HEK293T (ATCC CRL-3216) and U2OS (ATCC-HTB-96) were maintained in Dulbecco's Modified Eagle's Medium plus GlutaMax (ThermoFisher) supplemented with 10% (v/v) fetal bovine serum (FBS), at 37 °C with 5% CO2. HCC1954 cells (ATCC CRL-2338) were maintained in RPMI-1640 medium (ThermoFisher Scientific) supplemented as described above. Immortalized rat astrocytes containing the ApoE4 isoform of the APOE gene (Taconic Biosciences) were cultured in Dulbecco's Modified Eagle's Medium plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v) fetal bovine serum (FBS) and 200 μg/mL Geneticin (ThermoFisher Scientific).
Apoe4 isoform of the apoe gene
Manufactured by Taconic Biosciences
The ApoE4 isoform of the APOE gene is a genetic variant that is associated with an increased risk of developing late-onset Alzheimer's disease. It is one of the three main isoforms of the APOE gene, which plays a role in the transport and metabolism of cholesterol in the body. The ApoE4 isoform differs from the more common ApoE3 isoform by a single amino acid substitution, which can impact its function.
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2 protocols using apoe4 isoform of the apoe gene
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Cell Culture Protocols for Cell Lines
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Cell Culture Protocols for Cell Lines
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HEK293T (ATCC CRL-3216) and U2OS (ATCC-HTB-96) were maintained in Dulbecco's Modified Eagle's Medium plus GlutaMax (ThermoFisher) supplemented with 10% (v/v) fetal bovine serum (FBS), at 37 °C with 5% CO2. HCC1954 cells (ATCC CRL-2338) were maintained in RPMI-1640 medium (ThermoFisher Scientific) supplemented as described above. Immortalized rat astrocytes containing the ApoE4 isoform of the APOE gene (Taconic Biosciences) were cultured in Dulbecco's Modified Eagle's Medium plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v) fetal bovine serum (FBS) and 200 μg/mL Geneticin (ThermoFisher Scientific).
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