Slc17a6 ires cre

All animal procedures followed the ethical guidelines outlined in the NIH Guide for the Care and Use of Laboratory Animals, and all protocols were approved by the Institutional Animal Care and Use Committee at Harvard Medical School. Animals were maintained under constant temperature (23 ± 1 °C) and relative humidity (46 ± 5%) with a 12-h light–dark cycle. Mice used in this study were from both sexes and of a mixed genetic background. Glp1r-ires-cre (029283), Gpr65-ires-cre (029282) and Crhr2-ires-cre (033728) mice were previously made in the laboratory and are now available at the Jackson Laboratory. Slc17a6-ires-cre (Vglut2-ires-cre, 028863), Slc32a1-ires-cre (Vgat-ires-cre, 028862), Rosa26-lsl-Gfp-L10a (024750), Tac1-ires-cre (021877), Pdyn-ires-cre (027958), Penk-ires-cre (025112), Cartpt-ires-cre (028533), Sst-ires-cre (013044), Th-cre (021877) and Gcg-cre (030542) mice were purchased from the Jackson Laboratory.

Non-transgenic C57Bl/6 mice were obtained from Charles River (Manston, Kent, UK), Envigo (Huntingdon, UK and Indianapolis IN) or Janvier Labs (Le Genest-Saint-Isle, France). Pomc^eGFP, Cck^ires-Cre, Crh^ires-Cre (Taniguchi et al., 2011 (link)), Slc17a6^ires-Cre and Rosa26-loxSTOPlox-eYFP were all purchased from Jackson Laboratories (stock numbers 009593, 012706, 012704, 016963 and 006148, respectively; Bar Harbor, ME). We have described the generation of the PPG (Gcg^Cre) and PrRP (Prlh^ires-Cre) mice (Parker et al., 2012 (link); Dodd et al., 2014 (link)). Calca^ires-Cre mice (Carter et al., 2013 (link)) were a kind gift from Prof Richard Palmiter (Howard Hughes Medical Institute, University of Washington).

Animals were housed at 22–24°C on a 12:12 light/dark cycle (light cycle: 6:00 a.m. to 6:00 p.m.) with standard mouse chow (Teklad F6 Rodent Diet 8664; Harlan Teklad) and water provided ad libitum, unless specified otherwise. Adult male and female mice (8–16 weeks old) were used for experiments. Mice were maintained on a mixed background. Transgenic mouse strains used: Avp-IRES-Cre (Pei et al., 2014 (link)), Slc17a6-IRES-Cre (Vglut2-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs Stock 016963), Slc32a1-IRES-Cre (Vgat-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs stock 016962), Nos1-IRES-Cre (Jackson Labs Stock 017526), Slc17a6-IRES-Flp (Vglut2-IRES2-FLPo-D) (Jackson Labs Stock 030212), Slc32a1-IRES-Flp (Vgat-IRES2-FLPo-D) (Jackson Labs Stock 031331), Th-Cre (Savitt et al., 2005 (link)) (Jackson Labs Stock 008601), Dbh-Flp (MMRRC 041575), Avp-GFP (MMRRC 015858-UCD), Agrp-IRES-Cre (Tong et al., 2008 (link)) (Jackson Labs Stock 012899), and Pomc-IRES-Cre (Fenselau et al., 2017 (link)).