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Ab77822

Manufactured by Abcam
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Ab77822 is a monoclonal antibody that recognizes the human Integrin alpha V protein. The antibody is suitable for use in various immunoassay applications.

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Lab products found in correlation

Ab5790, by Abcam (1 mentions) Ab97959, by Abcam (1 mentions) Ab15894, by Merck Group (1 mentions) Enhanced chemiluminescence ecl western blotting system, by Thermo Fisher Scientific (1 mentions) Zinquin, by Merck Group (1 mentions) T6793, by Merck Group (1 mentions) T5168, by Merck Group (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Ab150078, by Abcam (1 mentions) Ab150105, by Abcam (1 mentions) Odyssey, by LI COR (1 mentions) Ipvh00010, by Merck Group (1 mentions) Mab374, by Merck Group (1 mentions)

4 protocols using ab77822

1

Antibody Characterization for Neurodevelopment

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Primary antibodies for β-actin (#12620), phospho (Thr202/Tyr204) ERK (#4370), and ERK (#9102), were from Cell Signaling Technology (Danvers, MA, USA). Antibodies for glutamic acid decarboxylase 65 (GAD65; SC-377154) and Tbr1 (SC-376258; Western blot) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for Neu-N (MAB377) and Tbr2 (AB15894) were from Millipore (Burlington, MA, USA). The antibody for Ki67 (550609) was obtained from BD Pharmingen (San José, CA, USA). Antibodies for Sox2 (ab97959), Tbr1 (ab31941; immunofluorescence), vGlut1 (ab77822) and Pax6 (ab5790) were from Abcam Inc. (Cambridge, MA, USA). Secondary fluorescent antibodies were obtained from Jackson ImmunoResearch Co. Laboratories (West Grove, PA, USA). Polyvinylidene difluoride (PVDF), membranes and molecular weight standards for Western blot were obtained from BIO-RAD (Hercules, CA, USA). The enhanced chemiluminescence (ECL) Western blotting system was from Thermo Fisher Scientific Inc. (Piscataway, NJ, USA). Zinquin, the antibody for γ amino butyric acid (GABA; A2052), and all other reagents were of the highest quality available and were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
Adamo A.M., Liu X., Mathieu P., Nuttall J.R., Supasai S, & Oteiza P.I. (2019). Early Developmental Marginal Zinc Deficiency Affects Neurogenesis Decreasing Neuronal Number and Altering Neuronal Specification in the Adult Rat Brain. Frontiers in Cellular Neuroscience, 13, 62.
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2

Antibody Usage for Xenopus and Cell Lines

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An affinity-purified goat antibody against recombinant mouse KATNAL2 (accession number LN831865) (Ververis et al., 2016 (link)) was used at 1:100 for Xenopus embryos or at 1:75 for XL177 cells. An affinity-purified rabbit antibody against recombinant mouse KATNAL2 (accession number LN831865) was custom-made by Davids Biotechnologie (Regensburg, Germany) and used at 1:100 for embryos and 1:200 for cells. A mouse monoclonal antibody (mab) against acetylated-tubulin (T6793, Sigma) was used at 1:700 for embryos and 1:500 for cells and a mab against α-tubulin (T5168, Sigma) was used at 1:5000 for cells. Mouse antibodies were used against β-tubulin (DSHB, E7, 1:100), PCNA (Life Technologies, PC10, 1:100), and poly-glutamylation (AdipoGen, 1:100). A rabbit antibody was used against vGLUT1 (Abcam, ab77822, 1:100). Primary antibodies were used in conjunction with appropriate fluorescently labeled Alexa-Fluor secondary antibodies (Thermo Fisher Scientific).
Willsey H.R., Walentek P., Exner C.R., Xu Y., Lane A.B., Harland R.M., Heald R, & Santama N. (2018). Katanin-like protein Katnal2 is required for ciliogenesis and brain development in Xenopus embryos. Developmental biology, 442(2), 276-287.
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3

High-throughput Whole-mount Immunostaining of Tadpoles

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Tadpoles were fixed in 4% paraformaldehyde, bleached, and stained whole-mount according to (H. R. Willsey et al. 2018 (link)). These stainings were done in a high-throughput basket format to accommodate many samples in parallel (Sive, Grainger, and Harland 2007), but with a coarser mesh (200 μm) and 3D-printed racks (3D printer files are available at willseyfroggers.org/resources). Primary antibodies used were against β-Tubulin (DSHB, E7, 1:100); PCNA (PC10, Life Technologies, 1:50); vGLUT1 (Abcam, ab77822, 1:100),.
Rankin Willsey H., Exner C.R., Xu Y., Everitt A., Sun N., Wang B., Dea J., Schmunk G., Zaltsman Y., Teerikorpi N., Kim A., Anderson A.S., Shin D., Seyler M., Nowakowski T.J., Harland R.M., Willsey A.J, & State M.W. (2021). Parallel in vivo analysis of large-effect autism genes implicates cortical neurogenesis and estrogen in risk and resilience. Neuron, 109(5), 788-804.e8.
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4

Quantitative Western Blot Analysis

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20ug of cell lysates were prepared using IP Lysis Buffer (Pierce, 87787) plus protease inhibitor (Roche, 04693124001) and phosphatase inhibitor mixtures (Roche, 04906845001), proteins were separated by polyacrylamide gels (Novex, NP0323BOX) containing 0.1% SDS (SDS-PAGE), and transferred to PVDF membranes (Millipore, IPVH00010). Membranes were blocked overnight in 5% milk at 4°C. Immunoblots were probed for 2 hr at room temperature in 5% milk/TBST with the following primary antibodies: Anti-VGluT1 antibody (Abcam, ab77822) (VGluT1 is an alias of SLC17A7) at 1ug/ml, and anti-GAPDH (Millipore, MAB374) at 1ug/ml. Immunoblots were washed 3x for 10′ in TBST. Anti-rabbit (Abcam, ab150078) and anti-mouse (Abcam, ab150105) were used for 1 hr at room temperature and diluted 1:2000 in 5% milk/TBST. Immunoblots were washed 3x for 10′ in TBST, dried at RT, and visualized using a LI-COR Odyssey.
Lin B., Lee H., Yoon J.G., Madan A., Wayner E., Tonning S., Hothi P., Schroeder B., Ulasov I., Foltz G., Hood L, & Cobbs C. (2015). Global analysis of H3K4me3 and H3K27me3 profiles in glioblastoma stem cells and identification of SLC17A7 as a bivalent tumor suppressor gene. Oncotarget, 6(7), 5369-5381.
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