Approximately 100 mg wet liver tissue for each mouse was placed in 60°C incubator for 24 h to remove the water inside. The Hyp levels in the liver tissue (100 mg) were determined according to Hyp detection kit (Nanjing Jiancheng Bioengineering Institute, China). The Hyp content was expressed in micrograms of Hyp per milligram of dry weight (μg/mg) (Satoh et al., 2017 (link)).
Hyp detection kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Hyp detection kit is a laboratory tool designed to detect the presence of hypoxanthine (Hyp) in samples. Hyp is a purine metabolite that serves as an indicator of tissue hypoxia. The kit provides a straightforward and reliable method for Hyp quantification, enabling researchers to assess cellular oxygen levels in various experimental settings.
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Lab products found in correlation
6 protocols using hyp detection kit
1
Quantifying Liver Hydroxyproline Levels
2
Histopathological and Biochemical Assessment of Rat Lung Injury
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Briefly, the rat lung specimens were collected, fixed in 4% buffered paraformaldehyde for 24 h, embedded in paraffin, and sectioned to 5 µm-thick slices by freezing microtome. Next, the tissue sections were deparaffinized, hydrated, and stained with hematoxylin and eosin (H&E) (Beyotime, Shanghai, China) and the Modified Masson’s Trichrome stain kit (Solarbio, Beijing, China). The histological examination was performed by 2 independent pathologists blinded to the study protocol. The wet/dry (W/D) weight ratio was determined to assess the degree of pulmonary edema following PQ administration. In brief, the wet weight was measured after excising the lung tissues, and the lung was then placed in an oven until the weight remained constant, at which time it was weighed again to measure the dried weight. The W/D weight ratio was calculated by dividing the dry weight by the wet weight. A hydroxyproline (HYP) detection kit (Jiancheng, Nanjing, China) was used to measure the HYP content of rat lungs to assess the lung collagen deposition.
3
Serum Biomarkers and Tissue Hydroxyproline
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At the end of the experiment, animals were sacrificed by i.p. injection of 200 mg/kg pentobarbitone. Blood was collected and serum was separated by centrifugation at 3000 g for 5 min. Serum AST, ALT, and TBil were quantified by a biochemical autoanalyzer. The tissue hydroxyproline (HyP) level was examined with a HyP detection kit (Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer's instructions.
4
Measuring Lung Collagen Content
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The collagen content in the lung tissue was measured using the hydroxyproline (Hyp) assay as described previously (31 (link)). Hyp content was measured using a Hyp detection kit (Jiancheng Biotechnology Institute, Nanjing, China) according to the manufacturer’s instructions.
5
Quantifying Skin Collagen and Oxidative Markers
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Total skin collagen can be determined by evaluating the content of hydroxyproline (HYP), the major constituent amino acid in collagen [19 (link)]. HYP levels in the dorsal skin were measured using a HYP detection kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions. Malondialdehyde (MDA) [20 (link)] level and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity in skin tissue were determined using commercial kits (Nanjing Jiancheng Bioengineering Institute).
6
Collagen Quantification in Mouse Lungs
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The collagen content in whole mouse lungs was quantified by measuring hydroxyproline (HYP) content in pulverized left lungs as described previously (68 (link)). HYP content was measured using a HYP detection kit (Jiancheng Biotechnology Institute, Nanjing, China) according to the manufacturer's protocol.
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