Dulbecco's modified eagle's medium (DMEM), fetal bovine serum (FBS), and lyophilized trypsin-EDTA were obtained from GIBCO BRL (Grand Island, NY, USA). 3-[4,5-Dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), and Tris/HCl were purchased from Sigma (St. Louis, MO, USA). The whole protein extraction kits were purchased from Keygen (Keygen Biotech. Co. Ltd., Nanjing, China). Trizol reagent, PrimeScript RT Master Mix, and SYBR Green PCR Master Mix reagent kits were obtained from TaKaRa (TaKaRa Biotechnology, Dalian, China). The primers were synthesized by Invitrogen Life Tech (Carlsbad, CA, USA). Rabbit monoclonal anti-AKT (phospho S473), rabbit polyclonal anti-Plzf ab38739, and anti-Oct-4 ab18976 were from Abcam (Cambridge, MA, USA). Mouse monoclonal anti-β-actin was from Chemicon (Temecula, CA). Enhanced chemiluminescence was obtained from Amersham Biosciences (Uppsala, Sweden).
3 4 5 dimethylthiazolyl 2 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso sodium dodecyl sulfate
Manufactured by Merck Group
Sourced in United States
3-[4,5-Dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide (MTT) is a tetrazolium salt. Dimethyl sulfoxide (DMSO) is an organic compound. Sodium dodecyl sulfate (SDS) is an anionic surfactant. These are commonly used as laboratory reagents.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence, by Cytiva (2 mentions)
Anti β actin, by Merck Group (2 mentions)
Tris hcl, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Lyophilized trypsin edta, by Thermo Fisher Scientific (2 mentions)
Sybr green pcr master mix reagent kit, by Takara Bio (2 mentions)
Anti akt phospho s473, by Abcam (2 mentions)
Rabbit polyclonal anti plzf ab38739, by Abcam (2 mentions)
Anti oct4 ab18976, by Abcam (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Primescript rt master mix, by Takara Bio (2 mentions)
Trizol reagent, by Takara Bio (2 mentions)
Whole protein extraction kit, by Keygen Biotech (2 mentions)
2 protocols using 3 4 5 dimethylthiazolyl 2 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso sodium dodecyl sulfate
1
Cytotoxicity and Gene Expression Analysis
2
Cytotoxicity and Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco's modified eagle's medium (DMEM), fetal bovine serum (FBS), and lyophilized trypsin-EDTA were obtained from GIBCO BRL (Grand Island, NY, USA). 3-[4,5-Dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), and Tris/HCl were purchased from Sigma (St. Louis, MO, USA). The whole protein extraction kits were purchased from Keygen (Keygen Biotech. Co. Ltd., Nanjing, China). Trizol reagent, PrimeScript RT Master Mix, and SYBR Green PCR Master Mix reagent kits were obtained from TaKaRa (TaKaRa Biotechnology, Dalian, China). The primers were synthesized by Invitrogen Life Tech (Carlsbad, CA, USA). Rabbit monoclonal anti-AKT (phospho S473), rabbit polyclonal anti-Plzf ab38739, and anti-Oct-4 ab18976 were from Abcam (Cambridge, MA, USA). Mouse monoclonal anti-β-actin was from Chemicon (Temecula, CA). Enhanced chemiluminescence was obtained from Amersham Biosciences (Uppsala, Sweden).
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