2 2 6 6 tetramethyl 4 piperidone 4 oxo temp

HA with a mean molecular weight of 30,000 (polydispersity index of 1.212) was purchased from MRC polysaccharide Corp., Ltd (Toyama, Japan). RPMI 1640 medium, 4-dimethylaminopyridine (DMAP) isoflurane, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Tween 20, sodium dodecyl sulfate (SDS) and zinc acetate were purchased from Wako pure chemical (Osaka, Japan). Protoporphyrin IX (PP) and cetyltrimethylammonium bromide (CTA) were from Sigma-Aldrich Chemical (St. Louis, MO, USA). Water-soluble carbodiimide (WSC) was purchased from Dojindo Laboratory, Kumamoto, Japan. Additionally, 2,2,6,6-Tetramethyl-4-piperidone (4-oxo-TEMP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Esterase from porcine liver was purchased from Roche Diagnostics GmbH (Mannheim, Germany). The other reagents and solvents of a reagent grade were from commercial sources and were used without further purification.

Poly(styrene-co-maleic anhydride) (an SMA copolymer), with a mean molecular weight of 1600 Da, and mTHPC were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 2,2,6,6-Tetramethyl-4-piperidone (4-oxo-TEMP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Other reagents of reagent grade and solvents were purchased from Wako Pure Chemical Industries Ltd. and used without further purification.