Embryos and larvae were fixed with AB fix solution (8% PFA and 2× fix solution [8% sucrose and 0.03% 1 M CaCl2 in PBS]) overnight, embedded in 1.5% agarose dissolved in 5% sucrose, and kept in a sealed 15-ml tube with 30% sucrose in PBS overnight. Subsequently, agarose blocks were frozen in butanol (Junsei Chemical) chilled with liquid nitrogen and then serially sectioned into slices using a CM3000 S microtome (Leica).
Butanol
Manufactured by Junsei
Sourced in Japan
Butanol is a type of alcohol that can be used as a laboratory solvent or in the synthesis of other chemicals. It has a specific chemical structure and physical properties that make it suitable for certain applications in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Hexane, by Junsei (2 mentions)
Ethyl acetate, by Junsei (2 mentions)
Methanol, by Junsei (2 mentions)
Titanium isopropoxide, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Trimesic acid, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Kanto Chemical (1 mentions)
Hydrochloric acid (hcl), by Kanto Chemical (1 mentions)
Cell lysis buffer, by Merck Group (1 mentions)
Palmitic acid, by Fujifilm (1 mentions)
Dimethyl sulfoxide (dmso), by Kanto Chemical (1 mentions)
Gallic acid, by Kanto Chemical (1 mentions)
Rutin, by Kanto Chemical (1 mentions)
Linoleic acid, by Kanto Chemical (1 mentions)
Starch, by Merck Group (1 mentions)
Tyrosinase, by Merck Group (1 mentions)
Sodium carbonate, by Kanto Chemical (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid, by Kanto Chemical (1 mentions)
Trichloroacetic acid, by Kanto Chemical (1 mentions)
β carotene, by Kanto Chemical (1 mentions)
4 protocols using butanol
1
Embryo and Larval Fixation and Sectioning
2
Synthesis of Titanium-Based Metal-Organic Frameworks
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All chemical agents were obtained from manufacturers and used as obtained without further purification. The following chemicals were used in the present study. Titanium isopropoxide: 97%, Alfa Aesar; titanium-diisopropoxide bis (acetyacetonate): 75%, Sigma Aldrich; H2SO4: 97%, Matsuneon Chemicals Ltd.; trimesic acid: 98%, Alfa Aesar; Cu(NO3)2.3H2O: 99%, Dae Jung Reagent Chemicals; butanol: 99%, Junsei Chemical Co., Ltd; N,N-dimethylformamide (i.e. DMF): 99.8%, Macron Chemicals; acetonitrile: 99%, Burdick & Jackson, and iodine (i.e., I2): Duksan Chemicals. Multi-walled carbon nanotubes (MWCNTs: CM-100) was purchased from Hanhwa Nanorech, Corp., and fluorine doped tin oxide (FTO, 8 ohm/sq, 77% transmittance in visible range) were obtained from Pilkington TEC Glass.
3
Bioactive Compound Extraction and Evaluation
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Extraction solvents including methanol, hexane, ethyl acetate, and butanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. Standards including gallic acid, rutin, butylated hydroxytoluene (BHT), kojic acid were purchased from Kanto Chemical Co., Inc., Tokyo, Japan. Palmitic acid was obtained from FUJIFILM Wako Pure Chemical Corporation.
Chemicals including sodium hypochlorite (NaClO), Folin-Ciocalteu’s reagent, sodium carbonate (Na2CO3), aluminum chloride (AlCl3), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate (K2S2O8), 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium acetate (CH3COONa), β-carotene, chloroform, linoleic acid, tween 40, dipotassium phosphate (K2HPO4), monopotassium phosphate (KH2PO4), potassium ferricyanide (K3[Fe(CN)6]), trichloro acetic acid (CCl3COOH), ferric chloride (FeCl3), dimethyl sulfoxide (DMSO), monosodium phosphate (NaH2PO4), sodium chloride (NaCl), hydrochloric acid (HCl) were obtained from Kanto Chemical Co., Inc., Tokyo, Japan. While tyrosinase, L-tyrosine, α-amylase, starch, iodine, 3-(4,5-dimethylthiazolyl)2,5-diphenyl-tetrazolium bromide (MTT), Iscove’s Modified Dulbecco’s Medium (IMDM), and cell lysis buffer were purchased from Sigma-Aldrich, St. Louis, MO, USA.
K562 and Meg-01 cell lines were obtained from American Type Culture Collection (ATCC), Virginia, United States.
Chemicals including sodium hypochlorite (NaClO), Folin-Ciocalteu’s reagent, sodium carbonate (Na2CO3), aluminum chloride (AlCl3), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate (K2S2O8), 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium acetate (CH3COONa), β-carotene, chloroform, linoleic acid, tween 40, dipotassium phosphate (K2HPO4), monopotassium phosphate (KH2PO4), potassium ferricyanide (K3[Fe(CN)6]), trichloro acetic acid (CCl3COOH), ferric chloride (FeCl3), dimethyl sulfoxide (DMSO), monosodium phosphate (NaH2PO4), sodium chloride (NaCl), hydrochloric acid (HCl) were obtained from Kanto Chemical Co., Inc., Tokyo, Japan. While tyrosinase, L-tyrosine, α-amylase, starch, iodine, 3-(4,5-dimethylthiazolyl)2,5-diphenyl-tetrazolium bromide (MTT), Iscove’s Modified Dulbecco’s Medium (IMDM), and cell lysis buffer were purchased from Sigma-Aldrich, St. Louis, MO, USA.
K562 and Meg-01 cell lines were obtained from American Type Culture Collection (ATCC), Virginia, United States.
4
Antioxidant Evaluation of C. tramdenum Bark
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Stem bark of C. tramdenum (TDB) was collected at 21.07°N, 106.10°E, Bac Ninh province, Vietnam in 2016. The species was identified at a study field based on Vietnam Plant Data Center (http://www.botanyvn.com ) and Plants Database Missouri Botanical Garden, United States (TROPICOS—http://www.tropicos.org ). The specimen with voucher number TDB-J2016 was deposited at the Plant Physiology and Biochemistry Laboratory, IDEC, Hiroshima University, Japan.
All extraction solvents including methanol, hexane, ethyl acetate, and butanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. The analytical reagents for antioxidant assays were acquired from Kanto Chemical Co., Inc., Tokyo, Japan while those of enzymatic assays were procured from Sigma-Aldrich, St. Louis, MO, USA.
A vacuum evaporator (Rotavapor® R-300, Nihon Buchi K.K., Tokyo, Japan), a microplate reader (MultiskanTM Microplate Spectrophotometer, Thermo Fisher Scientific, Osaka, Japan), and GC-MS system (JMS-T100 GCV, JEOL Ltd., Tokyo, Japan) were the main instruments used in this study.
All extraction solvents including methanol, hexane, ethyl acetate, and butanol were purchased from Junsei Chemical Co., Ltd., Tokyo, Japan. The analytical reagents for antioxidant assays were acquired from Kanto Chemical Co., Inc., Tokyo, Japan while those of enzymatic assays were procured from Sigma-Aldrich, St. Louis, MO, USA.
A vacuum evaporator (Rotavapor® R-300, Nihon Buchi K.K., Tokyo, Japan), a microplate reader (MultiskanTM Microplate Spectrophotometer, Thermo Fisher Scientific, Osaka, Japan), and GC-MS system (JMS-T100 GCV, JEOL Ltd., Tokyo, Japan) were the main instruments used in this study.
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