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1 hydroxymidazolam

Manufactured by Cayman Chemical
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1'-hydroxymidazolam is a chemical compound used in research and analytical applications. It serves as a metabolite and analytical reference standard. The core function of 1'-hydroxymidazolam is to provide a reliable and well-characterized substance for research, development, and quality control purposes.

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Lab products found in correlation

Dextrorphan, by Cayman Chemical (3 mentions) Midazolam, by Cayman Chemical (3 mentions) Mitragynine, by Cayman Chemical (3 mentions) 7 hydroxymitragynine, by Cayman Chemical (3 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Dextromethorphan, by Cayman Chemical (2 mentions) Dextromethorphan d3, by Cayman Chemical (2 mentions) 4 hydroxymidazolam, by Cayman Chemical (2 mentions) Midazolam d4, by Cayman Chemical (2 mentions) Mitragynine d3, by Cayman Chemical (2 mentions) Tert butyl methyl ether, by Merck Group (2 mentions) Dextromethorphan, by LGC (2 mentions) Dextrorphan, by LGC (2 mentions) Dextromethorphan, by Merck Group (2 mentions) 4 hydroxy mephenytoin, by Cayman Chemical (2 mentions) Diclofenac, by Merck Group (2 mentions) Hplc grade acetonitrile, by Merck Group (2 mentions) Phenacetin, by Merck Group (2 mentions) Sulfaphenazole, by Cayman Chemical (1 mentions)

10 protocols using 1 hydroxymidazolam

1

Quantification of Kratom Alkaloids in Plasma

Cited 2 times
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Dextromethorphan, Dextromethorphan-d3, dextrorphan, 1′-hydroxymidazolam, 4-hydroxymidazolam, 7-hydroxymitragynine, midazolam, midazolam-d4, mitragynine, and mitragynine-d3 were purchased from Cayman Chemical (Ann Arbor, MI). The kratom indole alkaloids speciociliatine, speciogynine, mitraciliatine, paynantheine, and isopaynantheine were isolated and purified from commercial kratom products as described3 (link),5 (link); all reference standards were verified to be ≥95% pure as measured by ultra-high-performance liquid chromatography-ultraviolet spectroscopy (UHPLC-UV) analysis. tert-Butyl methyl ether was purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Blank human plasma (K2EDTA, pooled, mixed gender, lot HMN99236) was purchased from BioIVT (Westbury, NY). All other chemicals and reagents were analytical grade.
Naiman B.M., Alt D., Bolin C.A., Zuerner R, & Baldwin C.L. (2001). Protective Killed Leptospira borgpetersenii Vaccine Induces Potent Th1 Immunity Comprising Responses by CD4 and γδ T Lymphocytes. Infection and Immunity, 69(12), 7550-7558.
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2

Quantification of Kratom Alkaloids in Plasma

Cited 2 times
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Dextromethorphan, Dextromethorphan-d3, dextrorphan, 1′-hydroxymidazolam, 4-hydroxymidazolam, 7-hydroxymitragynine, midazolam, midazolam-d4, mitragynine, and mitragynine-d3 were purchased from Cayman Chemical (Ann Arbor, MI). The kratom indole alkaloids speciociliatine, speciogynine, mitraciliatine, paynantheine, and isopaynantheine were isolated and purified from commercial kratom products as described3 (link),5 (link); all reference standards were verified to be ≥95% pure as measured by ultra-high-performance liquid chromatography-ultraviolet spectroscopy (UHPLC-UV) analysis. tert-Butyl methyl ether was purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Blank human plasma (K2EDTA, pooled, mixed gender, lot HMN99236) was purchased from BioIVT (Westbury, NY). All other chemicals and reagents were analytical grade.
Tanna R.S., Nguyen J.T., Hadi D.L., Layton M.E., White J.R., Cech N.B., Oberlies N.H., Rettie A.E., Thummel K.E, & Paine M.F. (2023). Clinical Assessment of the Drug Interaction Potential of the Psychotropic Natural Product Kratom. Clinical pharmacology and therapeutics, 113(6), 1315-1325.
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3

Cytochrome P450 Enzyme Inhibition Assay

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DAMGO, quinidine, ketoconazole, morphine sulfate, and alprazolam were purchased from MilliporeSigma (St Louis, MO). Mitragynine, 7-hydroxyMitragynine, midazolam, 1′-hydroxymidazolam, sulfaphenazole, and NADPH were obtained from Cayman Chemical Company (Ann Arbor, MI). Diclofenac, 4′-hydroxyDiclofenac, dextromethorphan, and dextrorphan were obtained from Toronto Research Chemicals, Inc. (Toronto, Canada). Potassium phosphate buffer salts were obtained from Fisher Scientific (Fair Lawn, NJ). Human liver microsomes were obtained from XenoTech, LLC (H0604, mixed gender, pool from 15 donors, lot no. 1010191; Kansas City, KS). All other chemicals and reagents used were analytical grade.
Todd D.A., Kellogg J.J., Wallace E.D., Khin M., Flores-Bocanegra L., Tanna R.S., McIntosh S., Raja H.A., Graf T.N., Hemby S.E., Paine M.F., Oberlies N.H, & Cech N.B. (2020). Chemical composition and biological effects of kratom (Mitragyna speciosa): In vitro studies with implications for efficacy and drug interactions. Scientific Reports, 10, 19158.
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4

Quantitative analysis of metabolites

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Garcinol (CAS #78824-30-3), 1-hydroxy Tacrine, hydroxybupropion 4-hydroxy diclofenac, 6-hydroxy paclitaxel, 4-hydroxy mephenytoin, 1-hydroxy midazolam, 6-hydroxychlorzoxazone, and dextrorphan were procured from Cayman Chemical Company (Ann Arbor, MI, USA). Rat liver microsomes were procured from BioreclamationIVT (Baltimore, MD, USA). Tacrine, bupropion, S-mephenytoin, diclofenac, paclitaxel, dextromethorphan, chlorzoxazone, and midazolam, dimethylsulfoxide (DMSO), phosphate buffered saline (PBS), and nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt hydrate (NADPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Formic acid and acetonitrile (HPLC grade) (>98% purified) were procured from Merck Specialities Pvt. Ltd. (Mumbai, India). Millipore water (in-house) was used for the entire study.
Bolla L., Srivastava P., Ravichandiran V, & Nanjappan S.K. (2021). Cytochrome P450 and P-gp Mediated Herb-Drug Interactions and Molecular Docking Studies of Garcinol. Membranes, 11(12), 992.
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5

Microfluidic Tissue Chip Protocol

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Phenacetin (77440, CAS#62-44-2), coumarin (C4261, CAS#91-64-5), diclofenac (D6899, CAS#15307-79-6), terfenadine (T9652, CAS#50679-08-8), phenolphthalein (105945, CAS#77-09-8), acetaminophen (PHR1005, CAS#103-90-2) and 4-hydroxy diclofenac (32412, CAS#64118-84-9), fexofenadine (PHR1685, CAS#153439-40-8), lipopolysaccharide (LPS; L2630), troglitazone (T2573, CAS#97322-87-7), and trovafloxacin (TVX; PZ0015, CAS#147059-75-4) were purchased from Millipore Sigma (Burlington, MA). Midazolam (19391, CAS#59467-70-8), 1’-hydroxymidazolam (10385, CAS#59468-90-5), and phenolphthalein β-D-glucuronide (30424, CAS#15265-26-6) were purchased from Cayman Chemical (Ann Arbor, MI).
The microfluidic tissue chips used in this study were purchased from Mimetas (OrganoPlate® 2-lane 96, Leiden, Netherlands). Each chip of this 96-well platform contains one gel channel and one perfusion channel. This configuration enables the culture of a perfused tubule adjacent to extracellular matrix (ECM) of choice without a membrane (Bircsak et al., 2021 (link)). Black-walled, clear-bottom, tissue culture-treated 96-well (3603, Corning, Corning, NY) and 384-well plates (3765, Corning) were used for 2D cell culture experiments.
Grote E., Vlacich G., Pypaert M, & Novick P.J. (2000). A snc1 Endocytosis Mutant: Phenotypic Analysis and Suppression by Overproduction of Dihydrosphingosine Phosphate Lyase. Molecular Biology of the Cell, 11(12), 4051-4065.
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6

Chemical Characterization of Medicinal Compounds

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All compounds were obtained from commercial sources and the purity was
95% or higher. The compound name, vendor, catalog number, and analytical method
used to determine purity are: Ritonavir, Toronto Research Chemicals, R535000,
1H NMR; 1-hydroxymidazolam, Cayman Chemical, 10385, 1H
NMR; Clobetasol propionate, AK Scientific, F535, HPLC; Ketoconazole, Abovchem,
AC513267, 1H NMR; Midazolam, U.S. Pharmacopeia, 1443599, UHPLC and
LC-MS/MS.
Wright W.C., Chenge J., Wang J., Girvan H.M., Yang L., Chai S.C., Huber A.D., Wu J., Oladimeji P.O., Munro A.W, & Chen T. (2020). Clobetasol propionate is a heme-mediated selective inhibitor of human cytochrome P450 3A5. Journal of medicinal chemistry, 63(3), 1415-1433.
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7

Cytochrome P450 Enzyme Regulation

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Repaglinide and midazolam from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Atorvastatin calcium and gemfibrozil from Dalian Meilun Biology Technology Co, Ltd (Dalian, China). Ketoconazole and rifampicin from J&K Chemical (Shanghai, China). 1-hydroxymidazolam from Cayman Chemical (Ann Arbor, MI, USA). Phenobarbital from New Asia Pharmaceutical Company (Shanghai, China). Streptozotocin (STZ) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) from Sigma Chemical Co (St Louis, MO, USA). Para-hydroxy atorvastatin calcium and ortho-hydroxy atorvastatin calcium from TLC PharmaChem Inc. (Canada). Glutathione (GSH) and N-acetylcystiene from Sunshine Biotechnology Co. Ltd (Nanjing, China). Mouse monoclonal Cyp3a, CYP3A4 and SLCO1B1 antibodies from Santa Cruz Technology (Santa Cruz, CA, USA). GAPDH antibody from Bioworld (Louis Park, MN, USA). Silencer® Pre-Designed siRNA (CYP3A4), Silencer® Pre-Designed siRNA (SLCO1B1), Silencer® Negative Control siRNA, Lipofectamine1 RNAiMAX Transfection Reagent and Opti-MEM® I Reduced Serum Medium were purchased from Molecular Probes (Invitrogen, Burlington, Ontario, Canada). All the other reagents were commercially available.
Shu N., Hu M., Ling Z., Liu P., Wang F., Xu P., Zhong Z., Sun B., Zhang M., Li F., Xie Q., Liu X, & Liu L. (2016). The enhanced atorvastatin hepatotoxicity in diabetic rats was partly attributed to the upregulated hepatic Cyp3a and SLCO1B1. Scientific Reports, 6, 33072.
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8

Quantitative Bioanalysis of Pharmaceuticals

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Caffeine, omeprazole, and propranolol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dextromethorphan, dextrorphan, midazolam, 5′-hydroxyomeprazole, losartan, losartan carboxylic acid (EXP3174), paraxanthine, and pitavastatin were obtained from Toronto Research Chemicals Inc. (North York, ON, Canada). 1′-Hydroxymidazolam was purchased from Cayman Chemical (Ann Arbor, MI, USA). Solvents were LC-MS grade (Fisher Scientific Co., Pittsburgh, PA, USA) and the other chemicals were obtained from Sigma-Aldrich. Pooled human plasma was obtained from BioChemed Services (Winchester, VA, USA).
Heo J.K., Kim H.J., Lee G.H., Ohk B., Lee S., Song K.S., Song I.S., Liu K.H, & Yoon Y.R. (2018). Simultaneous Determination of Five Cytochrome P450 Probe Substrates and Their Metabolites and Organic Anion Transporting Polypeptide Probe Substrate in Human Plasma Using Liquid Chromatography-Tandem Mass Spectrometry. Pharmaceutics, 10(3), 79.
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9

In Vitro Iohexol Elimination Assay

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Sterile sodium chloride (0.9% NaCl) and phosphate-buffered saline (PBS) were purchased from Baxter Healthcare Corporation (Deerfield, IL). Iohexol powder, paclitaxel, and gemcitabine were purchased from Toronto Research Chemicals Inc (Toronto, ON, Canada). Iohexol dosing solution was manufactured by GE Healthcare, (Shanghai, China) and obtained from the Hillman Cancer Research Pharmacy. All in vitro drug elimination incubations with iohexol were performed at 300 μg/mL which is at the upper range of the reported plasma concentrations [23 (link)]. [D5]-iohexol (IS) was purchased from ALSACHIM (Graffenstaden, France). Carboplatin for dosing was manufactured by Ben Venue Labs Inc (Bedford, OH) and obtained from the Hillman Cancer Research Pharmacy. NADPH tetrasodium salt was purchased from Enzo Life Sciences (Farmingdale, NY). Carboplatin powder, MgCl2, alamethicin, UDPGA sodium salt, phenacetin, dextromethorphan and midazolam methanolic solution was obtained from Sigma (St Louis, MO). [D3]-dextrorphan and [D4]-1-hydroxy midazolam were purchased from Sigma (St. Louis MO). 1-hydroxy midazolam, 4-hydroxy diclofenac, 4-hydroxy mephenytoin, dextrorphan tartrate, s-mephenytoin, SN-38, trifluoperazine, mycophenolic acid and naloxone were purchased from Cayman chemicals (Ann Arbor, MI).
Joshi A., Guo J., Holleran J.L., Kiesel B., Taylor S., Christner S., Parise R.A., Miller B.M., Ivy S.P., Chu E., Venkataramanan R, & Beumer J.H. (2020). EVALUATION OF THE PHARMACOKINETIC DRUG-DRUG INTERACTION POTENTIAL OF IOHEXOL, A RENAL FILTRATION MARKER. Cancer chemotherapy and pharmacology, 86(4), 535-545.
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10

Quantitative LC-MS/MS Bioanalysis

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Phenacetin (99.9%, T0778), acetaminophen (99.9%, T0065), tolbutamide (99.7%, T1054), metoprolol (99.8%, T0487), and chlorzoxazone (99.7%, T1650) were purchased from Target Molecule Corp. (Massachusetts, USA); omeprazole (> 99%, MB1692-S) from Dalian Meilun Biotech Co., Ltd. (Dalian, China); midazolam (5 mg/mL, H10980025) from Jiangsu Enhua Pharmaceutical Co., Ltd. (Xuzhou, China); 4 -hydroxytolbutamide (100%, H969850), 5 -hydroxyomepreole (95%, H948110), α -hydroxymetoprolol (100%, H948390), and 6 -hydroxychlorzoxazone (100%, H825120) from Toronto Research Chemicals (Toronto, Canada); 1hydroxymidazolam (95%, 10385-1) from Cayman Chemicals (Michigan, USA); and lamotrigine (100775-200401) from National Institutes for Food and Drug Control (Beijing, China). Glycyrrhetic acid (> 98%, B20412) was obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China) and used as internal standard (IS). HPLC-grade acetonitrile and methanol were purchased from Merck Company (Darmstadt, Germany); formic acid from CNW Technologies (Dusseldorf, Germany); phosphate buffer saline from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); ReadiUse NADPH Regenerating Kit from Wuhan PrimeTox Bio-pharma Technology Co., Ltd. (Wuhan, China); and deionized water from a Millipore Milli-Q Plus system (Bedford, USA).
Guo J., Xu Y., Chen L., Zhang S., Rao T., Peng J., Guo Y., Tan Z., Ouyang D., Chen X., Liou Y., Zhang W., & Chen Y. (2021). Gut Microbiota and Host Cyp450s Co-contribute to Pharmacokinetic Variability in Mice With Non-Alcoholic Steatohepatitis: Vary From Drug to Drug.
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