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Lsrfortessa

Manufactured by Thermo Fisher Scientific
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The LSRFortessa is a flow cytometry instrument designed for multi-parameter analysis. It is capable of detecting and analyzing multiple fluorescent signals simultaneously from cells or other particles in a liquid sample. The LSRFortessa utilizes advanced optics and detectors to provide high-sensitivity and high-resolution data.

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Lab products found in correlation

Anti cd16 cd32, by Thermo Fisher Scientific (3 mentions) Fixation permeabilization kit, by BD (3 mentions) Lsrfortessa, by BD (3 mentions) Golgistop, by BD (3 mentions) Lsrii facsfortessa, by BD (1 mentions)

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LSRFortessa flow cytometer (7 citations)

3 protocols using lsrfortessa

1

Multiparametric Flow Cytometry Analysis

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Single-cell suspensions of spleen, mesenteric lymph nodes, Peyer’s patches, lamina propria, bone marrow and thymus were subjected to flow cytometry using an LSRFortessa (Becton Dickinson). The cells were incubated with anti-CD16/CD32 (eBioscience) to block nonspecific antibody binding via Fc receptors and then stained with fluorochrome-labeled antibodies, and subsequently processed on an LSRFortessa. The data were analyzed using FlowJo software (Tree Star). Gating strategies are summarized in Supplementary Fig. 9. For ICS detection of IFN-γ, IL-17 and IL-22, cells were stimulated for 4–5 h with PMA (50 ng/ml) and ionomycin (750 ng/ml) in the presence of GolgiStop (BD Bioscience). After incubation, cells were stained with fixable viability dye, blocked with FcγR blocker (CD16/32), and stained for specific surface molecules. After surface staining, cells were fixed, permeabilized and stained for intracellular cytokines by using a fixation/permeabilization kit (BD Biosciences). Samples were processed on an LSRFortessa (Becton Dickinson) and analyzed by using FlowJo software (TreeStar).
Jie Z., Yang J.Y., Gu M., Wang H., Xie X., Li Y., Liu T., Zhu L., Shi J., Zhang L., Zhou X., Joo D., Brightbill H.D., Cong Y., Lin D., Cheng X, & Sun S.C. (2018). NIK signaling axis regulates dendritic cell function in intestinal immunity and homeostasis. Nature immunology, 19(11), 1224-1235.
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2

Multiparametric Flow Cytometry Analysis

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Single-cell suspensions of spleen, mesenteric lymph nodes, Peyer’s patches, lamina propria, bone marrow and thymus were subjected to flow cytometry using an LSRFortessa (Becton Dickinson). The cells were incubated with anti-CD16/CD32 (eBioscience) to block nonspecific antibody binding via Fc receptors and then stained with fluorochrome-labeled antibodies, and subsequently processed on an LSRFortessa. The data were analyzed using FlowJo software (Tree Star). Gating strategies are summarized in Supplementary Fig. 9. For ICS detection of IFN-γ, IL-17 and IL-22, cells were stimulated for 4–5 h with PMA (50 ng/ml) and ionomycin (750 ng/ml) in the presence of GolgiStop (BD Bioscience). After incubation, cells were stained with fixable viability dye, blocked with FcγR blocker (CD16/32), and stained for specific surface molecules. After surface staining, cells were fixed, permeabilized and stained for intracellular cytokines by using a fixation/permeabilization kit (BD Biosciences). Samples were processed on an LSRFortessa (Becton Dickinson) and analyzed by using FlowJo software (TreeStar).
Jie Z., Yang J.Y., Gu M., Wang H., Xie X., Li Y., Liu T., Zhu L., Shi J., Zhang L., Zhou X., Joo D., Brightbill H.D., Cong Y., Lin D., Cheng X, & Sun S.C. (2018). NIK signaling axis regulates dendritic cell function in intestinal immunity and homeostasis. Nature immunology, 19(11), 1224-1235.
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3

Flow Cytometry and Intracellular Cytokine Staining

Cited 1 time
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Flow cytometry and intracellular cytokine staining (ICS) were performed following procedures previously described (46 (link)). Briefly, single-cell suspensions were prepared from the indicated tissues and subjected to flow cytometry using an LSRFortessa (Becton Dickinson, San Jose, CA). The cells were incubated with anti-CD16/CD32 (eBioscience) to block nonspecific antibody binding via Fc receptors, then stained with fluorochrome-labeled antibodies, and subsequently processed on an LSRFortessa. The data were analyzed using FlowJo software (Tree Star, Ashland, OR), and gating strategy is shown in fig. S8. For ICS detection of IFNγ, IL-17, and GM-CSF, cells were stimulated for 4 to 5 hours with phorbol 12-myristate 13-acetate (50 ng/ml) and ionomycin (750 ng/ml) in the presence of GolgiStop (BD Biosciences). After incubation, cells were stained with fixable viability dye, blocked with FcγR blocker (CD16/32), and stained for specific surface molecules. After surface staining, cells were fixed, permeabilized, and stained for intracellular cytokines by using a fixation/permeabilization kit (BD Biosciences). Samples were processed on an LSRII FACSFortessa (Becton Dickinson, San Jose, CA) and analyzed by using FlowJo software (TreeStar, Ashland, OR).
Jie Z., Ko C.J., Wang H., Xie X., Li Y., Gu M., Zhu L., Yang J.Y., Gao T., Ru W., Tang S.J., Cheng X, & Sun S.C. (2021). Microglia promote autoimmune inflammation via the noncanonical NF-κB pathway. Science Advances, 7(36), eabh0609.
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