Cells were fixed and stained for mouse anti–βIII-tubulin (1:2000, Promega), mouse anti-Tau1 (1:200, Chemicon MAB342), rabbit anti-Map2 (1:1000, EMD Millipore AB5622), or mouse anti-BrdU, (EMD Millipore, MAB3222) as described (55 (link)). Mitosis assay was performed as described (56 (link)). Cells were imaged at 4× or 10× with ImageXpress, and neurite outgrowth was automatically quantified by MetaXpress software (Molecular Devices).
Mab3222
Manufactured by Merck Group
MAB3222 is a laboratory equipment product manufactured by Merck Group. It is a specialized instrument designed for research and analytical applications. The core function of this product is to facilitate specific tasks or procedures in the laboratory environment, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mab3222
1
Immunocytochemical Analysis of Neuronal Markers
2
BrdU Incorporation Assay for DNA Synthesis
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DNA synthesis of the cells was assessed by the BrdU incorporation assay (Roche Applied Science, Penzberg, Germany). After 48 hours of transfection of the indicated shRNAs, the cells of each group were reseeded in 96-well plates at a density of 5×103 cells/well. Cells were incubated with 10 µM BrdU for 4 hours and then were fixed with 4% paraformaldehyde. After being rinsed with PBS, cells were treated with 2N HCl for 30 minutes. Nonspecific binding was blocked in 5% BSA for 1 hour at room temperature. Cells were respectively incubated with mouse anti-BrdU (1:200, MAB3222; EMD Millipore) overnight at 4°C one after another. Then followed by three times washes in PBS, they were incubated with Goat Anti-Mouse IgG H&L (Cy5®) preadsorbed (ab6563) for 1 hour at room temperature. Immunofluorescence was observed using flow cytometry.
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