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Dichloromethane dcm n n dimethylformamide dmf

Manufactured by Avantor
Sourced in United States

Dichloromethane (DCM) is a colorless, volatile liquid commonly used as a solvent. N,N-dimethylformamide (DMF) is a clear, colorless liquid that serves as a versatile polar aprotic solvent. Both products are widely used in various industrial and laboratory applications.

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2 protocols using dichloromethane dcm n n dimethylformamide dmf

1

Synthesis and Characterization of CSPIO

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mPEG (Mn 2000) and l-alanine were purchased from Sigma-Aldrich (St Louis, MO, USA). Trifluoroacetic acid-d (TFA-d) and trifluoroacetic acid (TFA) were purchased from Alfa Aesar (Wall Hill, MA, USA). Ammonium hydroxide solution (28–30%), diethyl ether, toluene, hexane, and ethanol (95%) were purchased from Echo Chemicals (Toufen, Miaoli, Taiwan). Dimethyl sulfoxide (DMSO) was obtained from J.T. Baker (Center Valley, PA, USA). Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), and antimycotic antibiotics were purchased from Gibco (Grand Island, NY, USA). Tetrahydrofuran (THF) was purchased from TEDIA (Fairfield, OH, USA); dichloromethane (DCM), N,N-dimethylformamide (DMF), and chloroform were obtained from AVANTOR (Center Valley, PA, USA) and dried over CaH2 before being used. CSPIO was prepared via a chemical co-precipitation method [30 (link)].
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2

Synthesis and Characterization of Chitosan-Coated Superparamagnetic Iron Oxide Nanoparticles

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ʟ-alanine and mPEG (Mn 2000) were purchased from Sigma-Aldrich (St Louis, MO, USA). Trifluoroacetic acid (TFA) and trifluoroacetic acid-d (TFA-d) were purchased from Alfa Aesar (Wall Hill, MA, USA). Ammonium hydroxide solution (28–30%), toluene, hexane, diethyl ether, and 95% ethanol were purchased from Echo Chemicals (Toufen, Miaoli, Taiwan). Dimethyl sulfoxide (DMSO) was purchased from J.T. Baker (Center Valley, PA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antimycotic antibiotics were purchased from Gibco (Grand Island, NY, USA). Tetrahydrofuran (THF) was purchased from TEDIA (Fairfield, OH, USA); dichloromethane (DCM), N,N-dimethylformamide (DMF), and chloroform were obtained from AVANTOR (Center Valley, PA, USA) and dried over CaH2 before being used. Chitosan-coated superparamagnetic iron oxide (CSPIO) was prepared with a chemical co-precipitation method [29 (link)]. Prussian blue and anti-CD31 antibody were purchased from Thermo Fisher (Cheshire, UK). Anti-Ki67 antibody and anti-alpha smooth muscle actin (SMA) antibody were purchased from Abcam (Cambridge, UK).
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