WT mice were injected i.p. with AAI (10 mg/kg) (Sigma) once. Mice in non-nephrotoxic control group were i.p. injected with AAII (10 mg/kg) (Sigma) and control animals received similar volume of PBS.
Aa 2
Manufactured by Merck Group
Sourced in United States
The AA II is a laboratory instrument designed for atomic absorption spectroscopy. It is used to determine the concentration of specific elements in a sample by measuring the absorption of light at specific wavelengths by the sample.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Tianjin Damao (3 mentions)
Magnoflorine, by Merck Group (1 mentions)
Milli q water, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Methanol, by Maclin Biochemical Technology (1 mentions)
Ethylene glycol, by Maclin Biochemical Technology (1 mentions)
Formic acid, by Maclin Biochemical Technology (1 mentions)
Acetonitrile, by Maclin Biochemical Technology (1 mentions)
Quercetin, by Chengdu Pufei De Biotech (1 mentions)
Berberine, by Chengdu Pufei De Biotech (1 mentions)
Chlorogenic acid, by Chengdu Pufei De Biotech (1 mentions)
Acetonitrile acn, by Avantor (1 mentions)
Methanol, by Molar Chemicals (1 mentions)
Chloroform, by Molar Chemicals (1 mentions)
6 protocols using aa 2
1
Nephrotoxicity Induction in Mice
2
Extraction and Analysis of Aristolochic Acids
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AAs are nephrotoxic, mutagenic, and carcinogenic to humans. Thus, they should be treated with caution.All chemicals used were high-performance liquid chromatography grade unless noted otherwise. AA I (90% purity) and AA II (97% purity) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Methanol was supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and Macklin Co., Ltd. (Shanghai, China). Ammonium acetate was purchased from Shanghai Aladdin Co., Ltd. (Shanghai, China). Formic acid was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). Dried A. debilis fruit and lettuce, celery, and tomato seedlings were purchased from the Internet. All water in this experiment was ultrapure water.
3
Analytical Standards Preparation and HPLC
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Analytical standards (magnoflorine, AA-I, and AA-II) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Milli-Q water (Millipore, Bedford, MA, USA) was used for preparing standard solutions, extraction buffers, and eluents. Formic and acetic acids were of high-performance liquid chromatography (HPLC)-grade and obtained from Merck (Darmstadt, Germany). HPLC-grade acetonitrile (ACN) was obtained from PanReac AppliChem (Darmstadt, Germany). All the other chemicals were of analytical-grade.
4
Quantitative Analysis of Acid Standards
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Each acid standard (AAI and AAII) was purchased separately from Sigma-Aldrich. HPLC-grade methanol and acetonitrile were purchased from Merck. The ultrapure water was prepared using the purification with the heal force model Smart Series. The other reagents such as chloroform, ethyl acetate, and methanol were of analytical quality and purchased from Merck.
5
Synthesis and Characterization of Nanoparticle-Analyte Interactions
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AA I and AA II were purchased from Sigma Aldrich (St. Louis, MO). Chlorogenic acid, ursolic acid, quercetin and berberine were from Chengdu Pufei De Biotech Co., Ltd (Chengdu, China). FeCl3·6H2O, sodium acetate anhydrous (NaAc), trisodium citrate, tetraorthosilicate (TEOS), 3-(trimethoxysilyl) propyl methacrylate (TPM), DVB, DMAPAm, 2,2-azobisisobutyronitrile (AIBN) were obtained from Aladdin Industrial Co., Ltd (Shanghai, China). Formic acid, methanol, acetonitrile and ethylene glycol were bought from Shanghai Macklin Biochemical. Co., Ltd (Shanghai, China). The solvents listed above were of chromatographic grade.
6
Phytochemical Analysis of A. clematitis
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AA I and AA II were purchased from Sigma-Aldrich (Budapest, Hungary). The materials and reagents applied in the preparation and analysis of A. clematitis were all of analytical reagent grade of the highest purity available, such as acetonitrile (ACN; VWR Chemicals, Belgium), methanol, hexane, chloroform, ethyl acetate, and butanol (Molar Chemicals, Hungary).
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