Quantitative assessment of apoptosis of HaCaT cells was analyzed by anti-human annexin V (BioLegend, San Diego, CA, USA) using propidium iodide solution (PI) staining. Briefly, cells grown in p60 tissue culture plates for 24 h with HT at 1, 5, and 10 µM, H2O2 or combined as indicated, were harvested with trypsin, washed in phosphate buffer saline (PBS), and subjected to apoptosis determination by the procedure described in detail elsewhere [70 (link)].
Anti human annexin 5
Manufactured by BioLegend
Sourced in United States
Anti-human Annexin V is a protein that binds to phosphatidylserine, a component of the cell membrane. This binding property is used to detect and quantify apoptotic cells.
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Lab products found in correlation
4 protocols using anti human annexin 5
1
Apoptosis Quantification of HaCaT Cells
2
Quantitative Apoptosis Analysis of U251 Glioma Cells
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Quantitative assessment of apoptosis of U251 glioma cell lines was analyzed by anti-human Annexin V (BioLegend, San Diego, CA, USA) and PI staining. Briefly, cells grown in 100-mm dishes for 72 h in EMEM containing 2% FBS were harvested with trypsin and washed in PBS. The cells were resuspended in Annexin V binding buffer (10 mM HEPES/NaOH, pH 7; 140 mM NaCl; and 2.5 mM CaCl2) and stained with Annexin V-FITC for 20 min at room temperature (RT) and then with PI at RT for additional 15 min in the dark. The cells were acquired by flow cytometer within 1 h after staining. At least 10, 000 events were collected, and the data were analyzed by Cell- Quest Pro software (Becton Dickinson, San Jose, CA). Data are expressed as logarithmic values of fluorescence intensity.
3
Quantitative Apoptosis Assay of HaCaT Cells
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Quantitative assessment of apoptosis of HaCaT cells was analysed by antihuman annexin V (BioLegend, San Diego, CA, USA) using propidium iodide solution (PI) staining. Briefly, cells grown in 100-mm dishes for 24 h with G. lucidum extracts, H2O2 or combined as indicated were harvested with trypsin, washed in phosphate buffer saline (PBS) and subjected to apoptosis determination by the procedure described in detail elsewhere [43 (link)].
4
Apoptosis Evaluation of Glioma Cells
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The evaluation of the apoptosis of U87MG glioma cell lines, NHA and patient-derived primary cell line (GBM39) was conducted by anti-human Annexin V (BioLegend, San Diego, CA, USA) and PI staining. Briefly, cells grown in 100-mm dishes for 48 h in EMEM, ABM or DMEMF12 supplemented were harvested with trypsin and washed in PBS. The cells were resuspended in Annexin V binding buffer (10 mM HEPES/NaOH, pH 7; 140 mM NaCl; and 2.5 mM CaCl2) and stained with Annexin V-FITC for 20 min at room temperature (RT) and then with PI at RT for additional 15 min in the dark. The cells were acquired by flow cytometer within 1 h after staining. At least 10,000 events were collected, and the data were analyzed by CellQuest Pro software (Becton Dickinson, San Jose, CA). Data are expressed as logarithmic values of fluorescence intensity.
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