T4 polynucleotide kinase (3’-phosphatase minus, M0236) was obtained from New England Biolabs (NEB). 3’-phosphoadenosine 5’-phosphate and adenosine 3’-monophosphate were from Sigma-Aldrich. 100 μl reactions containing 100 mM Bis-tris propane pH 7.0, 1 mM DTT, 1mM EDTA, 200 nM coelenterazine sulfate, 10 nM of RLuc, and 1–20 nmoles of Coel-ST were performed at 25°C. Each reaction was initiated with the addition of the PAP sample and was followed by light emission. Relative light units per second (RLU/s) were measured in a Centro LB 960 luminometer (Berthold) plate reader.
3 phosphoadenosine 5 phosphate
Manufactured by Merck Group
Sourced in Czechia
3'-phosphoadenosine 5'-phosphate is a chemical compound that functions as a cofactor in various enzymatic reactions. It is involved in the regulation of cellular processes, including signal transduction and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine 3 monophosphate, by Merck Group (1 mentions)
Centro lb 960 luminometer, by Berthold Technologies (1 mentions)
Resorufin, by Merck Group (1 mentions)
Midazolam, by Merck Group (1 mentions)
Ethoxyresorufin, by Merck Group (1 mentions)
Chlorzoxazone, by Merck Group (1 mentions)
Benzyloxyresorufin, by Merck Group (1 mentions)
Bufuralol, by Merck Group (1 mentions)
Menadione, by Merck Group (1 mentions)
1 chloro 2 4 dinitrobenzene, by Merck Group (1 mentions)
Acenaphthenol, by Merck Group (1 mentions)
S mephenytoin, by Merck Group (1 mentions)
Udp glucuronic acid, by Merck Group (1 mentions)
7 ethoxy 4 trifluoromethylcoumarin, by Merck Group (1 mentions)
Cis nerolidol, by Merck Group (1 mentions)
Trans nerolidol, by Merck Group (1 mentions)
P nitrophenol, by Merck Group (1 mentions)
Testosterone, by Merck Group (1 mentions)
Diclofenac, by Merck Group (1 mentions)
Cytochrome c, by Merck Group (1 mentions)
2 protocols using 3 phosphoadenosine 5 phosphate
1
Bioluminescent Coelenterazine Kinetics
2
Sesquiterpenes and Xenobiotic Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sesquiterpenes cis-nerolidol (CNER), trans-nerolidol (TNER), farnesol (FAR), and other chemicals benzyloxyresorufin, ethoxyresorufin, resorufin, coumarin, 7-ethoxy-4-(trifluoromethyl)coumarin, S-mephenytoin, diclofenac, bufuralol, testosterone, midazolam, chlorzoxazone, menadione, 1-chloro-2,4-dinitrobenzene, p-nitrophenol, p-nitrophenylsulphate, 2-napthol, 3’-phosphoadenosine-5’-phosphate, acenaphthenol, reduced glutathione, UDP-glucuronic acid, cytochrome c, NADH, NADP and NADPH, were purchased from Sigma-Aldrich (Prague, Czech Republic). All other chemicals used were of HPLC or analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!