Rediject d luciferin ultra bioluminescence substrate

The luminescence intensity of the DsRed-Luciferase-miPSC-derived cardiac tissue constructs was measured using the Luciferase Assay System (Promega) in vitro. BLI was performed using the IVIS Lumina II (PerkinElmer). Six minutes prior to measurement of luminescence, 150 mg/kg body weight of Rediject D-Luciferin Ultra Bioluminescence Substrate (PerkinElmer) was injected intraperitoneally. The integration time was fixed at 5 minutes for each image. All images were analyzed with Living Image Software (PerkinElmer).

Graft survival was monitored at day 1, 4, 7, 10, and 14 by bioluminescence imaging on an IVIS Lumina II (Perkin Elmer, Waltham, MA). In brief, Rediject D-Luciferin Ultra Bioluminescence Substrate (Perkin Elmer) was administered intraperitoneally at 150 mg/kg body weight 6 minutes prior to imaging. Animals were then placed in a light-tight chamber, and photons emitted from luciferase-expressing cells were collected for five minutes per image. Images were analyzed in Living Image Software (PerkinElmer). To verify the correlation between luminescence intensity and number of iPSC-derived cardiomyocytes, cells were incubated for 24 hours in 96-well plates containing DMEM and 15% FBS, and assayed using a luciferase assay system (Promega) on a Synergy HT microplate reader equipped with an injector and with time-resolved fluorescence capability (BioTek).

Transplanted cell survival was monitored at days 1, 7, and 10 days with BLI using IVIS Lumina II (PerkinElmer, Waltham, MA). In brief, 6 min prior to measurement of luminescence, Rediject D-Luciferin Ultra Bioluminescence Substrate (PerkinElmer) was administered intraperitoneally at a dose of 150 mg/kg body weight. Animals were placed in a light-tight chamber, and photons emitted from luciferase-expressing cells were collected with an integration time of 5 min for each image. All images were analyzed with Living Image Software (PerkinElmer).