Tissue was collected and fixed in 10% buffered formalin. The sample was then embedded in paraffin and sliced into 5-μm-thick sections. Hematoxylin and eosin (HE) staining was performed and analyzed by microscopy. Immunohistochemistry was performed using the primary antibodies for PPARα and TRPV1 (Proteintech). Frozen sections (8μm) were used for Oil Red O staining.
Trpv1
Manufactured by Proteintech
TRPV1 is a protein that functions as a calcium-permeable cation channel. It is responsive to temperature, protons, and various chemical stimuli. TRPV1 plays a role in the detection and transmission of noxious thermal and chemical stimuli.
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Lab products found in correlation
2 protocols using trpv1
1
Histological and Immunohistochemical Analysis
2
Spinal Cord Immunofluorescence Staining
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The mice in the four groups were anesthetized with 2% isoflurane. A solution of 0.9% saline and 4% paraformaldehyde was used to perfuse the mice. The L4-L6 spinal cord was removed and fixed. 25 μM spinal cord sections were cut on a cryo-thermostat for immunofluorescence staining. After rinsing with phosphate-buffered normal saline (PBS), sections were permeated with 0.3% Triton X-100. They were then sealed with 10% normal goat serum for 2 h and incubated with primary antibodies overnight. The primary antibody markers were TRPV1(1:300, Proteintech Group, Mouse, No. 66983-1-Ig.), NMDAR2B (1:100; Abcam, Rabbit. No. Ab565783), Ionized calcium-binding adapter molecule 1 (IBA-1;1:200; Abcam, Goat. No. Ab5076), and glial fibrillary acidic protein (GFAP; 1:200; Sigma, Mouse. No. G6171). 4,6-diamino-2-phenyl indole (DAPI; 5 µg/ml; Sigma- Aldrich) was used to label the nuclei. The sections were then incubated for 2 h with Alexa Fluor 350 and 488 secondary antibodies. Finally, images were captured using a Zeiss LSM510 confocal microscope (Zeiss, Thornwood, CA, USA).
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