Titan 320 x ray generator

Manufactured by Shimadzu

Sourced in Japan

The TITAN-320 is an X-ray generator manufactured by Shimadzu. It is designed to produce high-energy X-rays for various analytical applications. The core function of the TITAN-320 is to generate X-rays through the acceleration of electrons and their subsequent collision with a target material.

Automatically generated - may contain errors

Lab products found in correlation

Tas 116, by Active Motif (1 mentions) Streptavidin peroxidase polymer, by Merck Group (1 mentions) S2438, by Merck Group (1 mentions)

5 protocols using titan 320 x ray generator

Radiotherapy and Radiation Effects on Pancreatic Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

When the subcutaneous tumors reached a diameter of approximately 8 mm (5–6 weeks after inoculation for BxPC-3, 8–9 weeks for MIAPaCa-2), RIT and EBRT experiments were conducted. Mice (n = 5 for each dose) bearing a subcutaneous tumor (BxPC-3 or MIAPaCa-2) were injected with 0.74, 1.85, and 3.7 MBq of ⁹⁰Y-TSP-A01 into a tail vein. Protein dose was adjusted to 25 μg for each preparation by adding the intact antibody. As a negative control (0 MBq), five mice were intravenously injected with intact TSP-A01 (25 μg protein/mouse). As a separate experiment, tumors (n = 5 each dose) were irradiated with 0, 15, 30, and 60 Gy of X-rays at a rate of 4.4 Gy/min with a TITAN-320 X-ray generator (Shimadzu, Kyoto, Japan). Other parts of the mouse body were covered with a brass shield to limit unnecessary radiation exposure. The body weight and tumor size were measured at least twice a week for 42 to 49 days. However, when the tumor reached 15 mm in diameter, the mouse was euthanized humanely by isoflurane inhalation. Tumor volume (mm³) was calculated as (length × width²)/2. The tumor volume data were analyzed by two-way repeated measures ANOVA.

Sugyo A., Tsuji A.B., Sudo H., Okada M., Koizumi M., Satoh H., Kurosawa G., Kurosawa Y, & Saga T. (2015). Evaluation of Efficacy of Radioimmunotherapy with 90Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models. PLoS ONE, 10(4), e0123761.

+ Open protocol

+ Expand

Radiation and Gemcitabine Combination Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols

BxPC-3 tumors (n = 5/dose) were irradiated with 0 Gy, 5 Gy, 15 Gy, or 30 Gy of X-rays at a rate of 4.5 Gy/min with a TITAN-320 X-ray generator (Shimadzu, Kyoto, Japan). In the combination treatment with X-ray radiation and gemcitabine, gemcitabine (240 mg/kg body weight) was intravenously administered 24 h before radiation. Body weight and tumor size were measured at least twice a week for 42 days. When the tumor reached 15 mm in diameter, the mouse was euthanized humanely by isoflurane inhalation. Tumor volume (mm³) was calculated as (length × width²)/2. The tumor volume data were normalized by the volume at day 0 and analyzed by two-way repeated-measures analysis of variance.

Sugyo A., Tsuji A.B., Sudo H., Koizumi M., Ukai Y., Kurosawa G., Kurosawa Y., Saga T, & Higashi T. (2018). Efficacy Evaluation of Combination Treatment Using Gemcitabine and Radioimmunotherapy with 90Y-Labeled Fully Human Anti-CD147 Monoclonal Antibody 059-053 in a BxPC-3 Xenograft Mouse Model of Refractory Pancreatic Cancer. International Journal of Molecular Sciences, 19(10), 2979.

+ Open protocol

+ Expand

TAS-116 Radiosensitization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

TAS-116 (Supplementary figure S1) was purchased from Active Biochem (Maplewood, NJ) and dissolved into dimethyl sulfoxide (DMSO). Cells were pretreated with TAS-116 or DMSO for 24 hr, and irradiated with X rays or carbon ions. Cells were irradiated with a TITAN-320 X-ray generator (200 kV, 20 mA, Shimadzu, Kyoto, Japan) or with 290 MeV/n carbon ions (6 cm spread-out Bragg peak (SOBP), ~50 keV/μm) accelerated by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at the National Institute of Radiological Sciences (NIRS). After irradiation, cell culture medium was replaced with fresh medium without drug.

Lee Y., Sunada S., Hirakawa H., Fujimori A., Nickoloff J.A, & Okayasu R. (2016). TAS-116, a novel Hsp90 inhibitor, selectively enhances radio-sensitivity of human cancer cells to X-rays and carbon ion radiation. Molecular cancer therapeutics, 16(1), 16-24.

+ Open protocol

+ Expand

Tumor Irradiation and Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

When subcutaneous tumors reached a diameter of approximately 8 mm, tumors were irradiated with 30 Gy of X-rays at a rate of 3.9 Gy/min with a TITAN-320 X-ray generator (Shimadzu, Kyoto, Japan). Other parts of the mouse body were covered with a brass shield. On post-exposure days 1, 3, and 7, tumors (n = 3 per time point) were sampled and fixed in 10% (v/v) neutral buffered formalin and embedded in paraffin for sectioning. Nontreated tumors were used as controls. Sections (thickness, 1 μm) were immunostained with antibody 3–6 (diluted 1:200) followed by horseradish peroxidase-conjugated anti-rat immunoglobulin from a kit (BD, Franklin Lakes, NJ, USA). Nuclei were counterstained with hematoxylin.
To compare antibodies 3–6 and 12–2–7, six subcutaneous A375 tumors were fixed with 4% paraformaldehyde overnight in 0.1 M sodium phosphate (pH 7.2). After dehydration in ethanol, the tissues were embedded in polyester wax. Adjacent sections were immunostained with anti-TNC antibody 3–6 (6 μg/mL) or 12–2–7 (8 μg/mL) as the primary antibody; the secondary antibody was goat anti-rat IgG biotin conjugate (SC-2041, Santa Cruz Biotechnology, Dallas, TX, USA; diluted 1:1000). A streptavidin-peroxidase polymer (S2438, Sigma; diluted 1:1000) served as the detection reagent. Coloring was done with diaminobenzidine, and nuclei were stained with methyl green.

Sugyo A., Tsuji A.B., Sudo H., Takano K., Kusakabe M, & Higashi T. (2020). Proof of Concept Study for Increasing Tenascin-C-Targeted Drug Delivery to Tumors Previously Subjected to Therapy: X-Irradiation Increases Tumor Uptake. Cancers, 12(12), 3652.

+ Open protocol

+ Expand

Tumor Irradiation and Growth Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

When H226 tumor volumes reached approximately 80 mm³, tumors (n = 5/dose) were irradiated with 0, 25, and 50 Gy of X‐rays at a rate of 4.0 Gy/min using a TITAN‐320 X‐ray generator (Shimadzu, Kyoto, Japan). Other parts of the mouse body were covered with a brass shield to limit unnecessary radiation exposure. Tumor size and body weight were measured at least twice a week for 8 weeks after irradiation, and tumor volume was calculated using the formula mentioned above. When the tumor volume reached greater than 800 mm³, the mouse was killed humanely by isoflurane inhalation.

Sudo H., Tsuji A.B., Sugyo A., Saga T., Kaneko M.K., Kato Y, & Higashi T. (2019). Therapeutic efficacy evaluation of radioimmunotherapy with 90Y‐labeled anti‐podoplanin antibody NZ‐12 for mesothelioma. Cancer Science, 110(5), 1653-1664.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!