Lidocaine hcl

Manufactured by Pfizer

Sourced in United States

Lidocaine HCl is a local anesthetic agent. It is a hydrochloride salt of lidocaine, a widely used numbing medication. Lidocaine HCl is commonly used in medical and dental procedures to provide local or topical anesthesia.

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Lab products found in correlation

I fect, by Neuromics (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Epinephrine, by Pfizer (1 mentions)

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Lidocaine

5 protocols using lidocaine hcl

Muscle Biopsy and Tissue Processing

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The muscle biopsy was completed on the leg that was not used to collect ³¹P-MRS data. Participants provided approximately 600 mg of muscle using the Bergstrom technique^{20 (link)} in the vastus lateralis. Briefly, the skin surface area was disinfected with 7.5% povidone-iodine prep solution (Aplicare, Forest Hills, NY). A topical anesthesia was induced with a mixture of 2% lidocaine HCl (Hospira, Lake Forest, IL) and 0.5% bupivacaine HCl (AUROMEDICS Pharma, Dayton, NJ) to produce local anesthetic effects at the location of muscle biopsy. A 0.75 cm incision was made into the skeletal muscle fascia, fascia fibers were teased apart with the blunt edge of the scalpel, the Bergstrom needle was inserted to remove muscle tissue, and the wound was closed using bandage. The tissue was further processed for mRNA and protein extractions.

Darpolor M.M., Singh M., Covington J., Hanet S., Ravussin E, & Carmichael O.T. (2020). Molecular correlates of MRS-based 31phosphocreatine muscle resynthesis rate in healthy adults. NMR in biomedicine, 34(1), e4402.

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Radiofrequency Neuromodulation for Lumbar DRG

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The patient placed in the prone position with a pillow under the lower abdomen to provide an easy approach to the intervertebral foramen. Under complete aseptic condition, 2 ml of 1% lidocaine (Lidocaine HCl, Hospira, Inc., Lake Forest, USA) used for local anesthesia of the skin prior to the placement of the RF electrode (22-G, 10 cm needle, with a curved 10 mm active tip, Neurotherm) under C-arm fluoroscopic control. In the lumbar segments, the electrode was positioned near to the DRG, which typically corresponded to the dorsal-cranial quadrant of the intervertebral foramen on lateral view, and on anteroposterior view, the tip was located midway in the pedicle column. The electrode advancement was depending on the stimulation criteria below. Once the electrode was in a correct position, the stylet then replaced by the radiofrequency probe. PRF current applied for 20 ms, at 2 Hz, for 120 seconds. The maximum target temperature was 42°C (Neurotherm 1100).
The final positional confirmed by the following methods:
Weak muscle contraction above 2 volts was accepted to be targeted treatment. PRF current applied for 20 ms, at 2 Hz, for 120 second. The maximum target temperature was 42°C (Neurotherm 1100).

Abdelrahman K.A., Ibrahim A.S., Osman A.M., Aly M.G., Ali A.S, & Farrag W.S. (2021). Alpha lipoic acid with pulsed radiofrequency in treatment of chronic lumbosacral radicular pain: A prospective, randomized study. Medicine, 100(24), e26344.

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Intrathecal Delivery of CaMKIIα siRNA

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2-[N-(2-hydroxyethyl)]-N-(4-methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine) (KN93) and 2-[N-(4-Methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine (KN92) were purchased from Tocris Bioscience (Ellisville, MO). Lidocaine HCl (2%) was from Hospira (Lake Forest, IL). Other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). CaMKIIα siRNA (sense, 5′-CACCACCAUUGAGGACGAAdTdT-3′, antisense, 5′-UUCGUCCUCAAUGGUGdTdT-3′) and scrambled RNA duplex control (sense, 5′-AUACGCGUAUUAUACGCGAUUACGAC-3′; antisense, 5′-CGUUAAUCGCGUAUAAUACGCGUAT-3′) were synthesized by Integrated DNA Technologies (Coralville, IW). Lidocaine, KN93, KN92 and RNA duplexes were administered intrathecally (i.t.) in a volume of 5 μL by percutaneous puncture through the L5–L6 intervertebral space.^{26 (link),32 (link)} The RNAs were mixed with a transfection reagent i-Fect (Neuromics, Minneapolis, MN) at a ratio of 1:5 (w/v).^{7 (link)}

He Y., Chen Y., Tian X., Yang C., Lu J., Xiao C., DeSimone J., Wilkie D.J., Molokie R.E, & Wang Z.J. (2016). CaMKIIα underlies spontaneous and evoked pain behaviors in Berkeley sickle cell transgenic mice. Pain, 157(12), 2798-2806.

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Lidocaine Treatment Effects on hMSCs

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Human MSCs were seeded and incubated at 37 °C in humidified atmosphere with 5% carbon dioxide for 24 hours. Reagents used in this study include: 1% lidocaine HCl injection (10mg/ml, lot #: 52–400-DK, Hospira, Inc., Lake Forest, IL) and phosphate buffered saline (1× PBS pH 7.4, lot #: 1745964, Life technologies, NY). Lidocaine was diluted with different amounts of complete media to reach the desired concentrations. After 24 hours of culturing in vitro, MSCs were washed with advanced minimum essential medium (MEM) and incubated with 0.8% (8 mg/ml), 0.4% (4 mg/ml), 0.2% (2mg/ml) and 0.1% (1mg/ml) lidocaine for 0.5, 1, 2 and 4 hours. PBS solution served as a control. After incubation with lidocaine or PBS, the treatment fluids were aspirated, and single media was added into the culture plates. Cells viability, proliferation and function were analyzed immediately post-exposure (0 hour), as well as at 24 hours and 48 hours following treatment. Time course and concentration dependent experiments were performed on three sets of cell lines.

Nie H., Kubrova E., Wu T., Denbeigh J.M., Hunt C., Dietz A.B., Smith J., Qu W, & van Wijnen A.J. (2019). Effect of lidocaine on viability and gene expression of human adipose derived mesenchymal stem cells: an in vitro study. PM & R : the journal of injury, function, and rehabilitation, 11(11), 1218-1227.

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Stereotaxic Implantation of Microelectrodes in Murine Parietal Cortex

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Thirteen female C57BL/6J black mice aged 3 months weighing 20–24 g were used in this study. All procedures were conducted in accordance with a protocol approved by the UCSF Institutional Animal Care and Use Committee.
Mice were anesthetized with a mixture of 2.0 mg ketamine HCl and 0.3 mg xylazine HCl delivered intraperitoneally. They were placed in a stereotaxic frame and supported on a heating pad. The scalp was infiltrated with 1% lidocaine HCl with epinephrine 1:100,000 (Hospira, Inc., Lake Forest, Il). A 4 mm sagittal incision was made to expose the skull. 1 mm diameter burr holes were drilled 1.25 mm on either side of the sagittal suture, centered 1.75 mm anterior to the interaural line, to expose the dura over the parietal cortex. After perpendicular microelectrode penetrations were made, the scalp was closed with two 7–0 polyglactin sutures. Mice survived for varying time intervals to evaluate the persistence of various compounds used to label the electrode tracks (Table 1).

Simmons J.B., Turner R.S, & Horton J.C. (2020). Long-term Labeling of Microelectrode Tracks with Fluorescent Latex Microspheres. Journal of neuroscience methods, 343, 108839.

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