A polyethylene nonwoven fabric (Tyvek® 1073B, DuPont, Wilmington, DE, USA), with a thickness of 180 µm, was used as a bFGF adsorbent. This fabric comprised of randomly oriented microfibers with a diameter of 0.5–10 µm (4 µm on average) and has a microporous structure with a Gurley-Hill porosity of 22 s/100 cc (range: 8–36 s/100 cc) according to the manufacturer’s product information sheet. As a bFGF source, 1 mg/mL human recombinant bFGF (bFGF AF, Katayama Chemical Industries Co., Ltd., Osaka, Japan) was used. For diluting the bFGF source and rinsing the bFGF-adsorbed membranes, phosphate-buffered saline (PBS) (D-PBS(–), FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was used.
Tyvek 1073b
Manufactured by DuPont
Sourced in United States
Tyvek® 1073B is a high-performance spunbonded olefin material manufactured by DuPont. It is designed to provide a durable and protective barrier for various applications. The core function of Tyvek® 1073B is to serve as a reliable and versatile material for a wide range of laboratory and industrial uses.
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Lab products found in correlation
2 protocols using tyvek 1073b
1
Polyethylene Nonwoven Fabric for bFGF Adsorption
2
Preparation of PE Nonwoven Fabric Sheets
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We used a 180 μm-thick water-floatable PE nonwoven fabric sheet (Tyvek® 1073B; DuPont, USA) generously supplied by DuPont-Asahi Flash Spun Products Co., Ltd., Japan. The sheet comprises 100% high-density PE microfibers with a diameter of 0.5–10 μm (average diameter of 4 μm), randomly oriented and bound to one another by thermal pressing. Additionally, the sheet is air- and moisture-permeable and has a basis weight of 74.6 g m−2 (range: 71.2–78.0 g m−2) and a Gurley–Hill porosity of 22 s/100 cm3 (range: 8–36 s/100 cm3) according to the manufacturer's product information sheet.
The as-received PE nonwoven fabric sheet was cut into 10 mm × 10 mm using a lever-controlled sample cutter (SDL-200; DUMBBELL Co., Ltd., Japan), ultrasonically washed with ultrapure water and ethanol, dried in air, and stored in a desiccator before further use. The thus-prepared sheet pieces are hereafter referred to as P00 sheets.
The as-received PE nonwoven fabric sheet was cut into 10 mm × 10 mm using a lever-controlled sample cutter (SDL-200; DUMBBELL Co., Ltd., Japan), ultrasonically washed with ultrapure water and ethanol, dried in air, and stored in a desiccator before further use. The thus-prepared sheet pieces are hereafter referred to as P00 sheets.
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