Polarization rotation was assessed on a Jasco P-1020 polarimeter (SHIMADZU, Kyoto City, Japan), and Shimadzu UV spectra were determined on a UV-2401A spectrophotometer (SHIMADZU, Kyoto City, Japan). The IR spectra were acquired using a Tenor 27 spectrophotometer (Bruker, Mannheim, Germany). NMR spectra were evaluated on Bruker Avance III 500/600 MHz NMR spectrometers (Bruker, Mannheim, Germany). MS-HRESI were recorded on an Agilent 1290/6540 UPLC instrument/Q-TOF-MS (Agilent, Santa Clara, CA, USA), and semi-preparative HPLC was conducted using the UV-vis detector technology of an Agilent 1200 Series chromatography system (Agilent, Santa Clara, CA, USA) equipped with a YMC-Park ODS-A (250 × 10 mm, S-5 μm) column. Column chromatography (CC) was carried out employing two distinct mesh sizes, 100–200 mesh and 300–400 mesh, RP-18 (40~63 μm, 100 Å), and Sephadex LH-20 (40~70 μm).
Tenor 27 spectrophotometer
Manufactured by Bruker
Sourced in Germany
The Tenor 27 spectrophotometer is a laboratory instrument designed for precise optical measurements. It is capable of measuring the absorbance or transmittance of samples across a range of wavelengths, enabling quantitative analysis of various substances.
Automatically generated - may contain errors
Lab products found in correlation
Uv 2401a spectrophotometer, by Shimadzu (2 mentions)
Sephadex lh 20, by Cytiva (2 mentions)
P 1020 digital, by Horiba (2 mentions)
Uv 2401pc uv visible recording spectrophotometer, by Shimadzu (2 mentions)
Agilent 6200 q tof ms system, by Agilent Technologies (2 mentions)
Dad detector, by Agilent Technologies (2 mentions)
Chromatorex c 18, by Büchi (2 mentions)
P 1020 polarimeter, by Shimadzu (1 mentions)
Agilent 1200 series chromatography system, by Agilent Technologies (1 mentions)
Escalab 250xi, by Thermo Fisher Scientific (1 mentions)
Ultima 4, by Rigaku (1 mentions)
Cary 100 spectrophotometer, by Agilent Technologies (1 mentions)
Sepa 300 polarimeter, by Horiba (1 mentions)
Silica gel, by Qingdao Haiyang Chemical (1 mentions)
Gf254, by Qingdao Haiyang Chemical (1 mentions)
Drx 500, by Bruker (1 mentions)
Am 400, by Bruker (1 mentions)
Avance 3 600, by Bruker (1 mentions)
Mci gel chp 20p, by Mitsubishi Chemical Corporation (1 mentions)
Avance 3 600 mhz spectrometer, by Bruker (1 mentions)
6 protocols using tenor 27 spectrophotometer
1
Spectroscopic Analysis of Chemical Compounds
2
Comprehensive Characterization of Materials
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Transmission electron microscope (TEM) and high resolution transmission electron microscope (HRTEM) micrographs were obtained from FEI Tecnai G2 s-twin transmission electron microscopy with a working voltage of 200 kV. The X-ray photoelectron spectra (XPS) were performed on Thermo Fischer ESCALAB 250Xi. Powder X-ray diffraction (PXRD) measurements were made on the Rigaku Ultima IV diffractometer with Cu-Kα radiation (λ = 0.15405 nm) and a graphite monochromator by scanning over the range of 5–60° at a speed of 0.2° s−1. Fourier transform infrared spectra were measured on a Bruker TENOR 27 spectrophotometer. The UV-visible absorption spectrum was performed on a Cary 100 spectrophotometer (Agilent). All fluorescence measurements were made at room temperature on the EDINBURGH instrument FLS920 equipped with a 980 nm laser.
3
Spectroscopic Characterization of Natural Compounds
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Optical rotations were measured with a Horiba SEPA-300 polarimeter (Horiba, Tokyo, Japan). UV spectra were obtained using a Shimadzu UV-2401A spectrophotometer (Shimadzu, Tokyo, Japan). IR spectra were obtained by a Tenor 27 spectrophotometer (Bruker Optics, Ettlingen, Germany) using KBr pellets. 1D and 2D NMR spectra were carried out on Bruker AM-400, DRX-500, or AVANCE III-600 spectrometers with TMS as an internal standard (Bruker, Karlsruhe, Germany). ESIMS and HRESIMS were run on an Agilent 6530 Q of spectrometer (Agilent, Palo Alto, CA, USA). Column chromatography (CC) was performed using MCI gel (CHP 20P, 75–150 m; Mitsubishi Chemical Corporation, Tokyo, Japan), Polyamide (PA, 80–100 mesh; Sinopharm Chemical Reagent Co., Ltd, Shanghai, China) and silica gel (100–200 or 200–300 mesh; Qingdao Haiyang Chemical Co., Ltd, Qingdao, China). Thin-layer chromatography (TLC) was conducted on silica gel plates GF254 (Qingdao Haiyang Chemical Co., Ltd, Qingdao, China). Fractions were monitored by TLC using various solvent systems, and spots were visualized by spraying improved Dragendorff’s reagent to the silica gel plates or by heating silica gel plates sprayed with 10% H2SO4 in EtOH.
4
Analytical Techniques for Natural Products
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Optical rotations were obtained on a JASCO P-1020 digital polarimeter (Horiba, Kyoto, Japan). UV spectra were recorded on a Shimadzu UV-2401PC UV–visible recording spectrophotometer (Shimadzu, Kyoto, Japan). A Tenor 27 spectrophotometer (Bruker Optics GmbH, Ettlingen, Germany) was used for scanning IR spectroscopy using KBr pellets. 1D and 2D NMR spectra were obtained on Bruker DRX 500 MHz and Avance III 600 MHz spectrometers (Bruker Corporation, Karlsruhe, Germany). HRESIMS were recorded on an Agilent 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) and silica gel (Qingdao Haiyang Chemical Co., Ltd, Qingdao, China) were used for column chromatography (CC). Medium pressure liquid chromatography (MPLC) was performed on a Büchi Sepacore System equipped with pump manager C-615, pump modules C-605 and fraction collector C-660 (Büchi Labortechnik AG, Flawil, Switzerland), and columns packed with Chromatorex C-18 (dimensions 450 mm × i.d. 14 mm, particle size: 40–75 μm, Fuji Silysia Chemical Ltd., Kasugai, Japan). Preparative high performance liquid chromatography (prep-HPLC) were performed on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18 column (particle size 5 μm, dimension 150 mm × i.d. 9.4 mm, flow rate 7 ml min−1, respectively) and a DAD detector (Agilent Technologies).
5
Characterization of Natural Products
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Optical rotations were obtained on a JASCO P-1020 digital polarimeter (Horiba, Kyoto, Japan). UV spectra were recorded on a Shimadzu UV-2401PC UV–Visible recording spectrophotometer (Shimadzu, Kyoto, Japan). A Tenor 27 spectrophotometer (Bruker Optics GmbH, Ettlingen, Germany) was used for scanning IR spectroscopy using KBr pellets. 1D and 2D NMR spectra were obtained on a Bruker Avance III 600 MHz spectrometer (Bruker Corporation, Karlsruhe, Germany). HRESIMS were recorded on an Agilent 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) and silica gel (Qingdao Haiyang Chemical Co., Ltd., Qingdao, China) were used for column chromatography (CC). Medium Pressure Liquid Chromatography (MPLC) was performed on a Büchi Sepacore System equipped with pump manager C-615, pump modules C-605 and fraction collector C-660 (Büchi Labortechnik AG, Flawil, Switzerland), and columns packed with Chromatorex C-18 (dimensions 450 mm × i.d. 14 mm, particle size: 40–75 μm, Fuji Silysia Chemical Ltd., Kasugai, Japan). Preparative high performance liquid chromatography (prep-HPLC) were performed on an Agilent 1260 liquid chromatography system equipped with a Zorbax SB-C18 column (particle size 5 μm, dimensions 150 mm × i.d. 9.4 mm, flow rate 7 ml min−1) and a DAD detector (Agilent Technologies, Santa Clara, CA, USA).
6
Comprehensive Materials Analysis Protocol
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XRD measurements were conducted on a Rigaku SmartLab SE X-ray diffractometer. SEM and EDS data were acquired by a Thermo Fisher Scientific (TFS) Quattro S scanning electron microscope. TEM images and EELS spectra were collected on a field emission JEM-2100F (JEOL, Japan). The HAADF-STEM images and EDS elemental mappings were obtained on a double aberration-corrected Spectra 300 TEM/STEM (TFS). The order of magnitude of the electron dose is down to the 10 3 e -Å -2 . The FT-IR spectra were recorded on a Bruker TENOR 27 spectrophotometer employing the KBr pellets. The product analysis was made using NMR spectroscopy (300 MHz, Bruker AVANCE III BBO Probe) and ultraviolet-visible absorbance spectroscopy (UV-Vis spectrophotometer, SHIMADZU UV-2600). XPS analysis was based on a ESCALAB 220i-XL electron spectrometer from VG Scientific using 300 W Al KR radiation. X-ray absorption spectroscopy (XAS) was conducted in a transmission mode at beamline X-ray absorption fine structure for catalysis (XAFCA) of Singapore Synchrotron Light Source.
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