Rat egf

Manufactured by Thermo Fisher Scientific

Sourced in United States

Rat EGF is a recombinant protein that represents the epidermal growth factor (EGF) derived from the rat. EGF is a small protein involved in the regulation of cell growth and proliferation.

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Lab products found in correlation

Dmem f12, by Merck Group (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) Insulin, by Merck Group (1 mentions) Human serum, by Thermo Fisher Scientific (1 mentions) Evos microscope, by Thermo Fisher Scientific (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Nadp nadph quantification kit, by Merck Group (1 mentions) In situ brdu red dna fragmentation tunel assay kit, by Abcam (1 mentions) U 13c6 glucose, by Cambridge Isotopes (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Carbachol, by Merck Group (1 mentions) Synthetic rvd1, by Cayman Chemical (1 mentions) Ag1478, by Bio-Techne (1 mentions) Rpmi 1640 media, by Lonza (1 mentions)

Spelling variants of this product

Recombinant rat EGF (2 citations)

3 protocols using rat egf

Conjunctival Tissue Culture Protocol

Cited 1 time

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To define the impact of extracellular matrix hydrogels on gel-forming mucin secretion, we used a previously published method to obtain a co-cultivation of stratified squamous cells, goblet cells, and undifferentiated/stem cells [56 (link)]; starting with mincing the isolated conjunctival tissue to maintaining the cultures as described in 4.2.2. The medium was composed of Dulbecco’s Modified Eagle Medium (DMEM)/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 100 µg/mL penicillin/streptomycin (Lonza, Basel, Switzerland), 1 µg/mL insulin (Sigma-Aldrich, St. Louis, MO, USA), 0.5 µg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 2 ng/mL rat EGF (PeproTech, Cranbury, NJ, USA), and 10% human serum (Thermo Fisher Scientific, Waltham, MA, USA). The explant conjunctival cultures were photographed using an EVOS microscope (Thermo Fisher Scientific, Waltham, MA, USA).

Van Acker S.I., Van den Bogerd B., Van Acker Z.P., Vailionytė A., Haagdorens M., Koppen C., Ní Dhubhghaill S., Dartt D.A, & Pintelon I. (2021). Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures. International Journal of Molecular Sciences, 22(19), 10528.

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Halofuginone Cytotoxicity and Apoptosis Assay

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Halofuginone hydrobromide, 2′, 7′-dichlorodihydrofluorescin diacetate (DCFH-DA), Collagenase A, Dnase I, HPLC-grade acetonitrile, HPLC-grade methanol, HPLC-grade isopropanol, Methyl tertbutyl ether (MTBE), Ammonium acetate, N-Acetyl-L-cysteine, acetic acid, Dulbecco's Modified Eagle's Medium without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate (DMEM, D5030) and Pierce (R) BCA Protein Assay Kit and [NADP⁺]/[NADPH] Quantification Kit were obtained from Sigma-Aldrich (Munich, Germany). Rat EGF was purchased from Pepro Tech (Rocky Hill, USA). [U-¹³C₆]-glucose was purchased from Cambridge Isotope Laboratories (Tewksbury, USA). FITC Annexin V Apoptosis Detection Kit I was obtained from BD Bioscience (USA). Dialyzed Fetal Bovine Serum (US Origin SH30079.03) was purchased from HyClone (USA). In situ BrdU-red DNA fragmentation (TUNEL) assay kit (ab66110) was obtained from Abcam.

Chen G.Q., Tang C.F., Shi X.K., Lin C.Y., Fatima S., Pan X.H., Yang D.J., Zhang G., Lu A.P., Lin S.H, & Bian Z.X. (2015). Halofuginone inhibits colorectal cancer growth through suppression of Akt/mTORC1 signaling and glucose metabolism. Oncotarget, 6(27), 24148-24162.

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Resolvin Lipid Mediator Synthesis

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Synthetic RvD1 and RvE1 were purchased from Cayman Chemical, Ann Arbor, MI. Both compounds were dissolved in ethanol as supplied by the manufacturer and were stored at 80°C with minimal exposure to light. AG1478 was purchased from Tocris (Minneapolis, MN), TAPI 2 was purchased from EMD Biosciences (San Diego, CA). Rat EGF were purchased from Peprotech (Rocky Hill, NJ). Fura2/AM was purchased from Invitrogen (Carlsbad,CA). RPMI 1640 media was from Lonza (Walkersville, MD). siRNA and transfection reagents were purchased from Dharmacon (Lafayette, CO). Antibodies against phosphorylated (active) and total AKT were from Cell Signaling Technologies (Danvers, MA) while antibodies against phosphorylated (active) and total ERK 1/2 were from Santa Cruz Biotechnologies (Santa Cruz, CA). Carbachol was from Sigma-Aldrich (St. Louis, MO).
Immediately prior to use, the Rvs were diluted in Krebs-Ringer bicarbonate buffer with HEPES (KRB-HEPES, 119 mM NaCl, 4.8 mM KCl, 1.0 mM CaCl₂, 1.2 mM MgSO₄, 1.2 mM KH₂PO₄, 25 mM NaHCO₃, 10 mM HEPES, and 5.5 mM glucose [pH 7.45]) to the desired concentrations and added to the cells. The cells were incubated at 37 °C in the dark. Daily working stock dilutions were discarded following each experiment.

Kaye R., Botten N., Lippestad M., Li D., Hodges R.R., Utheim T.P., Serhan C.N, & Dartt D.A. (2018). Resolvin D1, but not Resolvin E1, Transactivates the Epidermal Growth Factor Receptor to Increase Intracellular Calcium and Glycoconjugate Secretion in Rat and Human Conjunctival Goblet Cells. Experimental eye research, 180, 53-62.

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