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P foxo1 3

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P-FoxO1/3 is a phospho-specific antibody that recognizes phosphorylated forms of the FoxO1 and FoxO3 transcription factors. The antibody is designed for use in various laboratory techniques, such as Western blotting, to detect and analyze the phosphorylation status of these proteins.

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Lab products found in correlation

P raptor, by Cell Signaling Technology (2 mentions) P ampkα, by Cell Signaling Technology (2 mentions) Anti actin c4, by Abcam (2 mentions) Compound c, by Enzo Life Sciences (2 mentions) P ndrg1, by Cell Signaling Technology (2 mentions) Sodium palmitate, by Nu-Chek Prep (2 mentions) P acc1 ser79, by Cell Signaling Technology (2 mentions) P akt, by Cell Signaling Technology (2 mentions) Anti flag m2, by Merck Group (2 mentions) Ndrg1, by Cell Signaling Technology (2 mentions) Ampkα, by Cell Signaling Technology (2 mentions) P 4ebp1, by Cell Signaling Technology (2 mentions) Ab54477, by Abcam (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) P irs 1, by Merck Group (1 mentions) Pan akt, by Cell Signaling Technology (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ripa buffer, by Merck Group (1 mentions)

Spelling variants of this product

FOXO1 (243 citations) P-FoxO1 (58 citations)

3 protocols using p foxo1 3

1

Compound C and Sodium Palmitate Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Compound C was obtained from Enzo Life Sciences. Sodium Palmitate is from NU-CHEK Prep Inc. For western blotting, anti-Flag M2 (RRID:AB_259529) antibody was purchased from Sigma, antibodies against SGK1 (RRID:AB_2687476), SGK2 (RRID:AB_10828732), SGK3 (RRID:AB_10949507), HSP90 (RRID:AB_2233307), Akt (RRID:AB_915783), p-Akt (Thr308) (RRID:AB_2255933), p-Akt (Ser473) (RRID:AB_2315049), NDRG1 (RRID: AB_11140640), p-NDRG1(Thr346) (RRID:AB_10693451), FoxO1 (RRID:AB_2106495), p-FoxO1/3 (Thr24/32) (RRID:AB_2106814), S6K (RRID:AB_390722), p-S6K (Thr389) (RRID:AB_2269803), S6 (RRID:AB_331355), p-S6 (S240/244) (RRID:AB_10694233), 4EBP1 (RRID:AB_2097841), p-4EBP1 (Thr37/46) (RRID:AB_560835), p-4EBP1 (Ser65) (RRID:AB_330947), AMPKα (RRID:AB_10624867), p-AMPKα (Thr172) (RRID:AB_331250), p-AMPKα(Ser485/491) (RRID:AB_331250), ACC1 (RRID:AB_2219397), p-ACC1 (Ser79) (RRID:AB_330337), Raptor (RRID:AB_561245)and p-Raptor (Ser792) (RRID:AB_2249475) were obtained from Cell Signaling Technology. Anti-actin (C4) was obtained from Abcam.
Zhou B., Zhang Y., Li S., Wu L., Fejes-Toth G., Naray-Fejes-Toth A, & Soukas A.A. (2021). Serum- and glucocorticoid-induced kinase drives hepatic insulin resistance by directly inhibiting AMP-activated protein kinase. Cell reports, 37(1), 109785.
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2

Insulin Signaling Pathway Protein Analysis

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Tissue fragments and cells were homogenized in RIPA buffer (EMD
Millipore) supplemented with protease and phosphatase inhibitors (Biotool).
Lysates were separated from insoluble material by centrifugation (12,000
RPM, 15 min, 4°C) and total protein content was determined by BCA
assay (ThermoFisher). Equal protein ammounts (~10 μg) were
resolved by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF)
membranes (EMD Millipore). Membranes were immunoblotted with the indicated
antibodies: p-IR/IGF1R (#3024, Cell Signaling), IRβ (sc-711, Santa
Cruz), IGF1Rβ (#3027, Cell Signaling), p-IRS1 (09–432,
Millipore), IRS1 (611394, BD), p-AktS473 (#9271, Cel Signaling),
Akt pan (#4685, Cell Signaling), p-FoxO1/3 (#9464, Cell
Signaling), FoxO1 (#9454, Cell Signaling), p-ERK1/2 (#9101, Cell
Signaling), ERK1/2 (#9102, Cell Signaling), CPT1A (ab128568,
Abcam), HADHA (ab54477, Abcam), SREBP1 (sc-8984, Santa Cruz), Fatty Acid
Synthase (ab22759, Abcam), Vinculin (#3574, Chemicon).
Batista T.M., Garcia-Martin R., Cai W., Konishi M., O’Neill B.T., Sakaguchi M., Kim J.H., Jung D.Y., Kim J.K, & Kahn C.R. (2019). Multi-dimensional Transcriptional Remodeling by Physiological Insulin In vivo. Cell reports, 26(12), 3429-3443.e3.
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3

Compound C and Sodium Palmitate Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Compound C was obtained from Enzo Life Sciences. Sodium Palmitate is from NU-CHEK Prep Inc. For western blotting, anti-Flag M2 (RRID:AB_259529) antibody was purchased from Sigma, antibodies against SGK1 (RRID:AB_2687476), SGK2 (RRID:AB_10828732), SGK3 (RRID:AB_10949507), HSP90 (RRID:AB_2233307), Akt (RRID:AB_915783), p-Akt (Thr308) (RRID:AB_2255933), p-Akt (Ser473) (RRID:AB_2315049), NDRG1 (RRID: AB_11140640), p-NDRG1(Thr346) (RRID:AB_10693451), FoxO1 (RRID:AB_2106495), p-FoxO1/3 (Thr24/32) (RRID:AB_2106814), S6K (RRID:AB_390722), p-S6K (Thr389) (RRID:AB_2269803), S6 (RRID:AB_331355), p-S6 (S240/244) (RRID:AB_10694233), 4EBP1 (RRID:AB_2097841), p-4EBP1 (Thr37/46) (RRID:AB_560835), p-4EBP1 (Ser65) (RRID:AB_330947), AMPKα (RRID:AB_10624867), p-AMPKα (Thr172) (RRID:AB_331250), p-AMPKα(Ser485/491) (RRID:AB_331250), ACC1 (RRID:AB_2219397), p-ACC1 (Ser79) (RRID:AB_330337), Raptor (RRID:AB_561245)and p-Raptor (Ser792) (RRID:AB_2249475) were obtained from Cell Signaling Technology. Anti-actin (C4) was obtained from Abcam.
Zhou B., Zhang Y., Li S., Wu L., Fejes-Toth G., Naray-Fejes-Toth A, & Soukas A.A. (2021). Serum- and glucocorticoid-induced kinase drives hepatic insulin resistance by directly inhibiting AMP-activated protein kinase. Cell reports, 37(1), 109785.
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