Particle isolation from the lubricant used in the POF wear test was performed by digesting the lubricant with 10.5 M HCl for 90 min at 50 °C, followed by subsequent dilution with methanol (99.9% minimum, Optima™, Thermo Fisher Scientific, Waltham, MA, USA) and filtration through a 0.1 μm pore polycarbonate membrane (Nucleopore®-Track Etched, Whatman, Florham Park, NJ, USA). Photoshop CS5 (Softonic International S.A., Barcelona, Spain) and ImageJ (U.S. National Institutes of Health, Bethesda, MD, USA) were used to analyze the SEM micrographs for particle area and perimeter, from which the equivalent circle diameter (ECD) and shape ratio of the particles were calculated using the formulas in Sprecher et al. [13 ]. Particles were defined as rounded, elongated, or fibrillar based on their length-to-width ratio: <2, 2 to 5, or greater than 5, respectively.
Nucleopore track etched
Manufactured by Cytiva
Sourced in United States
The Nucleopore®-Track Etched is a laboratory equipment product manufactured by Cytiva. It is designed for membrane filtration applications. The product utilizes a track-etched polymer membrane technology to create uniform and precise pore sizes for various filtration needs. The core function of the Nucleopore®-Track Etched is to provide a reliable and consistent filtration solution for scientific and research applications.
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2 protocols using nucleopore track etched
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Particle Isolation and Characterization from Lubricant
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Particle Isolation and Characterization from Lubricant
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Particle isolation from the lubricant used in the POF wear test was performed by digesting the lubricant with 10.5 M HCl for 90 min at 50 °C, followed by subsequent dilution with methanol (99.9% minimum, Optima™, Thermo Fisher Scientific, Waltham, MA, USA) and filtration through a 0.1 μm pore polycarbonate membrane (Nucleopore®-Track Etched, Whatman, Florham Park, NJ, USA). Photoshop CS5 (Softonic International S.A., Barcelona, Spain) and ImageJ (U.S. National Institutes of Health, Bethesda, MD, USA) were used to analyze the SEM micrographs for particle area and perimeter, from which the equivalent circle diameter (ECD) and shape ratio of the particles were calculated using the formulas in Sprecher et al. [13 ]. Particles were defined as rounded, elongated, or fibrillar based on their length-to-width ratio: <2, 2 to 5, or greater than 5, respectively.
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