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Colorimetric assay kit

Manufactured by Beyotime
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The Colorimetric assay kit is a laboratory equipment designed to measure the concentration of a specific substance in a sample through color-based analysis. The kit provides the necessary reagents and materials to perform quantitative colorimetric tests, which can be used to determine the presence and amount of various analytes, such as proteins, enzymes, or metabolites, in a variety of sample types.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Microplate reader, by Bio-Rad (2 mentions) Flx800, by Agilent Technologies (2 mentions) Elisa reader, by Agilent Technologies (1 mentions) Multiskan skyhigh, by Thermo Fisher Scientific (1 mentions) Enzymatic assay kit, by Beyotime (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions) Microplate reader, by Eppendorf (1 mentions) Bicinchoninic acid protein assay reagent, by Beyotime (1 mentions) Bca protein detection reagent, by Beyotime (1 mentions) Synergy h1 hybrid microplate reader, by Agilent Technologies (1 mentions) Bradford assay kit, by Beyotime (1 mentions) Pal 1, by Atago (1 mentions) Phenylmethanesulphonyl fluoride, by Merck Group (1 mentions)

35 protocols using colorimetric assay kit

1

Myocardial Caspase-3 and LDH Activity Assays

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Myocardial caspase-3 activity and serum LDH activity were determined by colorimetric assay kits (Beyotime Institute of Biotechnology, Jiangsu, China; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) as described in our previous study11 (link). The heart tissue and blood samples were collected from mice 3 days after MI and the activity of Caspase-3 and LDH were measured with the colorimetric method according to the manufacture’s protocols, respectively.
Sun C., Liu H., Guo J., Yu Y., Yang D., He F, & Du Z. (2017). MicroRNA-98 negatively regulates myocardial infarction-induced apoptosis by down-regulating Fas and caspase-3. Scientific Reports, 7, 7460.
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2

Caspase Activity Measurement in RK13 Cells

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Caspase-3 -8, and -9 activities were measured using colorimetric assay kits (Beyotime) according to manufacturer’s instructions. RK13 cells transfected with pcDNA3.1-NSP6 and pcDNA3.1-6 His vector for 24 h were harvested by centrifugation and incubated in lysis buffer on ice for 5 min. The lysate was then centrifuged at 15,000 rpm and 4 C for 15 min and the final protein content was determined using a Pierce BCA protein assay kit (Thermo Fisher Scientific). Next, 20 μg of protein was incubated for 2 h at 37 C with DEVD-pNA colorimetric substrate, IETD-pNA, and LEHD-pNA for the caspase-3, 8, and 9 assays, respectively. Activity was estimated by measuring absorption at 405 nm and subtracting the background values obtained from wells without colorimetric substrate.
Chen M., Liu X., Hu B., Fan Z., Song Y., Wei H., Qiu R., Xu W., Zhu W, & Wang F. (2019). Rabbit Hemorrhagic Disease Virus Non-structural Protein 6 Induces Apoptosis in Rabbit Kidney Cells. Frontiers in Microbiology, 9, 3308.
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3

Caspase Activity Induced by Esculetin

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Caspase-3, -9, and -8 activity induced by esculetin was determined by colorimetric
assay kits (Beyotime Institute of Biotechnology) according to the manufacturer's
instructions. All measurements were performed 3 times, and caspase activity was
determined by measuring changes in absorbance at 405 nm using an ELISA reader (BioTek
Instruments, Inc.).
Wang J., Lu M.L., Dai H.L., Zhang S.P., Wang H.X, & Wei N. (2014). Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway. Brazilian Journal of Medical and Biological Research, 48(3), 245-253.
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4

Hepatic Lipid Extraction and Assays

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Hepatic lipid was extracted using a modified Folch method [11 (link)]. Triglycerides were measured using commercially available colorimetric kit (BSBE, Beijing, China). Reduced glutathione (GSH) content was measured using the corresponding commercial colorimetric assay kits (Beyotime, Shanghai, China) according to the manufacturer's instructions.
He L., Liu Y., Liu D., Feng Y., Yin J, & Zhou X. (2021). Exogenous and Endogenous Serine Deficiency Exacerbates Hepatic Lipid Accumulation. Oxidative Medicine and Cellular Longevity, 2021, 4232704.
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5

Myocardial Caspase-3 Activity Assay

Cited 1 time
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Myocardial caspase-3 activity was determined by colorimetric assay kits (Beyotime Institute of Biotechnology), as described previously (18 (link)). The heart tissue was collected from mice 3 days after MI and the assays were performed according to the manufacturer's protocol.
Han H., Dai D., Hu J., Zhu J., Lu L., Tao G, & Zhang R. (2019). Dexmedetomidine improves cardiac function and protects against maladaptive remodeling following myocardial infarction. Molecular Medicine Reports, 20(6), 5183-5189.
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6

Colorimetric Assay for Caspase-1 Activity

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The Caspase-1 activities in all groups were performed by using colorimetric assay kits (Beyotime, Shanghai China) according to the manufacturer’s instruction. The assay kit is based on that acetyl-Tyr-Val-Asp p-nitroanilide (Ac-YVAD-pNA) can be catalyzed by caspase-1 to form p-nitroanilide (pNA), which has a strong absorption at 405 nm. The hepatocytes were collected and incubated in lysis buffer on ice for 30 min. And supernatant was collected after centrifugation, and protein concentration was measured by using “Modified Bradford Protein Assay Kit” (Sagon Biotech, Shanghai, China). Sample was incubated with substrate Ac-YVAD. The activity of caspase-1 was assessed by measuring the absorbance using a standard pNA curve.
Sun W., Zeng C., Liu S., Fu J., Hu L., Shi Z., Yue D., Ren Z., Zhong Z., Zuo Z., Cao S., Peng G., Deng J, & Hu Y. (2018). Ageratina adenophora induces mice hepatotoxicity via ROS-NLRP3-mediated pyroptosis. Scientific Reports, 8, 16032.
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7

Caspase-3 Activity Quantification in GCs

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As a marker of apoptosis, caspase-3 activity in GCs was determined by colorimetric assay kits (Beyotime Institute of Biotechnology, China) according to the manufacturer's instructions. In brief, GCs were lysed, and the assay was performed by incubating 10 μg of total protein in 100 μL of reaction buffer containing caspase-3 substrate and incubated for 2 h at 37°C. Caspase-3 activity was detected by a microplate reader (Bio-Rad, Hercules, CA, USA) at 405 nm.
Zhang J.Q., Wang X.W., Chen J.F., Ren Q.L., Wang J., Gao B.W., Shi Z.H., Zhang Z.J., Bai X.X, & Xing B.S. (2019). Grape Seed Procyanidin B2 Protects Porcine Ovarian Granulosa Cells against Oxidative Stress-Induced Apoptosis by Upregulating let-7a Expression. Oxidative Medicine and Cellular Longevity, 2019, 1076512.
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8

Oxidative Stress Markers in Infarct Border

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Total concentration of ROS (Cat. No. S0033S), total glutathione (GSH; Cat. No. S0052), the activities of total superoxide dismutase (SOD; Cat. No. S0109), and glutathione peroxidase (GPx; Cat. No. S0056) in infarct border zone tissues were examined using the corresponding colorimetric assay kits (Beyotime Biotechnology Co., Ltd., Shanghai, China) in accordance with the manufacturer's protocols.
Shen J.F., Fan Z.B., Wu C.W., Qi G.X., Cao Q.Y, & Xu F. (2022). Sacubitril Valsartan Enhances Cardiac Function and Alleviates Myocardial Infarction in Rats through a SUV39H1/SPP1 Axis. Oxidative Medicine and Cellular Longevity, 2022, 5009289.
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9

Colorimetric Caspase Activity Assay

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The activities of caspase-3, -8 and -9 in liver samples were determined using the colorimetric assay kits according to the manufacturer's instructions (Beyotime). Briefly, the homogenates of the liver samples were prepared and centrifuged for 1 min at 10 000 × g. The supernatant was incubated with Ac-DEVD-pNA, Ac-IETD-pNA and Ac-LEHD-pNA substrates for determination of caspase-3, -8 and -9, respectively, for 90 min at 37 °C. The activities of caspases were calculated according to the absorbance measured at 405 nm and the value was normalized by the protein concentration of the same sample.
Hu K., Gong X., Ai Q., Lin L., Dai J., Cai L., Jiang R., Ge P, & Zhang L. (2017). Endogenous AMPK acts as a detrimental factor in fulminant hepatitis via potentiating JNK-dependent hepatocyte apoptosis. Cell Death & Disease, 8(3), e2637-.
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10

Antioxidant Capacity and SOD Analysis

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The total antioxidant capacity (ABTS and FRAP) and SOD activity of samples were analyzed with colorimetric assay kits (Shanghai beyotime Biotechnology Co., Ltd., China, and Nanjing Jiancheng Bioengineering Institute, China).
Wang M.T., He Q.J., Guo J.K., Liu S.T., Ni L., Rao P.F., Chen T.B., Wu S.B., Zhao S.J., Qiao J.H., Zhang P.W, & Li Y.B. (2021). The Meridian Tropism and Classification of Red Yeast Rice Investigated by Monitoring Dermal Electrical Potential. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 1696575.
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