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Ap conjugated anti mouse igm and igg3 antibodies

Manufactured by Southern Biotech
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AP-conjugated anti-mouse IgM and IgG3-antibodies are laboratory reagents used for the detection and analysis of mouse immunoglobulin M (IgM) and immunoglobulin G3 (IgG3) in various immunoassays and research applications. The antibodies are conjugated with alkaline phosphatase (AP), which serves as a reporter enzyme for colorimetric or fluorometric detection.

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Lab products found in correlation

96 well immune plate, by Thermo Fisher Scientific (2 mentions) Np25 bsa, by Biosearch Technologies (2 mentions) P nitrophenylphosphate, by Southern Biotech (2 mentions) Mouse igm and igg3, by Southern Biotech (2 mentions)

Spelling variants of this product

Anti-IgG3 (8 citations) Anti-mouse IgG3 (7 citations) Anti-IgM-AP (3 citations) IgM-AP (3 citations) Mouse IgM and IgG3 (2 citations) Anti-Mouse IgM-AP (2 citations) IgG3-AP (2 citations) IgG3 antibodies (2 citations)

2 protocols using ap conjugated anti mouse igm and igg3 antibodies

1

NP-BSA Immunization Antibody Assay

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For NP-LPS and NP-Ficoll immunization experiments, 96 well immune-plates (Thermo Fisher Scientific) were coated with NP25-BSA (Biosearch Technologies). Mouse serum samples were incubated for 2h at RT. Standard curves were generated using mouse IgM and IgG3 (Southern Biotech). Bound antibodies were detected by AP-conjugated anti-mouse IgM and IgG3-antibodies (Southern Biotech). Plates were developed with p-nitrophenylphosphate (Southern Biotech) dissolved in substrate buffer.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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2

NP-BSA Immunization Antibody Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For NP-LPS and NP-Ficoll immunization experiments, 96 well immune-plates (Thermo Fisher Scientific) were coated with NP25-BSA (Biosearch Technologies). Mouse serum samples were incubated for 2h at RT. Standard curves were generated using mouse IgM and IgG3 (Southern Biotech). Bound antibodies were detected by AP-conjugated anti-mouse IgM and IgG3-antibodies (Southern Biotech). Plates were developed with p-nitrophenylphosphate (Southern Biotech) dissolved in substrate buffer.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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