We used a Zeiss 700 Axio Imager 2 laser scanning confocal for all images. DNA-FISH images were taken at a 0.4 µm step size, with pinholes that were adjusted to have equal optical section thickness in all channels.
700 axio imager 2
Manufactured by Zeiss
The Zeiss 700 Axio Imager 2 is a microscope system designed for advanced research and imaging applications. It features a high-performance optical system, advanced illumination, and a modular design to accommodate various imaging techniques and accessories.
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3 protocols using 700 axio imager 2
1
Zeiss 700 Axio Imager 2 Confocal Imaging
2
Confocal Imaging of DNA-FISH and ParB-ParS
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3D imaging was done on a Zeiss 700 Axio Imager 2 laser scanning confocal. DNA-FISH and ParB-ParS images were taken at a 0.4μm step size, and pinholes were adjusted to have equal optical section thickness for all channels.
3
Confocal Imaging of DNA-FISH and ParB-ParS
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