H1650

LUAD cell lines A549 (BNCC337696), PC-9 (BNCC340767), H1975 (BNCC340345), H1650 (BNCC100260), and human normal bronchial epithelial cell line BEAS-2B (BNCC338205) were all purchased from BeNa Culture Collection (Beijing, China). All cell lines were cultured in 100 U RPMI-1640 mediums supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin (Invitrogen, Grand Island, NY, USA), and 100 μg/ml streptomycin (Invitrogen, Grand Island, NY, USA), and maintained in an incubator with 5% CO₂ at 37°C.

LUAD cell lines H1650 (BNCC100260), Calu-3 (BNCC338514), A549 (BNCC337696), H1975 (BNCC100301), and human bronchial epithelial cell line BEAS-2B (BNCC338205) were bought from BeNa Culture Collection. All cells were kept in Roswell Park Memorial Institute (RPMI)-1640 (Thermo Fisher Scientific Company, Waltham, Massachusetts, USA) medium containing 5% fetal bovine serum (FBS), and cultured in an incubator under general conditions.
NC mimic, miR-30a-5p mimic (mimic) were offered by GenePharma (Shanghai, China). oe-CTHRC1 vector, 3 si-CTHRC1 vectors and their negative control lentivirus packing vectors were acquired from Invitrogen (Carlsbad, CA, USA). Vectors were transiently transfected into Calu-3 cells with Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). All cells were cultivated for at least 24 h in the complete medium before transfection, and collected after 36–48 h of transfection.

NSCLC cell lines (H1650, PC9, H1299, and A549) and normal human bronchial epithelial cell line (HBE) were obtained from BeNa Culture Collection (Beijing, China). These cell lines were both cultured with Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Gibco, Carlsbad, CA, United States) supplemented with heat-inactivated 10% fetal bovine serum (FBS) and 10% penicillin (100 U/mL)/streptomycin (100 μg/mL) in a 37°C and 5% CO₂ incubator.