Merck pre coated silica gel 60 f254 plates

Melting points were determined with an Electrothermal melting point apparatus and are uncorrected. Optical rotations were acquired using a JASCO P-1020 digital polarimeter. IR spectra were recorded on a PerkinElmer FT-IR 400 spectrophotometer. Spectra of solid sample were recorded in the ATR mode. NMR spectra were recorded on a Bruker AVANCE 400 FT-NMR spectrometer, operating at 400 (¹H) and 100 (¹³C) MHz. For the spectra taken in CDCl₃ (δ_H 7.24 and δ_C 77.0) and pyridine-d₅ (δ_H 8.74 and δ_C 150.3), the residual nondeuterated solvent were used as references for ¹H and ¹³C NMR spectra. The electrospray ionization mass spectra (ESIMS) were measured with a Finnigan LC-Q mass spectrometer. High resolution electrospray ionization time-of-flight mass spectra (ESI-TOF-MS) were measured with a Bruker micrOTOF-II mass spectrometer. Column chromatography was carried out using Merck silica gel 60 (<0.063 mm) and reversed phase RP C-18 (40–63 μm, E. Merck). For TLC, Merck precoated silica gel 60 F₂₅₄ plates and RP-TLC, RP-18 F₂₅₄ precoated on aluminium plate (E. Merck) were used. Spots on TLC were detected under UV light and by spraying with anisaldehyde–H₂SO₄ reagent followed by heating. The standard sugars, d(+)-glucose, l(+)-rhamnose and d(+)-xylose, were purchased from Sigma, St. Louis, USA.