Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad) [7 (link)]. Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), ubiquitin (Protein Tech, 10201–2-AP, 1:1000) or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
Mtp11 s
Manufactured by Merck Group
The MTP11-S is a high-quality laboratory equipment product from Merck Group. It serves as a multi-purpose tool for various applications in the scientific research and clinical laboratory settings. The core function of the MTP11-S is to provide a reliable and efficient platform for performing tasks that require precise liquid handling and sample processing. The detailed specifications and intended use of this product are not available for this response.
Automatically generated - may contain errors
Lab products found in correlation
Ab82411, by Thermo Fisher Scientific (3 mentions)
Ab16045, by Abcam (3 mentions)
Abs983, by Santa Cruz Biotechnology (3 mentions)
Sc 365365, by Santa Cruz Biotechnology (3 mentions)
Sc 65882, by Abcam (3 mentions)
Criterion tgx precast protein gel, by Bio-Rad (3 mentions)
Ma5 27915, by Abcam (2 mentions)
Ecl reagent, by PerkinElmer (2 mentions)
Corresponding secondary antibody conjugated to hrp, by GE Healthcare (2 mentions)
3 protocols using mtp11 s
1
Western Blot Analysis of Iron-Regulatory Proteins
2
Western Blot Analysis of Iron Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad)7 (link). Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
3
Western Blot Analysis of Iron Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad)7 (link). Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
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