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3 protocols using 4 chlorobenzenesulfonate salt did

1

Theranostic Nanoparticles for Alzheimer's

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Inositol was purchased from Shanghai Yi En Chemical Technology Co., Ltd. (Shanghai, China). Oxalyl chloride and Thioflavin-T was obtained from Sigma–Aldrich Co., LLC (USA). DSPE-PEG2000-Mal and DSPE-PEG5000-Mal were purchased from Ponsure Biotechnology (Shanghai, China). DSPE-hyd-DSPE5000-Mal was obtained from Jenkem Technology Co., Ltd. (Beijing, China). PLGA (MW = 3000) was gained from Trueturn-Biological technology Co., Ltd. (Beijing, China). The KLVFFC and C-Acp-Cyclo (CDAGRKQKC) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Aβ1–42 and Aβ42-FITC were obtained from GL Biochem Ltd. (Shanghai, China). Rapamycin was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). 4-Chlorobenzenesulfonate salt (DiD) was gained from Biotium (Hayward, CA, USA). The anti-TAU (phospho S396) and anti-beta amyloid1–42 were purchased from Abcam Ltd. (Cambridge, UK), and the APP polyclonal antibody and phosphorylated TAU antibody [p-TAU (Ser 404)] were purchased from Abclonal (China). ELISA kits (IL-10, IFN-γ and TNF-α) was obtained from BD Pharmingen (USA). Tissue ROS test kit were purchased from BestBio Science (Nanjing, China). The chemical used in this study were analytical grade.
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2

Paclitaxel-Loaded Lipid Nanoparticles for Cancer Therapy

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Paclitaxel was obtained from National Institute for Food and Drug Control. Soybean phospholipids (SPC) was purchased from Kelong Chemical (Chengdu, China). Cholesterol (Chol) was purchased from Bio Life Science & Technology Co., Ltd (Shanghai, China). D-(+)-Biotin was purchased from shanghai darui fine chemical Co., Ltd (Shanghai, China). GOx was obtained from Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(carboxyfluorescein) (CFPE) was purchased from Avanti Polar Lipids (USA). 4'-6-Diamidino-2-phenylindole (DAPI) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Beyotime Institute Biotechnology (Haimen, China). 4-chlorobenzenesulfonate salt (DiD) was purchased from Biotium (USA). Annexin V-FITC/PI apoptosis detection kit was obtained from BD PharmingenTM (USA). Enhanced BCA Protein Assay Kit (Beyotime®, China). Hydrogen Peroxide (H2O2) Assay Kit (Beyotime®, China). Reactive Oxygen Species (ROS) Assay Kit (Beyotime®, China). Calcein-AM/PI Live/Dead Cell Double Dyed Kit (Solarbio®, China). JC-1 dye (Beyotime®, China).
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3

Multifunctional Nanocarrier for Targeted Cancer Therapy

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OVA was purchased from Sigma-Aldrich (St Louis, MO, USA). Polyethyleneimine (PEI, molecular weight 1800 Da) and oleic acid (OA) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). PTX and CQ were purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). 4-Chlorobenzenesulfonate salt (DiD) was provided by Biotium (Hayward, CA, USA). Fluorescein (FITC)-conjugated rat anti-mouse CD4 antibody, PE-conjugated rat anti-mouse CD8a antibody, and the Annexin V-FITC/PI apoptosis detection kit were purchased from BioLegend, Inc. (San Diego, CA, USA). Anti-GAPDH was obtained from CWBIO (Beijing, China), and monoclonal anti-LC3B antibodies were purchased from Sigma-Aldrich. Anti-p62 (SQSTM1) antibodies were from Proteintech Group (Rosemont, IL, USA), and horseradish peroxidase (HRP)-conjugated-goat anti-rabbit IgG (H + L) secondary antibody was from Invitrogen (catalog no. 31460, Carlsbad, CA, USA). FITC-conjugated ImmunoPure goat anti-rabbit IgG (H + L) antibodies were purchased from Feiyi Technology (Wuhan, China), while anti-PD-L1 antibody (atezolizumab) was from Selleck Chemicals (Houston, TX, USA). Mouse mammary breast tumor cells (4T1) and Hela cells were obtained from the Chinese Academy of Science Cell Bank (Shanghai, China).
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