Necrostatin

Manufactured by Cayman Chemical

Necrostatin is a small molecule compound that functions as a selective inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK1), a protein involved in the regulation of programmed cell death pathways.

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Lab products found in correlation

Metformin, by Cayman Chemical (2 mentions) Phenformin, by Cayman Chemical (1 mentions) Disulfiram, by Cayman Chemical (1 mentions) Ferrostatin, by Cayman Chemical (1 mentions) Bay 876, by Cayman Chemical (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Oligomycin, by Cayman Chemical (1 mentions) Phenformin, by LGC (1 mentions) Aspartate, by Merck Group (1 mentions) Atpenin, by Cayman Chemical (1 mentions) Mg132 s2619, by Selleck Chemicals (1 mentions) Cyclosporine a, by Enzo Life Sciences (1 mentions) 3 bromopyruvate, by Merck Group (1 mentions) Z vad fmk, by Apexbio (1 mentions) Amppcp, by Merck Group (1 mentions) Antimycin a, by Merck Group (1 mentions) Rotenone, by Merck Group (1 mentions) Adenine, by Merck Group (1 mentions)

2 protocols using necrostatin

Cell Culture Conditions for Breast Cancer

Cited 1 time

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MCF7 (from ATCC) and previously established neuT and neuTemt cells [32 (link)] were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin. MCF10DCIS.com cells (obtained from Asterand) and DCIS-Snai1-ER cells [33 (link)] were maintained in DMEM/F12 supplemented with 5% horse serum. Glucose-, pyruvate- and glutamine-free DMEM was obtained from Gibco (catalog A14430). Glucose and glutamine were added to reconstitute low-glucose media. Metformin, phenformin, 2DG, BAY-876, disulfiram, ferrostatin, and necrostatin were purchased form Cayman Chemical.

Liang M., Li J.W., Luo H., Lulu S., Calbay O., Shenoy A., Tan M., Law B.K., Huang S., Xiao T.S., Chen H., Wu L., Chang J, & Lu J. (2022). Epithelial–Mesenchymal Transition Suppresses AMPK and Sensitizes Cancer Cells to Pyroptosis under Energy Stress. Cells, 11(14), 2208.

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Compounds and Reagents for Metabolic Assays

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Compounds dmaKG (dimethyl alpha-ketoglutarate, 349631), deaKG (diethyl alpha-ketoglutarate, CDS008208), etaKG (2-oxo-pentanedioic acid 5-ethyl ester 1-(3-trifluoromethyl-benzyl) ester, SML1743), antimycin A (A8674), rotenone (R8875), aspartate (L-aspartic acid potassium salt, A9381), 2-DG (2-deoxy-D-glucose, 25972), adenine (A2786), ATP (adenosine 5’-triphosphate, A7699), ADP (adenosine 5’-diphosphate, A5285), AMP-PCP (β,γ-methyleneadenosine 5’-triphosphate, M7510), , AOA (aminooxyacetic acid hemihydrochloride, C13408), and 3-bromopyruvate (16490), were purchased from Sigma Aldrich. Oligomycin (11341), necrostatin (11658), atpenin (11898), and metformin (13118) were purchased from Cayman Chemical. 3-NPA (3-nitropropionic acid, 4849) were purchased from Tocris. Cyclosporine A (BMLA1950100) were purchased from Enzo Life Sciences. Z-VAD-FMK (A19025) were purchased from Apexbio Technology. MG-132 (S2619) were purchased from Selleck Chemicals. Phenformin (P29690) were purchased from Toronto Research Chemicals.

Madala H.R., Helenius I.T., Zhou W., Mills E., Zhang Y., Liu Y., Metelo A.M., Kelley M.L., Punganuru S., Kim K.B., Olenchock B., Rhee E., Intlekofer A.M., Iliopoulos O., Chouchani E, & Yeh J.R. (2020). Nitrogen trapping as a therapeutic strategy in tumors with mitochondrial dysfunction. Cancer research, 80(17), 3492-3506.

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