Immunohistochemistry was performed on formalin-fixed, paraffin-embedded (FFPE) tissue sections using the avidin–biotin complex method as described previously [16 (link)]. We used 4-μm-thick serial sections of each FFPE blocks with antibodies against the following: CD3 (PS1; 1:100) from Santa Cruz Biotechnology (Santa Cruz, CA, USA), CD4 (368; 1:100), and CD8 (4B11; 1:200) from Leica Microsystems (Newcastle-upon-Tyne, UK), and FOXP3 (42; 1:100) produced in-house [4 (link)]. Immunohistochemistry without the primary antibody was performed as a negative control.
Cd4 368
Manufactured by Leica
Sourced in United States
The CD4 (368) is a laboratory equipment product. It is used for the detection and quantification of CD4 T cells. The CD4 (368) provides accurate and reliable measurements of CD4 T cell counts.
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Lab products found in correlation
2 protocols using cd4 368
1
Immunohistochemistry of FFPE Tissues
2
Immunohistochemical Analysis of Immune Markers
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Immunohistochemistry was carried out on formalin‐fixed, paraffin‐embedded tissue sections using the avidin–biotin complex method as described previously.41 We used 4‐μm‐thick serial sections of representative blocks with antibodies against the following: CD3 (PS1; 1:100) from Santa Cruz Biotechnology (Santa Cruz, CA, USA), CD4 (368; 1:100) and CD8 (4B11; 1:200) from Leica Microsystems (Newcastle‐upon‐Tyne, UK), FOXP3 (42; 1:100) produced in house,18 BTLA (HPA047211; 1:500) from Atlas Antibodies (Stockholm, Sweden), and Cbl‐b (246C5A; 1:50) from Abcam (Cambridge, UK). Immunohistochemistry without the primary antibody was used as a negative control.
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