Dako autostainer plus en vision kit

Manufactured by Agilent Technologies

Sourced in Denmark

The Dako Autostainer Plus En Vision+ Kit is a laboratory equipment used for automated immunohistochemistry (IHC) and in situ hybridization (ISH) staining of tissue sections. It provides a standardized and consistent staining process by automating the steps involved in IHC and ISH protocols.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd45, by Abcam (1 mentions) Anti pstat3 t705, by Abcam (1 mentions) Anti pstat3 s727, by Cell Signaling Technology (1 mentions) Anti foxp3, by Abcam (1 mentions) Anti pstat3 tyr705, by Abcam (1 mentions)

Close spelling variants of this product

Dako Autostainer/Autostainer Plus (3 citations) Dako Autostainer Plus (69 citations) Dako Autostainer (170 citations) Autostainer Plus (172 citations) Dako kits (2 citations) Autostainer (227 citations)

2 protocols using dako autostainer plus en vision kit

Immunohistochemical and Immunofluorescent Analysis of RM-1 Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin fixed and paraffin embedded RM-1 mouse tumors were subjected to immunohistochemistry (IHC) using Dako Autostainer Plus En VisionTM + Kit (Dako, Glostrup, Denmark) and stained with the antibodies anti-pSTAT3-T705 (ab76315 Abcam, Cambridge, UK), anti-pSTAT3-S727 (#9143 Cell Signaling Technology, Danvers, MA, USA), anti-FOXP3 (ab54501 Abcam) and anti-CD45 (ab25386 Abcam). The immunostainings were analyzed using Halo image analysis software (Indica Laboratories, Albuquerque, NM, USA).
For immunofluorescent staining, the RM-1 tumors were stained using the Opal Multiplex immunofluorescence assay (Akoya Biosciences) and imaged with Mantra multispectral image system (Akoya Biosciences). Antibodies used were anti-CD3E (LS-C343957 LSBio), anti-CTLA-4 (ab 237,712 Abcam) and anti-pSTAT3-S727 (#9143 Cell Signaling Technology).

Witt K., Evans-Axelsson S., Lundqvist A., Johansson M., Bjartell A, & Hellsten R. (2021). Inhibition of STAT3 augments antitumor efficacy of anti-CTLA-4 treatment against prostate cancer. Cancer Immunology, Immunotherapy, 70(11), 3155-3166.

+ Open protocol

+ Expand

Immunostaining of STAT3 Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Suspensions of monocytes cultured for 72 hours in CM from prostate cancer cells or MDSCs isolated from mCRPC patients were placed in a cytospin funnel clamped to a glass slide and spun at 800 rpm for 2 minutes. The slides were air‐dried, fixed in 4% formaldehyde for 10 minutes, and permeabilized with 1% Triton‐X Tris buffer, pH 7.6 for 1 hour. The slides were subjected to immunohistochemistry using Dako Autostainer Plus En Vision^TM+ Kit (Dako). Cells were stained using the antibody anti‐pSTAT3 Tyr‐705 (ab76315; Abcam).

Hellsten R., Lilljebjörn L., Johansson M., Leandersson K, & Bjartell A. (2019). The STAT3 inhibitor galiellalactone inhibits the generation of MDSC‐like monocytes by prostate cancer cells and decreases immunosuppressive and tumorigenic factors. The Prostate, 79(14), 1611-1621.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!