Alpha streptavidin donor beads

The following item was purchased from ATCC: Vero-E6 (CRL-1586, RRID:CVCL_0574). The following items were purchased from Corning TM: EMEM (10–009-CV), HI FBS (35–016-VC) and 0.25% Trypsin (25053CI). The untagged NP (Z03501) was purchased from Genscript. The His-tagged NP (40588-V08B) was purchased from SinoBiological.
The following item was purchased from Gibco: Pen/Strep (15140–122). PBS (SH30256FS) was purchased from HyClone. The following items were purchased from PerkinElmer: ProxiPlate-384 Plus (Cat# 6008280), CulturPlate-384 (Cat#: 6007680), Alpha Streptavidin Donor beads (6760002), AlphaLISA lysis buffer (AL003C). The following custom labeling was performed by PerkinElmer: donor antibodies were biotinylated using NHS activated biotinylating reagent (ChromaLink #B-1001–105), and acceptor antibodies were conjugated to Alpha Acceptor Beads (PerkinElmer #6760137M).

S3 Table in S1 File contains detailed information about the sources of the recombinant enzymes. Peptide substrates were from AnaSpec (Fremont, CA). α-Ketoglutarate (disodium salt dihydrate, 75892), L-ascorbic acid (A0278), ammonium iron (II) sulfate hexahydrate (215406), BSA (A3803), and β-nicotinamide adenine dinucleotide (NAD, N6522) were from Sigma-Aldrich. Tween-20 (10%, 28320) was from Thermo Fisher Scientific. 384 Alpha-plates, acceptor beads (5 mg/mL), alpha streptavidin donor beads (5 mg/mL, 6760002), and 5× Epigenetic buffer (AL008) were from PerkinElmer (Waltham, MA). Chicken core histones were obtained from Millipore (Billerica, MA). HeLa cell nucleosomes were from Reaction Biology Corporation (Malvern, PA). Nucleosome antigen was from Arotec Diagnosis (ATN02, Wellington, New Zealand). Small molecule inhibitors used as positive controls included S2101 (CalBiochem/Millipore; 489477), 2,4-Pyridinedicarboxylic acid (AK Scientific, Union City, CA; 00473), 8-hydroxyquinoline (Sigma-Aldrich; S018), 8-hydroxy-5-quinolinecarboxylic acid (Sigma-Aldrich; SML0057), S-(5′-Adenosyl)-L-homocysteine (Sigma-Aldrich; A9384), Trichostatin A (Sigma-Aldrich; T8552), and EX-527 (Sigma-Aldrich; E7024).