β actin

Manufactured by Dianova

β-actin is a structural protein found in all eukaryotic cells. It is commonly used as a reference or housekeeping gene in various molecular biology techniques, such as Western blotting and real-time PCR, to normalize and compare the expression levels of target genes across different samples.

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Lab products found in correlation

Ab31721, by Abcam (1 mentions)

2 protocols using β actin

Western Blot Analysis of Occludin

Cited 1 time

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Immunoreactivity for occludin (anti-occludin, ab31721, 1:5,000, Abcam) in the lesioned cortices was detected by Western blot analysis as previously described [4 (link)]. Densitometric analysis of occludin was performed in a blinded fashion using ImageJ software with β-actin (A5441, 1:500,000, Dianova) as loading control to normalize the levels of occludin detected.

Hopp S., Nolte M.W., Stetter C., Kleinschnitz C., Sirén A.L, & Albert-Weissenberger C. (2017). Alleviation of secondary brain injury, posttraumatic inflammation, and brain edema formation by inhibition of factor XIIa. Journal of Neuroinflammation, 14, 39.

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Quantifying GPIb Expression in Lesioned Cortices

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Immunoreactivity for GPIb (anti‐GPIb, 1:500, EMFRET Analytics) in lesioned cortices was detected by Western blot analysis as previously described.24 Densitometric analysis of GPIb was performed in a blinded way using ImageJ software with β‐actin (A5441, 1:500,000; Dianova) as loading control to normalize the levels of GPIb detected.

Hopp S., Albert‐Weissenberger C., Mencl S., Bieber M., Schuhmann M.K., Stetter C., Nieswandt B., Schmidt P.M., Monoranu C., Alafuzoff I., Marklund N., Nolte M.W., Sirén A, & Kleinschnitz C. (2016). Targeting coagulation factor XII as a novel therapeutic option in brain trauma. Annals of Neurology, 79(6), 970-982.

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