Peripheral blood samples were collected from all participants in the present translational study. Samples were collected into Vacutainer® Rapid Serum Tubes (BD Biosciences, Franklin Lakes, NJ, USA) containing silica clot activator polymer gel at baseline in the morning on days 1 or 0 of the first cycle of chemotherapy (n=73) and prior to the second cycle of chemotherapy (n=37), or before starting a new line of salvage chemotherapy in patients with relapsed disease (n=10). Patients' blood samples (1 ml) were centrifuged at 2,800 × g for 10 min to separate the serum from the blood cells. Serum aliquots were stored at −80°C until further analysis.
Vacutainer rapid serum tube
Manufactured by BD
Sourced in United States, Canada
The BD Vacutainer Rapid Serum Tube is a blood collection tube designed for the rapid separation of serum from whole blood samples. It contains a serum separator gel that facilitates the separation of serum from the cellular components of the blood during centrifugation.
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Lab products found in correlation
11 protocols using vacutainer rapid serum tube
1
Serum Sampling and Storage for Chemotherapy
2
Bacterial Infection Detection in Animals
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When animals were sacrificed, blood was collected using Vacutainer rapid serum tubes (RST) (BD Diagnostics, Franklin Lakes, NJ; catalog number 368774). Organs were harvested and homogenized in PBS. The presence of bacterial infection was examined by culturing the blood or tissue lysates on nutrient agar plates for overnight at 37°C. Numbers of colony formation were normalized with the volume of blood or with the amount of protein in tissue lysates.
3
Standardized Blood Sampling Protocol
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Ten minutes before (baseline) and at several timepoints after (0, 30, 90, 180, and 270 min post-exercise) the standardized incremental tests on visits 2 and 3, venous blood was drawn from an antecubital vein into 6 ml BD vacutainer tubes spray-coated with EDTA anti-coagulant for MNC isolation and 5 ml BD vacutainer rapid serum tubes with thrombin and a gel barrier (Becton Dickinson AG, Allschwil, Switzerland) for serum analysis. Throughout the duration of blood collection, participants remained in the laboratory conducting light office work or reading. Until completion of the last blood withdrawal, they were not allowed to shower, eat, or drink except for a standardized meal provided 2 h after exercise cessation and water which participants had access to ad libitum (consumed amount was reported in a log file).
All blood samples were processed within 3.5 h from timepoint of withdrawal. Serum was isolated via centrifugation and stored at −80°C until further analysis of TOC and TAC capacities. Human peripheral blood MNCs were isolated via density gradient centrifugation using Ficoll Histopaque (Ficoll-Paque™ Plus; GE Healthcare, Opfikon, Switzerland). Differential leukocyte counts were obtained in duplicate by a hematology analyzer (Beckman Coulter AC. T diff; Beckman Coulter, Fullerton, United States; intra-sample coefficient of variation for MNC counts = 3.05%, n = 23).
All blood samples were processed within 3.5 h from timepoint of withdrawal. Serum was isolated via centrifugation and stored at −80°C until further analysis of TOC and TAC capacities. Human peripheral blood MNCs were isolated via density gradient centrifugation using Ficoll Histopaque (Ficoll-Paque™ Plus; GE Healthcare, Opfikon, Switzerland). Differential leukocyte counts were obtained in duplicate by a hematology analyzer (Beckman Coulter AC. T diff; Beckman Coulter, Fullerton, United States; intra-sample coefficient of variation for MNC counts = 3.05%, n = 23).
4
Serum Biomarker Measurement in Pelvic Masses
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Blood samples were collected from patients and healthy women into plastic BD vacutainer rapid serum tubes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) by venipuncture. The blood samples were obtained from the patients on the day preceding the planned surgery. Sera were immediately obtained, aliquoted in sterile polypropylene tubes and stored at -80°C until analysis was carried out. Preoperative serum levels of CA125 and HE4 were measured using the Architect CA125II chemiluminescent microparticle immunoassay (Abbott Diagnostics, Abbott Park, IL, USA) and the HE4 enzyme immunoassay (EIA; Fujirebio DOI:http://dx. doi.org/10.7314/APJCP.2016.17.1.323 Tissue CA125 and HE4 Gene Expression Offers Superior Accuracy in Discriminating Benign from Malignant Pelvic Masses Diagnostics, Malvern, PA, USA), respectively, according to the manufacturer's instructions.
5
Rat C-peptide Measurement by ELISA
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Rat blood was collected from the femoral vein under anesthesia into BD Vacutainer® Rapid Serum Tubes, left for 30 min at room temperature, then centrifuged and serum was collected. The concentration of C‐peptide was measured with rat C‐peptide ELISA kit (ALPCO Diagnostics, Salem, NH, USA).
6
Serum Isolation from Precaval Blood
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Blood was collected via the precaval vein into BD Vacutainer Rapid Serum Tubes (BD, Franklin Lakes, New Jersey, USA) and serum was harvested by centrifugation at 2,000 g for 10 min at 4°C. Samples were stored at −80°C until use.
7
Blood Serum and Tissue Extraction
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When animals were euthanized, blood was collected using BD vacutainer rapid serum tubes (BD Diagnostics, Franklin Lakes, NJ) followed by immediate centrifugation at 3000g for 15 minutes at 4 °C to isolate serum. The serum preparations were then allocated and stored at −80 °C until used. Tissues were harvested, washed in PBS, snap clamp frozen, and kept at −80 °C. Tissue lysates were prepared using tissue protein extraction reagent (Thermo Fisher Scientific, Rockford, IL; catalog number 78510). Protein concentrations were quantified using detergent compatible Bradford assay kit (Thermo Fisher Scientific, Rockford, IL; catalog number 23246).
8
Evaluating Bovine Pneumonia in Feedlot Steers
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Apparently healthy steers were conveniently selected by feedlot staff and presented to the feedlot hospital facility on the day of study enrollment for clinical evaluation. Once it was determined that there had been no prior treatment for clinical BP or other disease during the feeding period, an experienced study veterinarian (ET) examined each steer for inclusion to the study. This included a visual assessment of the steer for clinical signs associated with BP, specifically depression, cough, nasal discharge, and ocular discharge. A complete thoracic auscultation was also performed to detect abnormal lung sounds, as described [56] . Furthermore, a thoracic ultrasonography of the cranioventral portion of both sides of the thorax was performed to detect lung consolidation (> 1 cm deep) or pleural effusion, as described [57] . Rectal temperature was also measured. Finally, whole blood was collected using plain tubes (BD Vacutainer® Rapid Serum Tube, BD Canada, Mississauga, ON, Canada) for haptoglobin analysis, as described [58] .
Steers that did not exhibit any visual signs associated with BP and that had normal lung sounds, no lung consolidation (> 1 cm deep) or pleural effusion detected at thoracic ultrasonography and a rectal temperature < 40 °C were sampled as described below.
Steers that did not exhibit any visual signs associated with BP and that had normal lung sounds, no lung consolidation (> 1 cm deep) or pleural effusion detected at thoracic ultrasonography and a rectal temperature < 40 °C were sampled as described below.
9
Serum Collection for miRNA Analysis
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An 20 mL aliquot of blood was collected from each of the 60 participants (30 patients in the ‘case group’ and 30 individuals in the ‘control group’) with serum collection tubes (BD vacutainer® rapid serum tube, No. 367981; USA). The whole blood was stand for 30 min at room temperature and then was centrifuged at 1800×g for 5 min for serum separation. The resultant serum was aliquoted into microtubes and immediately frozed with liquid nitrogen. All samples were finally stored at −80 °C for following miRNA chip or quantitative reverse transcription–polymerase chain reaction (qRT-PCR) assay.
10
Biomarker Evaluation in Rheumatoid Arthritis
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Morning fasting blood samples were collected in 5 mL BD Vacutainer Rapid Serum Tube, Mississauga, Ontario, Canada (NJ) from each participant, were quickly centrifuged to separate the plasma from the cells, and were then immediately frozen at −80°C until analysis. Before any treatment, SF was obtained from RA patients. Control subjects gave only plasma samples. All samples and indicators examination were detected and analyzed in duplicates. Investigators who were recruited to measure the concentration of the included biomarkers were blinded to the clinical outcome. Levels of TrxR were measured by enzyme-linked immunosorbent assay (ELISA). The coefficients of variation (CVs) of inter-assay and intra-assay were 5.8% to 8.9% and 6.8% to 9.9%, respectively. ACPA levels in plasma and SF in RA patients were tested by ELISA method. Other biomarkers, such as WBC, Hs-CRP, ASO, RF, and ESR, were also tested. In addition, undetectable levels were preset to equal to the lower limit in the experiment.
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