Human hepatoma cell line, HepG2, was freshly purchased from ATCC (cat HB-8065) and the cell line authentication test conducted by ATCC confirmed a match percentage exceeding the 80% threshold. Cells were cultured in RPMI media (Gibco, cat 11875-093) with 10% FBS. To test the effect of MCT1 depletion, cells were plated in 6 well plates (300k cells/well) or 12-well plates (150k cells/well). The next day, cells were transfected with either NTC-siRNA or MCT1-siRNA using Lipofectamine RNAi Max (Thermo Fisher, cat 13778075) for 6 hr in less serum optiMEM media (Thermo Fisher, cat 31985062). After 48 hr, HepG2 cells were harvested. The media were saved to further test for secreted factors that may affect hepatic stellate cell activation. LX2 cells were incubated with the conditioned media (40% conditioned media+60% fresh media), and cells were harvested after 48 hr.
Less serum optimem media
Manufactured by Thermo Fisher Scientific
Less serum optiMEM media is a cell culture medium designed to provide an optimal environment for the growth and maintenance of various cell types while reducing the amount of serum required. It is formulated to support cell proliferation and viability in a wide range of applications.
Automatically generated - may contain errors
2 protocols using less serum optimem media
1
Evaluating MCT1 Depletion Effects on HepG2 Cells
2
Investigating MCT1 Depletion Effects on Hepatocytes
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Human hepatoma cell line, HepG2, was purchased from ATCC, cat HB-8065. Cells were cultured in RPMI media (Gibco, cat 11875-093) with 10% FBS. To test the effect of MCT1 depletion, cells were plated in 6 well plates (300k cells/well) or 12 well plates (150k cells/well). The next day, cells were transfected with either NTC-siRNA or MCT1-siRNA using lipofectamine RNAi Max (Thermofisher, cat 13778075) for 6 hours in less serum optiMEM media (Thermofisher, cat31985062). After 48 hours, HepG2 cells were harvested. The media were saved to further test for secreted factors that may affect hepatic stellate cell activation. LX2 cells were incubated with the conditioned media (40% conditioned media + 60% fresh media), and cells were harvested after 48 hours.
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