Rtx 5ms fused bonded column

CLLEO was analysed using Shimadzu GCMS-QP2010 (Kyoto, Japan) fitted with Rtx-5MS fused bonded column (30 m × 0.25 mm i.d. × 0.25 µm film thickness; Restek, PA, USA) equipped with a split–splitless injector. The column temperature was initially kept at 45 °C for 2 min (isothermal) and raised to 300 °C at a rate of 5 °C/min then held at 300 °C for 5 min (isothermal). The injection temperature was 250 °C. Helium carrier gas was used at a flow rate of 1.41 mL/min. The mass spectrometer was scanned over the 35 to 500 m/z with an ionizing voltage of 70 eV; a filament emission current of 60 mA and an ion source temperature 200 °C. A 1 µL of the diluted sample (1% v/v) was injected with split mode (split ratio 1:15). The identification of CLLEO components was based on the comparison of their retention indices (RI) and mass spectra (MS) to that reported in Adams library [35 ], NIST 11 Mass Spectral Library (NIST11/2011/EPA/NIH), Wiley library database 10th edition and the literature data [36 (link),37 (link),38 (link),39 (link),40 (link),41 (link),42 (link),43 (link),44 (link)]. The retention indices were assigned in relation to those of a homologous set of standard n-alkanes (C₈–C₂₈) injected under the same conditions. The identified compounds and their percentages are listed in Table 1.

GC/MS analysis was performed using Shimadzu GC/MS QP-2010 equipped with Rtx-5MS fused bonded column (30 m × 0.25 mm i.d. × 0.25 µm film thickness) (Restek, USA). The used conditions were as previously described³¹ . Volatile components were identified by direct comparison of their retention indices (RI) and mass spectral data with NIST Mass Spectral Library and literature^{18 ,32 (link)–36 (link)}. The relative content of each peak was calculated based on the percentage of peak area relative to the total peak area. RI was calculated relative to a homologous series of n-alkanes (C8-C28) injected under the same conditions^{37 (link)}.

Mass spectra were recorded using Shimadzu GCMS-QP2010 (Tokyo, Japan) equipped with a Rtx-5MS fused bonded column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) (Restek, Bellefonte, PA, USA) equipped with a split–splitless injector. The capillary column was coupled to a quadrupole mass spectrometer (SSQ 7000; Thermo-Finnigan, Bremen, Germany). The initial column temperature was held at 45 °C for 2 min (isothermal) and programmed to 300 °C at a rate of 5 °C/min and kept constant at 300 °C for 5 min (isothermal). The injector temperature was 250 °C. The helium carrier gas flow rate was 1.41 mL/min. All the mass spectra were recorded applying the following conditions: (equipment current) filament emission current, 60 mA; ionization voltage, 70 eV; ion source, 200 °C. Diluted samples (1% v/v) were injected with split mode (split ratio 1: 15). The sample (1 μL) was injected automatically into the chromatograph using an AOC-20i auto-sampler. Volatile components were deconvoluted using AMDIS software (www.amdis.net) and identified by its mass spectrum matching to the database and with authentic standards (when available) [30 ].