Peripheral blood perfusion (PBP) was analyzed by LASCA (laser speckle contrast analysis—Pericam PSI, Perimed, Jarfalla), in both HCs and SSc patients [19 (link), 20 (link)]. SSc patients were not under the influence of prostanoid intravenous therapy for at least 4 weeks before the registration. After 15 min of acclimatization in the room at a temperature maintained between 20 and 25 °C, PBP was registered on the whole hands, both on the volar and palmar sides, for a total of 4 records of 30 s each one. Setting was set up to perform five images each second and the software automatically measured PBP as perfusion units (P.U.). Subsequently, regions of interest (ROIs) were created as follows: fingertips, periungual areas, palmar and volar aspect of the 2nd–3rd–4th–5th fingers, whole hands. The average PBP values between fingertips, periungual areas, and between right and left hands were measured.
Pericam psi
Manufactured by Perimed
Sourced in Sweden, United States
The PeriCam PSI is a non-invasive imaging system designed to visualize and measure tissue perfusion. It utilizes laser speckle contrast imaging technology to generate real-time perfusion maps of the examined area.
Automatically generated - may contain errors
Lab products found in correlation
Pimsoft, by Perimed (4 mentions)
Vivid 7, by GE Healthcare (1 mentions)
Periflux system 5000, by Perimed (1 mentions)
Rose bengal solution, by Merck Group (1 mentions)
Avertin solution, by Merck Group (1 mentions)
Powerlab, by ADInstruments (1 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
Pimsoft software, by Perimed (1 mentions)
Vms ldf2, by Moor Instruments (1 mentions)
39 protocols using pericam psi
1
Measuring Peripheral Blood Perfusion in Systemic Sclerosis
2
Hind Limb Ischemia Surgery Protocol
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Experimentally induced HLI surgery was performed as described previously.6 , 7 , 9 , 10 Briefly, mice were anesthetized by intraperitoneal injection of a mix of xylazine (5 mg/kg) and ketamine (100 mg/kg), followed by ligation and excision of the femoral artery of the left hind limb. Mice temperatures were kept warm at 37 °C throughout the procedure. The unoperated right hind limb served as nonischemic control, and peripheral blood flow was measured by Laser Speckle Contrast Imaging using PeriCam PSI (Perimed). Data were obtained immediately following surgery (day 0) as well as at day 3, day 7, and day 14 after surgery. The relative changes of blood flow in the hind limbs were expressed as the ratio of the operated to the contra‐lateral hind limb blood flow using the manufacturer's software.
3
Laser Speckle Contrast Analysis of Skin Perfusion
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Blood perfusion was measured as perfusion units (PU) in all patients by LASCA technique (PeriCam PSI, Perimed, Sweden), as previously reported (Ruaro et al., 2014 (link), 2016 (link); Sulli et al., 2014 (link); Lambrecht et al., 2016 (link)), at the level of dorsal and palmar aspect of hands and face, at baseline (W0), after one (W1), four (W4), twelve (W12) and twenty-four (W24) weeks of aminaphtone treatment, and after one (W25) and five (W29) weeks since treatment discontinuation. Raynaud’s condition score (RCS) and both frequency and duration of Raynaud’s attacks were assessed at the same time (see below). For acclimatization, each patient stayed inside the building for a minimum of 15 min before the blood perfusion was examined, at room temperature of about 23°C.
After image recording, different regions of interest (ROIs) were drawn at the fingertip level, periungual areas, dorsum and palm of hands, tip of noise, and whole face (Sulli et al., 2014 (link); Ruaro et al., 2016 (link)). Blood perfusion was measured inside the ROIs (see example inFigure 1 ). The evaluator was blind to both patient treatment and time of visit. For each anatomic area, the average BP was calculated by summing the perfusion values of the two sides, left and right.
All instrumental technical parameters were standardized for all patients and used at follow-up visits.
After image recording, different regions of interest (ROIs) were drawn at the fingertip level, periungual areas, dorsum and palm of hands, tip of noise, and whole face (Sulli et al., 2014 (link); Ruaro et al., 2016 (link)). Blood perfusion was measured inside the ROIs (see example in
All instrumental technical parameters were standardized for all patients and used at follow-up visits.
4
Cerebral Blood Flow Assessment in Rats
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An echocardiograph (Vivid 7, GE Medical Systems, Horten, Norway) equipped with a 12-MHz linear transducer (12L) was used. Data were transferred online to an ultrasound image workstation for subsequent analysis (PC EchoPAC, GE Medical Systems). Ultrasound data comprised Doppler spectral recordings of the right and left CCAs in rats of the BCAS and BCAO groups. Laser Doppler flowmetry (PeriCam PSI, PERIMED AB) was used to detect the CBF. The region of interest in the frontal cortex was consistently set as a circle with 3 mm diameter at 2 mm posterior and 3 mm lateral to bregma. CBF was expressed as the percentage of the baseline level. Ultrasound recordings were repeated in each rat before and 2 h after surgery as well as 1, 3, 7, 14, and 30 days after surgery (n = 6).
5
Cerebral Blood Flow Monitoring in MCAO
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Utilizing the PeriFlux System 5000 (Perimed AB, Järfälla, Sweden), the cerebral blood flow was strictly monitored during the MCAO modeling, the model success was verified, and injury was assessed from the cerebral blood flow measured using a PeriCam PSI (Perimed AB, Järfälla, Sweden) post-operation.
6
Quantifying Cutaneous Blood Flow Responses
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The DBF response was quantified using Laser Speckle Contrast Imaging (LSCI, PeriCam PSI, Perimed, Järfälla, Sweden). During the screening visit, the DBF on the forearm was measured according to the previously described experimental set-up [6, (link)7] (link). For DBF measurements on the fingers, all procedures were conducted after a 30 minutes acclimatization period in semi-recumbent position in a temperature-controlled room of 25±1 °C. Next, nitrile O-shaped rings (7.66 mm inner diameter, Quad Ring BS011 NBR 70 Shore A; Polymax Ltd, Bordon, United Kingdom) were placed on the proximal phalanges of fingers 2, 3 and 4. Thereafter, pre-challenge baseline scans were recorded and subsequently, the challenge agent or vehicle was topically applied within the nitrile ring. Subsequently, the DBF response was recorded every 10 minutes for one hour post-application. The DBF was measured per finger over an area of 3 * 6 cm² and with a distance of 20 ± 1 cm. Five successive recordings, one per second, were averaged. The intensity filter was per default set at 0.14 to 10.00.
Simultaneously with the DBF measurements, subjects reported any cinnamaldehyde-or capsaicininduced pain sensation according to the Numerical Rating Scale-11 (NRS) pain score [6] (link).
Simultaneously with the DBF measurements, subjects reported any cinnamaldehyde-or capsaicininduced pain sensation according to the Numerical Rating Scale-11 (NRS) pain score [6] (link).
7
Rat Model of Ischemic Stroke and Reperfusion
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Cerebral ischemic stroke and reperfusion injury were induced by establishing MCAO/R model [23 (link)]. Fifteen minutes after receiving DADLE or ACSF, the rats were anaesthetized, and their cerebral hemisphere was monitored by laser speckle imaging (PeriCamPSI, PERIMED, Järfälla, Sweden). A monofilament purchased from RWD Life Science (Shenzhen, China) was inserted 18 to 20 mm into the internal carotid to occlude the origin of the MCA. The time interval between drug administration and blood flow of the artery being blocked was averagely 45 min, as the previous studies described [24 (link)]. After 1 h, the monofilament was removed for reperfusion. Before recovering, the rats were placed on a heating plate. The rats in the sham group underwent the same surgical operations except for the monofilament insertion. Statistically, the total mortality was 21% [23 (link),25 (link)].
8
Cerebral Blood Flow Monitoring in Mice
9
Hind Limb Ischemia Surgery in Mice
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Hind limb ischemia surgery was performed as described previously [11 (link),25 (link),26 (link)]. Briefly, mice were anesthetized by intraperitoneal injection of a mix of xylazine (5 mg/kg) and ketamine (100 mg/kg) followed by ligation and excision of the femoral artery of the left hind limb. Mice were kept warm (37 °C) throughout the procedure. The right hind limb served as the non-ischemic control, and peripheral blood flow was measured by Laser Speckle Contrast Imaging using PeriCam PSI (Perimed, United States). Images were obtained immediately following surgery (day 0) as well as at day 3, day 7, day 14, and day 21 after the surgery. The relative changes of blood flow in the GA muscle were expressed as the ratio of the operated to the contra-lateral hind limb blood flow using the manufacturer’s software.
10
Evaluation of Skin Microvascular Blood Perfusion in Systemic Sclerosis
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Skin BP was analyzed by the LASCA technique (Pericam PSI, Perimed, Milan, Italy) at the level of dorsal and palmar aspect of hands and the whole face, in both SSc patients and healthy subjects as previously described (Ruaro et al., 2014 (link), 2016 (link); Sulli et al., 2014 (link)). Different regions of interest (ROIs) were created, as previously reported, i.e., at the level of fingertips, periungual areas, dorsal and palmar aspect of the 3rd finger bilaterally, the dorsum and palm of both hands and face (forehead, tip of nose, zygomas and perioral region) (see Figure 1 for ROI areas) (Sulli et al., 2014 (link); Ruaro et al., 2016 (link), 2018b (link)).
The average BP from either fingertips or periungual areas was calculated by summing the perfusion values of eight fingers together and then dividing the final value by the number of fingers.
The average BP from the two palmar and dorsal areas of the fingers, palm and dorsum of the hands and zygoma was calculated by summing the perfusion values of the two sides (right and left) and then dividing the final value by two. The BP was quantified as perfusion units (PU; Sulli et al., 2014 (link); Ruaro et al., 2016 (link)). The same operator (BR) performed the examination in all PRP, SRP-SSc patients and CNT.
The average BP from either fingertips or periungual areas was calculated by summing the perfusion values of eight fingers together and then dividing the final value by the number of fingers.
The average BP from the two palmar and dorsal areas of the fingers, palm and dorsum of the hands and zygoma was calculated by summing the perfusion values of the two sides (right and left) and then dividing the final value by two. The BP was quantified as perfusion units (PU; Sulli et al., 2014 (link); Ruaro et al., 2016 (link)). The same operator (BR) performed the examination in all PRP, SRP-SSc patients and CNT.
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