Lumat3 lb9508 luminometer

Manufactured by Berthold Technologies

Sourced in Germany

The Lumat3 LB9508 is a luminometer manufactured by Berthold Technologies. It is a compact, high-performance instrument designed for the measurement of luminescence in various applications. The core function of the Lumat3 LB9508 is to quantify light emission from luminescent samples, providing precise and reliable data.

Automatically generated - may contain errors

Lab products found in correlation

Mrx revelation, by Dynex (1 mentions) Pcbfre luc, by Addgene (1 mentions) Psv40 rluc, by Promega (1 mentions) Pei transfection reagent, by Polyplus Transfection (1 mentions) Mirna mimics, by Promega (1 mentions) K2 transfection reagent, by Biontex (1 mentions) Renilla luciferase assay system, by Promega (1 mentions)

5 protocols using lumat3 lb9508 luminometer

Measurement of Superoxide Radical

Check if the same lab product or an alternative is used in the 5 most similar protocols

Measurement of O₂⁻ generation was carried out by Lumat³ LB9508 luminometer (Berthold Technologies, Bad Wildbad, Germany) using Diogenes-luminol chemiluminescence probes as described previously [[16] , [17] (link), [18] ].

Kikuchi H., Harata K., Madhyastha H, & Kuribayashi F. (2021). Ellagic acid and its fermentative derivative urolithin A show reverse effects on the gp91-phox gene expression, resulting in opposite alterations in all-trans retinoic acid-induced superoxide generating activity of U937 cells. Biochemistry and Biophysics Reports, 25, 100891.

+ Open protocol

+ Expand

Luciferase Assay for CSL-Driven Transcription

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luciferase reporter vector with a promoter containing four CSL-binding elements was purchased from Addgene (Cambridge, MA, USA) (pCBFRE-luc, plasmid#26897). The luciferase assay was conducted 36 h posttransient transfection. Lysis buffer was purchased from Promega (Madison, WI, USA) (E3971), and 20 of 150 μL of the lysis supernatant were used with a Lumat3 LB9508 luminometer (Berthold, Oak Ridge, TN, USA) for the measurement of luciferase activity. Each sample was measured three times. All results were normalized to β-galactosidase activity (MRX Revelation; DYNEX Technologies, CHANTILLY, VA, USA).

Liu B., Lin X., Yang X., Dong H., Yue X., Andrade K.C., Guo Z., Yang J., Wu L., Zhu X., Zhang S., Tian D., Wang J., Cai Q., Chen Q., Mao S., Chen Q, & Chang J. (2015). Downregulation of RND3/RhoE in glioblastoma patients promotes tumorigenesis through augmentation of notch transcriptional complex activity. Cancer Medicine, 4(9), 1404-1416.

+ Open protocol

+ Expand

Myogenin Promoter and 3' UTR Luciferase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the myogenin promoter reporter assay, C2C12 cells were transfected with myogenin promoter luciferase reporter plasmids (100 ng), miRNA mimics (100 µM), and in-house modified SV40 promoter-driven Renilla luciferase vector (pSV40-R.Luc; Promega, Madison, WI, USA) as an internal control. For the 3' UTR reporter assay, C2C12 cells were transfected with 3' UTR reporter plasmids (100 ng) and miRNA mimics (100 µM). The miRNA mimics (RiboBio, Guangzhou, Guangdong, China) were transfected into C2C12 cells using K2 transfection reagent (Biontex Laboratories GmbH, München, Germany) following the manufacturer's instructions. PEI transfection reagent (Polyplus, Illkirch, France) was used for the transfection of plasmids. The luciferase activity was measured using a Lumat3 LB9508 luminometer (Berthold Technologies, Bad Wildbad, Germany).

Qiu H., Zhong J., Luo L., Tang Z., Liu N., Kang K., Li L, & Gou D. (2017). Regulatory Axis of miR-195/497 and HMGA1-Id3 Governs Muscle Cell Proliferation and Differentiation. International Journal of Biological Sciences, 13(2), 157-166.

+ Open protocol

+ Expand

Chemiluminescence in Rat Brain Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ex vivo experiments were performed using 10 Wistar rats. Rats in the control group (n = 5) received i.p. injections of 20 µL of Tween 80 in 2 mL of isotonic NaCl solution. Rats in the experimental group (n = 5) received i.p. injections of a 10 mg/kg TRYP-Ox suspension. Rats in each group were decapitated 3 h after i.p. injections under diethyl ether anesthesia. The brains were removed, and the cortex of the left hemisphere (100 mg) was dissected and homogenized in 1 mL of ice-cold 0.05 M KH₂PO₄∙NaOH buffer, pH 7.4. Homogenization was performed using a 20 G needle 10 times and then with a 22 G needle 5 times. Next, 950 μL of buffer was added to 50 μL of the homogenate, and integral chemiluminescence during 60 s was measured using a Lumat3 LB9508 luminometer (Berthold Technologies, Bad Wildbad, Germany) before and after 3 h incubation in the dark at 22 ± 1 °C [43 (link)].

Plotnikov M.B., Chernysheva G.A., Smol’yakova V.I., Aliev O.I., Anishchenko A.M., Ulyakhina O.A., Trofimova E.S., Ligacheva A.A., Anfinogenova N.D., Osipenko A.N., Kovrizhina A.R., Khlebnikov A.I., Schepetkin I.A., Drozd A.G., Plotnikov E.V., Atochin D.N, & Quinn M.T. (2023). Neuroprotective Effects of Tryptanthrin-6-Oxime in a Rat Model of Transient Focal Cerebral Ischemia. Pharmaceuticals, 16(8), 1057.

+ Open protocol

+ Expand

Cell-Free Translation of GLuc mRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell free translation of GLuc mRNA was performed using Rabbit Reticulocyte Lysate System, Nuclease Treated (Promega). After 4 h of the incubation of GLuc mRNA (50 ng) in rabbit reticulocyte lysate (14 μL), GLuc expression level was measured using Renilla Luciferase Assay System (Promega) and Lumat³ LB9508 luminometer (Berthold Technologies, Bad Wildbad, Germany).

Kurimoto S., Yoshinaga N., Igarashi K., Matsumoto Y., Cabral H, & Uchida S. (2019). PEG-OligoRNA Hybridization of mRNA for Developing Sterically Stable Lipid Nanoparticles toward In Vivo Administration. Molecules, 24(7), 1303.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!