MDA-MB-231 and MCF-7 were grown in high-glucose DMEM supplemented with 5% fetal bovine serum (FBS, Biowest, Miami, FL, USA), while MDA-MB-436 cells were grown in DMEM/F12 supplemented with 10% FBS (Biowest, Miami, FL, USA).
MCF-10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Biowest, Miami, FL, USA), 20 ng/mL epidermal growth factor (Upstate Biotechnology Incorporated, Lake Placid, NY, USA), 10 µg/mL insulin (Biofluids, Rockville, MD, USA), and 500 ng/mL hydrocortisone.
All media (Biowest, Miami, FL, USA) were also supplemented with 2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin. The cells were maintained in a humidified incubator at 37 °C with 5% CO2.