Bs 1103r

Manufactured by Bioss Antibodies

The Bs-1103R is a piece of lab equipment designed for centrifugation. It has a maximum speed of 3,000 rpm and a maximum relative centrifugal force of 1,103 x g. The centrifuge can accommodate a variety of sample tubes and microplates.

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Lab products found in correlation

Bs 10699r, by Bioss Antibodies (1 mentions) Ifn β, by Bioss Antibodies (1 mentions) Pd l1, by Bioss Antibodies (1 mentions)

2 protocols using bs 1103r

Comprehensive Immunohistochemical Analysis of Tumor Microenvironment

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All immunohistochemistry (IHC) staining was performed at the Boyuan Biological Laboratory. Whole section slides of FFPE tumor and paratumor were stained using a Dako instrument with PD‐L1 (bs‐1103R, Bioss, 1:200 dilution), IDO (13268‐1‐AP, Proteintech, 1:200 dilution), CD8(bs‐10699R, Bioss, 1:200 dilution), TIM‐3 (bs‐10699R, Bioss, 1:200 dilution) and FOXP3+ (bs‐10699R, Bioss, 1:200 dilution) antibodies. Whole section slides of FFPE lymph node were stained on a Dako instrument with PD‐L1 (bs‐1103R, Bioss, 1:200 dilution), IDO (13268‐1‐AP, Proteintech, 1:200 dilution) antibodies. All indices including PD‐L1, IDO, TIM‐3, CD8+, and FOXP3+ were stained Individually with corresponding antibody. The final staining results of the target protein were seen as a brown color, and the nucleus was blue‐purple.

Zhang S., Cai H., Huang J, & Wang G. (2022). Impact of the combination of sintilimab and chemotherapy on the tumor and paratumor PD‐L1, IDO, TIM‐3, FOXP3+ and CD8 expressions in patients with advanced esophageal squamous cell carcinoma. Thoracic Cancer, 13(23), 3284-3294.

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Comprehensive Immunohistochemical Profiling

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Pre- and post-treatment tissue specimens were stained for STING (Cell signaling technology, #13647S, 1:100), PD-L1 (Bioss, bs-1103R, 1:300), IFN-β (Bioss, bs-23731R, 1:300), CD3 (Proteintech, 17617-1-AP, 1:100), CD8 (Proteintech, 66868-1-Ig, 1:1000). 5-um-thick tissue sections were deparaffinized in xylene, passed through graded alcohols, and antigen repaired with citrate buffer (pH=7.6) in a steam pressure cooker. The sections were blocked with 5% BSA 1H and incubated with primary antibodies at 4°C overnight, and then washed in 50 mM Tris-HCl, pH 7.4 and incubated with horseradish peroxidase-conjugated secondary antibodies. Immunoperoxidase staining was performed with DAB system. Slides were counterstained with haematoxylin. Immunofluorescence-stained sections were added dropwise with DAPI, incubated for 10 minutes and then rinsed 3 times with PBS. Immunohistochemical sections were dehydrated in in graded alcohol and xylene, and sealed with neutral gum. Immunostaining sections were sealed with glycerin.

Gao H., Zhang X., Ren M., Jiang A., Liu N., Wang J., Zheng X., Liang X., Ruan Z., Tian T., Fu X, & Yao Y. (2024). Platinum-based neoadjuvant chemotherapy upregulates STING/IFN pathway expression and promotes TILs infiltration in NSCLC. Frontiers in Oncology, 14, 1346225.

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