To measure the clearance of immunocomplexes from the cell surface, Ramos cells (ATCC) were incubated with fluorescein isothiocyanate (FITC)-labeled ASKP1240 or FITC-labeled anti-CD40 agonistic mAb (clone G28.5; ATCC) in RPMI1640 supplemented with 10% fetal bovine serum (FBS) for 15 min at 4°C. The stained cells were washed, and then incubated for varying durations in culture medium at 37°C. At the given time points, cells were removed from the culture and washed with phosphate-buffered saline (PBS) containing 0.1% NaN3 to block internalization. Next, the cells were incubated with phycoerythrin (PE)-labeled anti-human IgG (BioLegend, San Diego, CA) or PE-labeled anti-mouse IgG (BioLegend) for 30 min at 4°C. The geometric mean fluorescence intensity (geoMFI) was measured by flow cytometry and analyzed using CellQuest software (ver. 5.1.1; BD Biosciences, Franklin Lakes, NJ).
Pe labeled anti mouse igg
Manufactured by BioLegend
Sourced in United States
PE-labeled anti-mouse IgG is a flow cytometry reagent that binds to mouse immunoglobulin G (IgG) antibodies. The PE (phycoerythrin) fluorescent dye is conjugated to the anti-mouse IgG antibody, allowing the detection and analysis of mouse IgG-expressing cells using flow cytometry.
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Lab products found in correlation
2 protocols using pe labeled anti mouse igg
1
Clearance of Cell Surface Immunocomplexes
2
SARS-CoV-2 Spike Protein Expression and Binding Analysis
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293T cells were transfected with expression plasmids encoding SARS-CoV-2 S proteins and cultured for 36 h. Cells were digested with trypsin and washed twice with 1 ml of staining buffer (PBS containing 2% FBS). First, cells were incubated with mouse anti-SARS-CoV-2 spike (S2 subunit) antibody (Genetex, 1 μg/ml), neutralizing mAbs (AtaGenix, 0.2 μg/ml), or recombinant hACE2 protein (Sino Biological, Beijing, China; 1 μg/ml) at 4°C for 1 h. After being washed, cells were incubated with Alexa Flour 488-labeled anti-human IgG Fc (BioLegend, San Diego, CA, USA) and/or PE-labeled anti-mouse IgG (BioLegend) secondary antibodies for 1 h. After being washed, the cells were resuspended and analyzed using the Attune™ NxT Flow Cytometer (Thermo Fisher Scientific, Waltham, MA, USA).
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