Cortecs c18 column

Manufactured by Waters Corporation

Sourced in United States

The Cortecs C18 column is a high-performance liquid chromatography (HPLC) column designed for the analysis of a wide range of compounds. The column features a solid-core particle technology that provides efficient separation and faster analysis times. The C18 stationary phase offers selectivity for the retention and separation of both polar and non-polar analytes.

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Lab products found in correlation

Q exactive orbitrap mass spectrometer, by Thermo Fisher Scientific (4 mentions) Ultimate 3000 uhplc, by Thermo Fisher Scientific (3 mentions) Tracefindertm, by Thermo Fisher Scientific (2 mentions) Acquity uplc 1 class, by Waters Corporation (2 mentions) Acquity uhplc 1 class system, by Waters Corporation (2 mentions) Beh c18 column, by Waters Corporation (2 mentions) Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Compass data analysis software, by Bruker (1 mentions) High performance liquid chromatography (hplc), by Shimadzu (1 mentions) Simca v14, by Sartorius (1 mentions) Chemstation, by Hewlett-Packard (1 mentions) Hp1100 series, by Hewlett-Packard (1 mentions) Ltq xl, by Thermo Fisher Scientific (1 mentions) N4 coulter particle size analyzer, by Brookhaven Instruments (1 mentions) Zetaplus, by Brookhaven Instruments (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Diode array detector, by Agilent Technologies (1 mentions) Vanguard pre column, by Waters Corporation (1 mentions) 1100 series, by Agilent Technologies (1 mentions) Tracefinder 3, by Thermo Fisher Scientific (1 mentions)

49 protocols using cortecs c18 column

High-Resolution MS/MS Analysis of Peptides

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MS–MS analysis was performed on the peptides 3, 5, 6, and 7 using a Bruker Impact II Ultra-High Resolution QQ-Time-Of-Flight instrument (Bruker, Billerica, MA) with a CaptiveSpray electrospray ionization (ESI) source. The starting collision energy was 10 eV. The calibration error of the instrument was <0.5 ppm. Direct infusion was used on the samples DRVYIH and DRIYVH at a flow rate of 60 μL/h. To show peptide sequence, increasing collision energies of 10, 40, 50, and 60 eV were used. UHPLC–MS2 was employed for 3 and 7. UHPLC was performed using a Shimadzu HPLC (Shimadzu Corp., Kyoto, Japan) Nexera UHPLC system. Separation was carried out with a Cortecs C18 column (10 cm × 3 mm I.D., 2.7 μm particle size, Waters Corp., Milford, MA) with a linear gradient of 5% ACN with 0.1% formic acid in water (solution A) and 100% ACN with 0.1% formic acid (solution B) over 25 min at a flow rate of 0.8 mL/min. Analysis was performed using Bruker Compass Data Analysis software (v4.4).

Choules M.P., Bisson J., Gao W., Lankin D.C., McAlpine J.B., Niemitz M., Jaki B.U., Franzblau S.G, & Pauli G.F. (2019). Quality Control of Therapeutic Peptides by 1H NMR HiFSA Sequencing. The Journal of organic chemistry, 84(6), 3055-3073.

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BALF Metabolomic and Lipidomic Analysis

Cited 2 times

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The detailed method of BALF sample pretreatment is described in the

Supplement 3

. All compounds in BALF were analyzed using a Cortecs C18 column (2.1 × 100 mm, Waters) on an Ultimate 3000 UHPLC (Dionex) system coupled with a Q Exactive (Orbitrap) mass spectrometer (Thermo Fisher, CA). Detailed parameters for the untargeted metabolic and lipidomic analyses were set following the protocols of our previously reported study.23 (link) Data-dependent MS/MS acquisition (DDA) of all samples was analyzed using TraceFinderTM (Thermo, CA). Metabolites and lipids were identified based on matching precursor and characteristic fragment masses and then assigned using in-house databases in “screening” mode. Any metabolite feature with more than 20% missing values was removed from the result.24 (link),25 (link) Missing values were estimated by the Bayesian PCA (BPCA) method. Data were normalized by the QC group and then auto-scaled using MetaboAnalystR 3.0.26 (link) The normalized data were used for downstream analysis. Principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) were conducted using SIMCA v14.1 (Umetrics, Sweden).

He Y., Yu W., Ning P., Luo Q., Zhao L., Xie Y., Yu Y., Ma X., Chen L., Zheng Y, & Gao Z. (2022). Shared and Specific Lung Microbiota with Metabolic Profiles in Bronchoalveolar Lavage Fluid Between Infectious and Inflammatory Respiratory Diseases. Journal of Inflammation Research, 15, 187-198.

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Chromatographic Analysis of Butyl Benzoate

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The chromatographic experiments were carried out using an HP 1100 Series liquid chromatograph (Hewlett Packard, now Agilent Technologies, Palo Alto, CA, USA), equipped with a multisolvent delivery system, an automatic injector, a column thermostat a DAD detector, and an HP Chemstation data aquisition system. Band profiles of butyl benzoate were recorded at 290 nm.
The column used during the experiments was a 50 mm × 2.1 mm Waters Cortecs C18 column packed with 5 μm particles. 65:35 methanol-water mixture was used as the eluent.

Horváth S., Lukács D., Farsang E, & Horváth K. (2023). Unbiased Determination of Adsorption Isotherms by Inverse Method in Liquid Chromatography. Molecules, 28(3), 1031.

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Quantitative Assessment of Phenolic Compounds

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Quantitative assessment of phenolic compounds was carried out through HPLC–MS (LTQ XL, Thermo Fisher Scientific, San Jose, CA, USA) analysis (Świeca et al. 2012 (link)). The HPLC–MS system was equipped with a ternary pump, auto sampler, and thermostatic column compartment, diode array detector (Surveyor, Thermo Fisher), and a linear ion trap mass spectrometer (LTQ XL, Thermo Fisher Scientific, San Jose, CA, USA) equipped with an electrospray ionization (ESI) source. A CORTECS C18 column (2.1 mm × 100 mm, 2.6 µm; Waters) was used; the column temperature was maintained at 35 °C. The mobile phase A (0.1% formic acid/water) and B (acetonitrile) was used, the gradient program was as follows: 0–2 min 5.0% B; 4–11 min 15–35% B; 15–17 min, 100% B; 17.5–22 min, 5.0% B; flow rate was 0.25 mL min⁻¹, the injection volume was 4 μL. UV detection was performed at 270 and 370 nm, the wavelength was scanned from 200–600 nm. MS was scanned in ESI source in negative mode, mass range: m/z 92–1000; source voltage was 3.5 kV, capillary temperature was 350 °C, sheath gas flow was 35, aux gas flow was 15.0, sweep gas flow was 1.0, and capillary voltage was 43 V. Data acquisition, handling, and instrument control were performed using Xcalibur 2.3.1 software.

Khalid M., Hassani D., Bilal M., Asad F, & Huang D. (2017). Influence of bio-fertilizer containing beneficial fungi and rhizospheric bacteria on health promoting compounds and antioxidant activity of Spinacia oleracea L.. Botanical Studies, 58, 35.

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Liposomal Doxorubicin Characterization

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Particle size and zeta potential analysis was carried out using an N4 Coulter particle size analyzer (West Lafayette, IN) and Zetaplus (Brookhaven Instruments Corporation, Holtsville, NY) respectively. For particle size analysis, 5μL of the liposomal solution was mixed with 990μL of 1mM KCl while for zeta potential, 50μL was mixed with 1.5mL of 1mM KCl. For the determination of liposomal Dox content, the liposomes were dissolved in methanol at a dilution factor of 50 and the absorbance was measured at 480 nm. The drug concentration was determined by comparison with a standard curve of free Dox in methanol (0–60 μg/mL). To characterize the pH-dependent release of Dox from the liposomes, 0.8 mL of the liposomal Dox solution was dialyzed against 40 mL of either 300mM citrate buffer pH 5.0 or PBS pH 7.4 using a MWCO membrane of 12–14kDa for 96 hours. The release samples were taken at the mentioned time points while being replaced with equal volumes of fresh buffer and the Dox content in the release buffer was determined using HPLC (Hitachi Elite LaChrom, Pleasanton, CA). For the HPLC method, a Waters (Milford, MA) Cortecs^™ C18 column (2.7 μm, 4.6 mm × 150 mm) was used with a mobile phase of 60% (v/v) acetonitrile and 40% (v/v) of 0.1% trifluoroacetic acid in water with a flow rate of 1 mL/min. The Dox peak was determined by fluorescence (Ex/Em 445/550 nm).

Sriraman S.K., Salzano G., Sarisozen C, & Torchilin V. (2016). Anti-Cancer Activity of Doxorubicin-Loaded Liposomes Co-Modified with Transferrin and Folic Acid. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 105, 40-49.

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Pharmacokinetics of Retinoid IRX4647

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Inbred 8–12 weeks old female C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME) were housed in ventilated cages on a regular 12 h light/dark cycle with ad libitum access to food and water. Animals acclimated for at least one week before a single dose administration of the studied retinoid via oral gavage. Compound IRX4647 was formulated in 2.5% DMSO/30% PEG400/67.5% Phosal MCT53 solution. Plasma and liver from compound-treated mice (n = 5 per time point) were individually harvested at 0.5, 1, 3, 6, 12, 24, and 48 h post-drug administration. Samples were flash frozen and stored at − 80 °C until analysis. Plasma was isolated as were liver homogenates and samples were extracted with acetonitrile and 0.1% formic acid for 30 min at room temperature. Extracts were separated by centrifugation and used for high-performance liquid chromatography (HPLC) analysis. HPLC analyses were performed with a Shimadzu 20AC-XR system. Separation was at room temperature with a Cortecs C18 column (Waters Corp., Milford, MA).

Wei C.H., Huang L., Kreh B., Liu X., Tyutyunyk-Massey L., Kawakami M., Chen Z., Shi M., Kozlov S., Chan K.C., Andresson T., Carrington M., Vuligonda V., Sanders M.E., Horowitz A., Hwu P., Peng W., Dmitrovsky E, & Liu X. (2023). A novel retinoic acid receptor-γ agonist antagonizes immune checkpoint resistance in lung cancers by altering the tumor immune microenvironment. Scientific Reports, 13, 14907.

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Chromatographic Analysis of Sulfur Compounds

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Sulfur compounds were detected according to the method described by Tomšik et al. (2018) [8 (link)]. Liquid chromatograph (Agilent 1100 series, Paolo Alto, CA, USA), using CORTECS C18 column (internal diameter 100 × 100.6 mm, charge size 2.7 mm), with Waters VanGuard pre-column (internal diameter 5 × 3.9 mm; charge size 2.7 mm) and diode array detector (Agilent, USA) in stationary mode were used to determine sulfur compounds. The solvent flow was adjusted to 1 mL/min, and the injection volume was 50 µL. A mixture of phosphate buffer (20 mM, pH 4.5), 95% and acetonitrile 5% was used as the mobile phase. Chromatograms were recorded at a wavelength of 210 nm. Quantification was determined by integrating the obtained peaks and comparing the results with a series of standards of known concentrations by creating calibration curves.

Stupar A., Šarić L., Vidović S., Bajić A., Kolarov V, & Šarić B. (2022). Antibacterial Potential of Allium ursinum Extract Prepared by the Green Extraction Method. Microorganisms, 10(7), 1358.

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Quantifying Methylated Cardiolipin Lipids

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Cortecs C18 column (2.1 × 100 mm, Waters) was applied in the analysis. Mobile phase A was made by mixing 400 mL of HPLC-grade water containing 0.77 g of ammonium acetate with 600 mL of HPLC-grade acetonitrile. Mobile phase B contained 10% acetonitrile and 90% isopropanol (v/v). The gradient was as follows: 0 min, 40% B; 3.0 min, 40% B; 23 min, 98% B; 30 min, 98% B; 30.5 min, 40% B; 35 min, 40% B. Data were acquired using Q Exactive orbitrap mass spectrometer (QEHF, Thermo Fisher, USA). Resolution of 60,000 and 30,000 was used for MS and MS/MS acquisition. The detailed mass spectrometer parameters are as follows: spray voltage, 3.2 kV for positive; capillary temperature, 320 °C; aux gas flow rate (arb),10; mass range (m/z), 240–2000 for positive mode. Lipids were identified and quantified using Tracefinder 3.2 (Thermo Fisher, USA). In-house database containing 780 methylated CL molecules, which is compatible with Tracerfinder, was applied for lipid identification.

Wang X., Lin X., Zheng Z., Lu B., Wang J., Tan A.H., Zhao M., Loh J.T., Ng S.W., Chen Q., Xiao F., Huang E., Ko K.H., Huang Z., Li J., Kok K.H., Lu G., Liu X., Lam K.P., Liu W., Zhang Y., Yuen K.Y., Mak T.W, & Lu L. (2021). Host-derived lipids orchestrate pulmonary γδ T cell response to provide early protection against influenza virus infection. Nature Communications, 12, 1914.

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Lipid Analysis in Milk using LC-MS/MS

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Lipids were extracted from milk as described (28 (link), 29 (link)). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for lipid analysis have been previously described (30 (link), 31 (link)). Briefly, reverse phase chromatography was selected for LC separation using Cortecs C18 column (2.1 × 100 mm, Waters). Mobile phase A was made by mixing 400 ml of HPLC-grade water containing 0.77 g of ammonium acetate with 600 ml of HPLC-grade acetonitrile (pH ∼ 7). Mobile phase B contained 10% acetonitrile and 90% isopropanol (v/v). Data were acquired using QExactive orbitrap mass spectrometer (Thermo Fisher Scientific, CA) coupled with UHPLC system Ultimate 3000 (Thermo Fisher Scientific, CA).

Li L., Zhou H., Wang J., Li J., Lyu X., Wang W., Luo C., Huang H., Zhou D., Chen X., Xu L, & Li P. (2023). Metabolic switch from glycogen to lipid in the liver maintains glucose homeostasis in neonatal mice. Journal of Lipid Research, 64(10), 100440.

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Targeted LC-MS/MS Analysis of Homeostatic Lipids

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We used a Xevo TQ-S mass spectrometer equipped with Acquity I Class UPLC (Waters Corporation, Milford, MA). CORTECS C18 column (2.7 µm particle size, 4.6 mm

\times

100 mm i.d.) (Waters Corporation, #186007377) for the separation of fatty acids and their derivatives. LC conditions are as follow; 45% of solvent A (H₂O + 0.01% acetic acid) and 55% of solvent B (MeOH + 0.01% acetic acid) with a flow rate of 0.6 mL/min was initially set at 0 min., then gradient was set for 85% B at 10 min, 98% B at 18 min, 100% B at 20 min, back to 55% B at 30 min. The capillary voltage was −2.5 kV, desolvation temperature was 600 °C, desolvation gas flow was 1100 L/h, cone gas was 150 L/h, and nebulizer pressure was 7.0 bar with a source temperature of 150 °C. MassLynx ver. 4.1 software (Waters Corporation) was used for the operation and recording of the data. Data were expressed as the change in the relative abundance of pro-homeostatic lipid mediators normalized with the internal standards.

Miyagishima K.J., Sharma R., Nimmagadda M., Clore-Gronenborn K., Qureshy Z., Ortolan D., Bose D., Farnoodian M., Zhang C., Fausey A., Sergeev Y.V., Abu-Asab M., Jun B., Do K.V., Kautzman Guerin M.A., Calandria J., George A., Guan B., Wan Q., Sharp R.C., Cukras C., Sieving P.A., Hufnagel R.B., Bazan N.G., Boesze-Battaglia K., Miller S, & Bharti K. (2021). AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration. Communications Biology, 4, 1360.

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