Skyscan 1276 micro ct

Rat knee joints were scanned using a SkyScan 1276 Micro-CT (Bruker, Kontich, Belgium) and NRecon version 1.6 software (Bruker). Data were then analyzed using the CTAn version 1.9 software (Bruker), and a three-dimensional model was generated in CTVol version 2.0 (Bruker). Quantitative morphometry indexes based on three-dimensional morphometry were determined using microtomographic data. Bone volume (BV), BV/total tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) were determined for the region of interest between the proximal tibia growth plate and tibial plateau.

Knee joint images were captured by X-ray (MX-20, Faxitron X-ray, Corp., Lincolnshire, IL, United States) and SkyScan 1276 Micro-CT (Bruker, Kontich, Belgium) and NRecon version 1.6 software (Bruker) and Ingenia3.0 T MRI system (Philips, United States). The rats were anesthetized by intraperitoneal injection of pentobarbital sodium (40 mg/kg) and fixed in supine position. The bilateral ankles were fixed on the tray with adhesive tape. The lens was focused at an appropriate focal length on the knee joint of the rat and the exposure time was set to apropriate minutes to ensure a clear image. The extent of osteoarthritis was assessed by imaging findings, including joint space narrowing and articular surface calcification, as well as articular cartilage damage, according to the imaging scoring system used in previous literature with macroscopic score which was based on surface roughness and erosin. Using imaging techniques, we quantified the tibial plateau or femoral condyle surface by calculating the ratio of the lesion area to the total surface area. Both the tibial and femoral joints were evaluated based on a maximum score of 10 (Gerwin et al., 2010 (link); Kohn et al., 2016 (link); Lin et al., 2021 (link)). The scoring was performed by two experienced observers who were blinded to the study groups.

At 12 and 24 h after modeling, six rats in each group were sedated with chloral hydrate, and their lungs were scanned with a SkyScan 1276 Micro-CT (Bruker, Billerica, MA, US) at 50-kV voltage, 70-μA current, and 40-μm voxel size. Analysis was performed using CTAn Software v1.17.9.0 (Blue Scientific Ltd., Cambridge, UK) and CTvox Software v3.3.0.0 (Blue Scientific Ltd., Cambridge, UK).

At the checkpoints after implantation, multispiral computed tomography (MSCT) and micro-CT were performed using a Toshiba Aquilion Prime 80 tomograph (Toshiba, Japan) and Bruker SkyScan 1276 micro-CT (Bruker, Belgium), respectively. In the first case, the obtained data were reconstructed using the VITREA software package (Vital Images, USA) and exported as a series of DICOM files, which were analyzed using the ORS Dragonfly software. In the second case, the procedures were described above.

All micro-CT imaging was performed using micro-CT system Bruker SkyScan 1276 Micro-CT. Animals were scanned while breathing freely under anesthesia using standard ketamine−xylazine anesthesia. Micro CT according to [10 (link)] was used to qualitative and semiquantitative image evaluation of the lung abnormalities by using a CT severity score adapted from a human scoring system.

Micro‐computed tomography (microCT) imaging was performed using a Bruker Skyscan 1276 microCT (Bruker), as previously described.²² Immediately following fat grafting, mice were imaged to determine baseline fat graft volume. Thereafter, serial imaging was performed every 2 weeks for a total of 8 weeks postgrafting. Three‐dimensional reconstructions were performed using cubic‐spline interpolation to determine fat graft volume as a percentage of the original transplanted volume.⁵ All reconstructions were performed blinded by two investigators (M. R. B. and S. V.) and the mean of each score was calculated.