U2OS cells that had been transfected with PH-PLCD1-GFP, GFP-vinculin, or mRFP-Lact-C2 and grown on glass cover slips were treated with choline kinase alpha inhibitors EB-3D (10 mM), CK-37(15 mM) or vehicle for 48 h, placed into the imaging chamber (37°C, 5% CO2) of a Zeiss Axio Observer Z.1 Spinning Disk Confocal Microscope and image acquisition was performed with Zen Black software.
Axio observer z 1 spinning disk confocal microscope
Manufactured by Zeiss
The Axio Observer Z.1 Spinning Disk Confocal Microscope is a high-performance microscope system designed for advanced imaging applications. It utilizes a spinning disk confocal technology to provide fast, high-resolution optical sectioning capabilities. The microscope enables the acquisition of clear, detailed images of fluorescently labeled samples.
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Lab products found in correlation
2 protocols using axio observer z 1 spinning disk confocal microscope
1
Imaging choline kinase inhibition
2
Quantifying Colocalization of PI(4,5)P2 and PS
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U2OS cells that had been transfected with PH-PLCD1-GFP and mRFP-Lact-C2 were grown on glass cover slips and treated with choline kinase alpha inhibitors EB-3D (15 mM) or vehicle for 48 h and imaged using Zeiss Axio Observer Z.1 Spinning Disk Confocal Microscope. For each group, confocal images from 25 random cells were quantified for colocalization of PI(4,5)P2 and PS using an imageJ plugin called JaCoP (53 (link)).
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