Grace s insect cell medium

Manufactured by Thermo Fisher Scientific

Sourced in United States

Grace's insect cell medium is a cell culture medium specifically designed for the growth and maintenance of insect cells. It provides the necessary nutrients and growth factors to support the cultivation of various insect cell lines.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Grace’s insect cell culture medium Grace’s insect medium Grace insect medium Grace’s medium Grace medium

2 protocols using grace s insect cell medium

Culturing Spodoptera frugiperda Sf9 Cells

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Spodoptera frugiperda (Sf9) was cells purchased from National Cell bank of Iran (Pasteur Institute of Iran) and used for virus transfection.
Sf9 cells adapted to grow in Grace’s insect cell medium (Invitrogen, USA) containing 10% fetal bovine serum (FBS) (Gibco, USA) and
1% penicillin-streptomycin (Gibco, USA). Cells were maintained at 27 °C in a non-humidified incubator. Sf9 cells cultured in T-25 flask and
after 3-4 days reached 90-100% confluency. Cells were washed with PBS and detached with a scraper before transfection.

Shokrollahi N., Habibi-Anbouhi M., Jahanian-Najafabadi A., Alirahimi E, & Behdani M. (2021). Expressing of Recombinant VEGFR2-specific Nanobody in Baculovirus Expression System. Iranian Journal of Biotechnology, 19(1), e2783.

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Culturing Sf9 Cells with AuNPs

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S. frugiperda Sf9 (ATCC CRL-1711) insect cells were cultured in Grace's insect cell medium (Invitrogen, Carlsbad, CA) and supplemented with 10% FBS (Thermo Fisher, Rockford, IL) and 1% P/S/A antibiotics (Biological Industries, Beit-Haemek, Israel) at 27 °C. 100 nm gold nanoparticle (AuNP) solution was purchased from Sigma-Aldrich (St. Louis, MO).

Chen H.W., Huang C.Y., Lin S.Y., Fang Z.S., Hsu C.H., Lin J.C., Chen Y.I., Yao B.Y, & Hu C.M. (2016). Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection. Biomaterials, 106, 111-118.

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