trypanosoma brucei UAGPase2 (Urbaniak et al., 2013 (link)), was purchased from MolPort (Riga, Latvia), whereas cmp #6D (CID 6526371) was from (ChemBridge Inc., San Diego, CA, United States).
Decker D, & Kleczkowski L.A. (2017). Substrate Specificity and Inhibitor Sensitivity of Plant UDP-Sugar Producing Pyrophosphorylases. Frontiers in Plant Science, 8, 1610.
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4

Biochemical Reagents for Research

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ATP, Fru6P, 3PGA, and Glc1P were from Sigma Aldrich (St. Louis, MO, USA). All other reagents were of the highest quality available.
Ferrero D.M., Piattoni C.V., Asencion Diez M.D., Rojas B.E., Hartman M.D., Ballicora M.A, & Iglesias A.A. (2020). Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development. Frontiers in Plant Science, 11, 1058.
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5

Fungus Cultivation and Spore Quantification

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Glc-1P, Glc-6P dehydrogenase, and Leuconostoc mesenteroides (LmG6PDH) were obtained from Sigma-Aldrich. Isothiazolone derivatives (ISFP1, ISFP2, ISFP3, ISFP4) were purchased from Maybridge and ISFP7 from Sigma-Aldrich. The parental strain of A. fumigatus KU80 ΔpyrG- was cultivated at 37 °C on solid CM supplemented with 5 mM uridine and 5 mM uracil. Spore concentration was calculated using a hemocytometer.
Yan K., Stanley M., Kowalski B., Raimi O.G., Ferenbach A.T., Wei P., Fang W, & van Aalten D.M. (2022). Genetic validation of Aspergillus fumigatus phosphoglucomutase as a viable therapeutic target in invasive aspergillosis. The Journal of Biological Chemistry, 298(6), 102003.
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6

Preparation of Biochemical Reagents

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Protein standards, antibiotics, IPTG, Glc-1P, Glc-6P, glucosamine-1P (GlcN-1P), galactose-1P (Gal-1P), N-acetylglucosamine-1P (GlcNAc-1P), 6-phosphogluconate (6-PGlcA), ATP, UTP, GTP, CTP, fructose-6P (Fru-6P), ribose-5P (Rib-5P), phosphoenolpyruvate (PEP), pyruvate (Pyr), NADPH, and oligonucleotides were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of the highest quality available.
Cereijo A.E., Asencion Diez M.D., Dávila Costa J.S., Alvarez H.M, & Iglesias A.A. (2016). On the Kinetic and Allosteric Regulatory Properties of the ADP-Glucose Pyrophosphorylase from Rhodococcus jostii: An Approach to Evaluate Glycogen Metabolism in Oleaginous Bacteria. Frontiers in Microbiology, 7, 830.
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7

Enzymatic Phosphate Compound Acquisition

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Glc-1-P, Glc-6-P, glucose-1,6-bisphosphate (Glc-1,6-2P) and GlcN-6-P were purchased from Sigma-Aldrich. GlcNAc-6-P, GlcNAc-1-P and GlcN-1-P were purchased from Santa Cruz Biotechnology. Double distilled water was used for HPLC, and all other solvents and chemicals were HPLC grade.
Jia X., Kang J, & Yin H. (2016). A simple and rapid method for measuring α-D-phosphohexomutases activity by using anion-exchange chromatography coupled with an electrochemical detector. PeerJ, 4, e1517.
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